##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545118_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1737728
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89611953078963	31.0	31.0	33.0	30.0	33.0
2	31.212214454736298	31.0	31.0	33.0	30.0	34.0
3	31.341868808006776	31.0	31.0	34.0	28.0	34.0
4	35.190700155605484	37.0	35.0	37.0	33.0	37.0
5	35.07146285264437	37.0	35.0	37.0	32.0	37.0
6	35.16695593326458	37.0	35.0	37.0	32.0	37.0
7	35.569137402401296	36.0	35.0	37.0	35.0	37.0
8	35.63650237551562	37.0	35.0	37.0	35.0	37.0
9	37.4787481124779	39.0	37.0	39.0	35.0	39.0
10	37.01659753425162	39.0	37.0	39.0	33.0	39.0
11	36.555271020550975	39.0	35.0	39.0	32.0	39.0
12	34.89298267623011	35.0	34.0	39.0	30.0	39.0
13	34.35043286406158	35.0	33.0	39.0	27.0	39.0
14	34.93253029242781	35.0	33.0	40.0	27.0	41.0
15	35.48651630174573	35.0	33.0	40.0	30.0	41.0
16	35.75049950279906	35.0	34.0	40.0	31.0	41.0
17	35.60723542464643	35.0	34.0	40.0	31.0	41.0
18	35.70257312997201	36.0	35.0	39.0	31.0	41.0
19	35.54878323880377	36.0	34.0	39.0	31.0	41.0
20	35.22115256242634	35.0	34.0	39.0	30.0	41.0
21	35.03901013277107	35.0	34.0	39.0	30.0	41.0
22	34.868170968068654	35.0	34.0	39.0	29.0	41.0
23	34.85414288081909	35.0	34.0	39.0	29.0	41.0
24	34.962745032594285	35.0	34.0	39.0	30.0	41.0
25	34.88977388866382	35.0	34.0	39.0	30.0	41.0
26	34.63630844412935	35.0	34.0	38.0	29.0	41.0
27	34.60484955067766	35.0	34.0	39.0	29.0	41.0
28	34.71922993702121	35.0	34.0	39.0	29.0	41.0
29	34.73726498048026	35.0	34.0	38.0	29.0	41.0
30	34.69468179139658	35.0	34.0	38.0	29.0	41.0
31	34.397997845462584	35.0	34.0	38.0	29.0	41.0
32	34.198401015578966	35.0	34.0	39.0	27.0	41.0
33	33.942961153874485	35.0	33.0	39.0	26.0	41.0
34	33.74194983334561	35.0	33.0	38.0	24.0	41.0
35	33.482812039628755	35.0	33.0	38.0	23.0	41.0
36	33.197236276333236	35.0	33.0	38.0	23.0	41.0
37	33.18822911295669	35.0	32.0	38.0	23.0	41.0
38	33.27373098666765	35.0	32.0	38.0	23.0	41.0
39	33.290639846972596	35.0	33.0	38.0	23.0	41.0
40	32.824688904132294	35.0	32.0	38.0	22.0	41.0
41	33.01261129474808	35.0	32.0	38.0	23.0	41.0
42	33.02203394317177	35.0	32.0	38.0	23.0	41.0
43	32.99900156986594	35.0	32.0	38.0	23.0	41.0
44	32.95031731087949	35.0	32.0	38.0	23.0	41.0
45	33.03044147300383	35.0	32.0	38.0	23.0	40.0
46	33.059408031636714	35.0	32.0	38.0	23.0	40.0
47	33.00649814010018	35.0	32.0	38.0	23.0	41.0
48	33.05534180262964	35.0	33.0	38.0	23.0	41.0
49	33.1185801230112	35.0	32.0	38.0	23.0	40.0
50	32.87274936008397	35.0	32.0	38.0	23.0	40.0
51	32.5380445040881	35.0	31.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	0.0
12	1.0
13	6.0
14	13.0
15	53.0
16	165.0
17	476.0
18	1242.0
19	2418.0
20	4490.0
21	7016.0
22	10761.0
23	15298.0
24	21476.0
25	28754.0
26	36330.0
27	41567.0
28	45420.0
29	50834.0
30	59837.0
31	73154.0
32	93784.0
33	125022.0
34	239658.0
35	347423.0
36	83159.0
37	99476.0
38	142651.0
39	207114.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.874468271213907	24.753874024012966	31.199301616823803	14.172356087949323
2	31.60172362993518	28.283137522097817	29.257340619475546	10.857798228491456
3	27.69673965085445	26.16312794637596	33.67120746169711	12.46892494107248
4	25.25320418385386	28.971047252504416	32.518783146729525	13.256965416912198
5	21.146635146582206	33.99916442619328	32.073373968768415	12.780826458456099
6	18.896858426635237	41.921462967737185	29.895645348408955	9.286033257218621
7	86.32570805097231	5.1550645440483205	7.471480001473188	1.0477474035061873
8	88.4396177077195	3.255630340306423	7.19456669858574	1.1101852533883323
9	84.85263516499705	4.778538413376547	8.621084542575133	1.747741879051267
10	65.17590785209192	16.219051543164408	12.391409932969948	6.213630671773719
11	62.732257292280494	13.640051837802003	16.70825353565115	6.919437334266353
12	56.17524721935769	20.1093611888627	17.32066238214496	6.394729209634649
13	17.320317103712433	57.92845600692398	17.253448180612846	7.497778708750737
14	8.25497431128462	57.101111336181496	25.65211586623453	8.991798486299352
15	7.176727312905126	21.698332535356514	63.141009409988214	7.9839307417501475
16	7.562000497200942	14.041553108426635	61.86779519004125	16.528651204331172
17	8.026054710518563	15.007181791396581	31.567023147466116	45.39974035061874
18	14.666909896140249	21.769920263700648	46.73533487404243	16.827834966116676
19	33.788774767972896	17.138067637743077	30.5950068134944	18.47815078078963
20	34.02367919490277	19.112427261343548	29.478951826753093	17.38494171700059
21	15.067260238656452	34.34035706393636	32.91752218989393	17.67486050751326
22	17.60528690335887	24.00364153653506	21.038562997200945	37.35250856290513
23	11.521308282999412	34.254670466263995	25.357305631261053	28.866715619475546
24	8.953184848261639	19.287310787418974	59.883077213464944	11.876427150854449
25	7.703507108131998	23.953691256629345	51.579361096788446	16.763440538450205
26	8.43043330141426	43.54127918753683	30.72414094725987	17.30414656378904
27	8.133666488656452	54.26677822996464	26.65589781599882	10.943657465380083
28	7.228921902622274	36.73699221051856	44.69686855480259	11.337217332056571
29	8.052756242634059	25.766460573806715	42.37251169342958	23.80827149012964
30	12.644556570418386	44.64254474808486	29.84460168680024	12.868296994696523
31	33.87676322186211	29.673113398644666	27.199768893635827	9.250354485857395
32	37.850687794637594	20.91823346346494	31.188310253388334	10.042768488509134
33	38.35824709045374	27.02845324469652	20.165296294932233	14.448003369917501
34	19.99662778064231	25.665811910724806	24.115224016647023	30.222336291985858
35	19.372537013847968	24.526393083382438	35.61345619107248	20.48761371169711
36	41.072020477312904	24.897394759133764	22.78636242265763	11.244222340895698
37	15.01144022539776	47.84649841632292	26.978560511196232	10.163500847083087
38	16.290293993076016	44.431982450648206	22.14759732248085	17.130126233794932
39	20.559143893635827	40.90686229375368	25.854161295668828	12.67983251694166
40	28.78425162050678	22.492012558927517	25.6784721199175	23.045263700648203
41	12.26710969725987	23.7382950611373	27.490608426635237	36.50398681496759
42	16.495274289186803	19.2676874631703	26.58977699616971	37.64726125147318
43	17.402205638626988	19.4638056128462	29.900133968031824	33.233854780494994
44	10.540890173836182	32.23318033662345	35.76762301119623	21.458306478344134
45	9.92163330509723	56.62813743002357	20.581817177371832	12.868412087507366
46	17.687232984678843	43.717946652180316	23.50264253093695	15.092177832203891
47	18.621038505450795	29.133903579846788	24.91908975397761	27.325968160724806
48	19.285699488067177	25.70609439451974	40.61423882218621	14.39396729522687
49	23.90834468915734	26.033015523718326	38.59902125073659	11.459618536387744
50	18.85410144740719	46.7306736152033	22.861748213759576	11.553476723629934
51	17.375734292133178	40.39510211034178	23.5273299388627	18.701833658662345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2788.0
1	3735.0
2	4682.0
3	9624.5
4	14567.0
5	11834.5
6	9102.0
7	9635.0
8	10168.0
9	10821.0
10	11474.0
11	11521.5
12	11569.0
13	11320.5
14	11072.0
15	10507.5
16	9943.0
17	9211.0
18	8479.0
19	8592.5
20	8706.0
21	8562.5
22	8419.0
23	9199.0
24	9979.0
25	11603.0
26	16968.5
27	20710.0
28	24135.5
29	27561.0
30	33006.5
31	38452.0
32	44979.0
33	51506.0
34	57472.0
35	63438.0
36	59537.0
37	55636.0
38	76620.5
39	97605.0
40	147579.5
41	197554.0
42	241603.5
43	285653.0
44	279551.5
45	273450.0
46	225926.5
47	178403.0
48	150323.0
49	122243.0
50	98806.0
51	75369.0
52	59704.5
53	44040.0
54	32239.0
55	20438.0
56	16502.5
57	12567.0
58	11205.0
59	9843.0
60	9069.0
61	8295.0
62	7393.5
63	6492.0
64	5553.0
65	4614.0
66	3797.5
67	2981.0
68	2619.5
69	2258.0
70	1985.5
71	1713.0
72	1411.5
73	1110.0
74	968.0
75	617.5
76	409.0
77	306.0
78	203.0
79	152.5
80	102.0
81	74.5
82	47.0
83	33.0
84	19.0
85	15.0
86	11.0
87	7.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1737728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.759000684807189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81924553847968	12.893895464713086
2	7.370416753073185	2.323008053179894
3	2.4638049339132664	1.1648131092231149
4	1.3147317071935887	0.8287543149600596
5	0.8330036568219571	0.6563652599152058
6	0.6148983494912966	0.5814110106432092
7	0.46681876519035864	0.5149618068220996
8	0.3764806675708139	0.47463672784520994
9	0.28882151905461684	0.4096384664911884
>10	2.8382325324373245	9.469747912061175
>50	0.4170372179139104	4.649493651397102
>100	0.9546414318010467	40.44763203744769
>500	0.22391014005007276	21.501244313559805
>1k	0.017590321967927154	3.659117826388768
>5k	3.664650409984824E-4	0.42528004535238134
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7364	0.4237717295226871	No Hit
TTTGACGGGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG	2791	0.16061201753093693	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2480	0.14271508544490277	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2175	0.12516343179139658	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1893	0.10893534546258102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014271508544490276	0.0
2	0.0	0.0	0.0	0.07371694534472599	0.0
3	0.0	0.0	0.0	0.11043155200353565	0.0
4	0.0	0.0	0.0	0.1739052371832646	0.0
5	0.0	0.0	0.0	0.3020610820565704	0.0
6	0.0	0.0	0.0	0.4239443687389511	0.0
7	0.0	0.0	0.0	0.5047395219505009	0.0
8	0.0	0.0	0.0	0.6922257108131997	0.0
9	0.0	0.0	0.0	0.768935069239835	0.0
10	0.0	0.0	0.0	0.9062983389805539	0.0
11	0.0	0.0	0.0	1.0544803329404833	0.0
12	0.0	0.0	0.0	1.1794135791101945	0.0
13	0.0	0.0	0.0	1.229306312610489	0.0
14	0.0	0.0	0.0	1.2488720904537418	0.0
15	0.0	0.0	0.0	1.281673541543901	0.0
16	0.0	0.0	0.0	1.3636196228638775	0.0
17	0.0	0.0	0.0	1.453737293753683	0.0
18	0.0	0.0	0.0	1.6062352681202121	0.0
19	0.0	0.0	0.0	1.657911940188568	0.0
20	0.0	0.0	0.0	1.7171271913671184	0.0
21	0.0	0.0	0.0	1.7994760975250441	0.0
22	0.0	0.0	0.0	1.8746317030053035	0.0
23	0.0	0.0	0.0	1.9710794784914556	0.0
24	0.0	0.0	0.0	2.0392144225103124	0.0
25	5.754640542133176E-5	0.0	0.0	2.094171239687684	0.0
26	5.754640542133176E-5	0.0	0.0	2.1555157078668237	0.0
27	5.754640542133176E-5	0.0	0.0	2.210184793017089	0.0
28	5.754640542133176E-5	0.0	0.0	2.2681915696817914	0.0
29	5.754640542133176E-5	0.0	0.0	2.3335642862404242	0.0
30	5.754640542133176E-5	0.0	0.0	2.4338101244843844	0.0
31	5.754640542133176E-5	0.0	0.0	2.5300277143488508	0.0
32	5.754640542133176E-5	0.0	0.0	2.6112832388037712	0.0
33	5.754640542133176E-5	0.0	0.0	2.688337875662935	0.0
34	5.754640542133176E-5	0.0	0.0	2.774312205362404	0.0
35	5.754640542133176E-5	0.0	0.0	2.900511472451385	0.0
36	5.754640542133176E-5	0.0	0.0	2.9996639289923395	0.0
37	5.754640542133176E-5	0.0	0.0	3.0969749005598115	0.0
38	5.754640542133176E-5	0.0	0.0	3.18289168385386	0.0
39	5.754640542133176E-5	0.0	0.0	3.2669669821744254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTC	30	2.1661454E-6	45.000004	16
CGGGTAC	30	2.1661454E-6	45.000004	6
AGCGTCG	35	1.212411E-7	45.000004	17
TAATACG	20	7.034284E-4	45.000004	1
CGAACGG	20	7.034284E-4	45.000004	2
GCGACTA	20	7.034284E-4	45.000004	21
GTCGATG	60	0.0	45.000004	1
GTCGATA	40	6.8193913E-9	45.000004	9
GGTCGCG	20	7.034284E-4	45.000004	28
CACGGAT	35	1.212411E-7	45.000004	19
CGAAACA	20	7.034284E-4	45.000004	17
ACGTTAG	40	6.8193913E-9	45.000004	1
ACGTTAC	20	7.034284E-4	45.000004	1
TCTTCGA	65	0.0	45.000004	27
TAGCGAA	65	0.0	45.000004	1
GACCGAC	85	0.0	45.000004	31
ACCGACA	85	0.0	45.000004	32
CCGTGAG	40	6.8193913E-9	45.000004	2
GCACGGA	20	7.034284E-4	45.000004	3
CCTACCG	20	7.034284E-4	45.000004	37
>>END_MODULE