FastQCFastQC Report
Thu 26 May 2016
SRR1545116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545116_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1339227
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47230.35266612754969845No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT19920.14874252087211504No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15590.11641043676688119No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14570.10879410286680302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14350.10715136418247243No Hit
TTGATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA14050.10491126597656708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG351.2121382E-745.0000041
ATTCGGA351.2121382E-745.00000414
TTGCGAG351.2121382E-745.0000041
GCGATAC351.2121382E-745.0000049
CTCGCAC351.2121382E-745.00000413
CTCGAGG351.2121382E-745.0000042
CGCACAA351.2121382E-745.00000415
GCTACGG1450.045.0000042
AATACGG1400.045.0000042
AAAGCGT700.045.0000045
CGGACAA207.033705E-445.017
CCTTCGT406.8157533E-945.023
CTTCGTC406.8157533E-945.024
GTCATCG253.891152E-545.09
CGGGTGC551.8189894E-1245.06
CGGGTCG207.033705E-445.06
CGGGTCA551.8189894E-1245.06
CGGGTAT1100.045.06
AGCGTCA207.033705E-445.035
CTATGCG750.045.01