##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545116_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1339227
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85842504668738	31.0	31.0	33.0	30.0	33.0
2	31.1868981136133	31.0	31.0	33.0	30.0	34.0
3	31.349242510791672	31.0	31.0	34.0	30.0	34.0
4	35.19524546622791	37.0	35.0	37.0	33.0	37.0
5	35.083564623473094	37.0	35.0	37.0	32.0	37.0
6	35.15665454773537	37.0	35.0	37.0	32.0	37.0
7	35.52739229421151	36.0	35.0	37.0	35.0	37.0
8	35.5983615921722	37.0	35.0	37.0	35.0	37.0
9	37.46867409333892	39.0	37.0	39.0	35.0	39.0
10	36.91560504679192	39.0	37.0	39.0	32.0	39.0
11	36.54565880168187	39.0	35.0	39.0	32.0	39.0
12	35.17976340082749	35.0	35.0	39.0	31.0	39.0
13	34.72279979420964	35.0	33.0	39.0	30.0	39.0
14	35.38242732561395	36.0	33.0	40.0	27.0	41.0
15	35.824701114896875	36.0	34.0	40.0	30.0	41.0
16	36.033061609420955	36.0	34.0	40.0	32.0	41.0
17	35.900917469555196	35.0	34.0	40.0	31.0	41.0
18	36.012710317220304	36.0	35.0	40.0	31.0	41.0
19	35.87952826518581	36.0	35.0	40.0	31.0	41.0
20	35.586918423837034	35.0	34.0	40.0	30.0	41.0
21	35.445222505221295	35.0	34.0	40.0	30.0	41.0
22	35.27957246979041	35.0	34.0	40.0	30.0	41.0
23	35.2383733302868	35.0	34.0	40.0	30.0	41.0
24	35.33571530442561	35.0	34.0	40.0	30.0	41.0
25	35.26453917073058	35.0	34.0	40.0	30.0	41.0
26	35.014368736592075	35.0	34.0	39.0	29.0	41.0
27	35.011868040294885	35.0	34.0	39.0	29.0	41.0
28	35.13268923042919	36.0	34.0	39.0	29.0	41.0
29	35.101819930452415	36.0	34.0	39.0	29.0	41.0
30	35.08464360410894	36.0	34.0	39.0	29.0	41.0
31	34.84297807615886	35.0	34.0	39.0	29.0	41.0
32	34.68463897457264	35.0	34.0	40.0	29.0	41.0
33	34.456151197668504	35.0	33.0	40.0	27.0	41.0
34	34.261416473831545	35.0	34.0	40.0	26.0	41.0
35	34.03357832540712	35.0	33.0	40.0	24.0	41.0
36	33.73164892882237	35.0	33.0	39.0	23.0	41.0
37	33.78817332685198	35.0	33.0	39.0	23.0	41.0
38	33.86459726394405	35.0	33.0	39.0	24.0	41.0
39	33.90055457364584	35.0	33.0	39.0	24.0	41.0
40	33.53017972307906	35.0	33.0	39.0	23.0	41.0
41	33.643383832613885	35.0	33.0	39.0	23.0	41.0
42	33.63742218458857	35.0	33.0	39.0	23.0	41.0
43	33.61048127016555	35.0	33.0	39.0	23.0	41.0
44	33.59387392876637	35.0	33.0	39.0	23.0	41.0
45	33.67211831900044	35.0	33.0	39.0	23.0	41.0
46	33.67764538797381	35.0	33.0	39.0	23.0	41.0
47	33.601873319459656	35.0	33.0	39.0	23.0	41.0
48	33.69660557918859	35.0	33.0	39.0	23.0	41.0
49	33.73272940285702	35.0	33.0	39.0	24.0	41.0
50	33.52840631199938	35.0	33.0	39.0	24.0	41.0
51	33.23229445045538	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	13.0
15	34.0
16	94.0
17	333.0
18	782.0
19	1549.0
20	2864.0
21	4705.0
22	7030.0
23	10230.0
24	14641.0
25	19835.0
26	25111.0
27	29154.0
28	31839.0
29	36066.0
30	43549.0
31	53579.0
32	68622.0
33	90583.0
34	164486.0
35	245131.0
36	74376.0
37	90305.0
38	130050.0
39	194197.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.607507166447512	24.012807388142562	32.42236006293183	12.957325382478102
2	29.993122898507867	25.938321135998603	32.29019426878341	11.778361696710117
3	26.70137325487016	25.851704005370262	34.18457065157737	13.262352088182213
4	23.716143715740497	30.23759228271234	32.95154592910687	13.094718072440296
5	22.359838922005007	32.81982815459963	32.583049774235434	12.237283149159927
6	19.071972115257534	42.39460524616066	28.823418285324294	9.710004353257514
7	88.34924923108629	4.199064086969573	6.578645741162625	0.8730409407815106
8	91.06409891676319	2.0918783746146095	5.817684380616579	1.026338328005633
9	87.47001068526843	3.6887697156643346	7.166895530033369	1.6743240690338532
10	58.20872787062985	22.239321638527297	12.75026563831225	6.8016848525306015
11	54.3406009586127	15.20660799102766	20.15125143086273	10.30153961949692
12	51.26778357963213	19.840400469823262	21.0749185910977	7.816897359446904
13	17.27123183747042	55.781133444890216	17.29228876060593	9.655345957033424
14	9.242495857685068	53.38557242349504	24.247271000360655	13.12466071845923
15	7.374925983421781	22.86856522456611	58.883520120188734	10.872988671823373
16	7.860653944402256	15.611169727014165	56.149554929821456	20.378621398762125
17	8.012980622403818	17.679676410347163	30.88968487045139	43.41765809679763
18	14.670179140653527	21.714317289003283	45.777825566539505	17.837678003803685
19	31.51444826007839	21.899797420452245	27.194045520288945	19.391708799180424
20	33.515154637712655	18.816600919784324	30.2433418681075	17.424902574395528
21	14.48454966932417	35.083074041966	31.276773840431833	19.155602448278
22	18.904114089695025	23.417762634713906	20.480097847489635	37.19802542810143
23	11.476471128494273	35.575074277923015	24.028189395823112	28.920265197759605
24	8.775808731454788	19.780365837905002	59.15546804238564	12.288357388254568
25	8.747508824120183	27.23742875554331	47.423999068119144	16.59106335221736
26	10.210143612695981	41.948377683544315	28.54758752623715	19.293891177522557
27	7.676816551637623	51.43146008854361	25.491048194219502	15.400675165599258
28	7.485213485092519	33.48536133157411	43.881806445061216	15.147618738272154
29	7.361261384365757	23.018054444840192	42.90758773531299	26.713096435481066
30	12.173440350291624	44.55577732527794	30.504462648975867	12.766319675454572
31	31.03902475084508	32.57222263290689	26.248276057755703	10.140476558492324
32	34.440315196751556	21.975811419572633	28.722240516357573	14.861632867318237
33	35.10353360557993	28.77570419353851	19.830096018076098	16.290666182805456
34	17.17401157533413	24.264071736904945	25.58274288078123	32.9791738069797
35	17.608067937698387	24.743602092849084	36.5810277122549	21.067302257197625
36	37.3337007094391	27.665511522691823	21.5813301255127	13.419457642356376
37	14.157495331261991	46.09367941357216	28.611355655165255	11.137469600000598
38	14.296530759908515	42.26938375645055	26.574434356535527	16.859651127105412
39	17.153477341779997	42.9024355094394	26.346093679413574	13.597993469367031
40	27.52737213332766	24.374433908515883	27.01692842214203	21.08126553601443
41	11.871400442195386	24.374657918336474	28.617329250381	35.136612389087134
42	14.352533215056148	21.217314167053082	25.989619384913837	38.44053323297693
43	14.949370047049529	21.54996875063003	30.65059172194109	32.850069480379354
44	10.712970989981534	30.293520067919776	36.46528930494979	22.52821963714889
45	9.850757190528567	54.68318664423582	20.495405185229988	14.970650980005631
46	16.179855991553335	45.1792713259216	23.96852811360583	14.672344568919234
47	15.20302383389821	31.536849242137443	25.991336793538363	27.268790130425984
48	15.946437758498	25.992008823000134	41.3760325919355	16.685520826566368
49	20.629437727883325	26.774400456382676	39.84044527178738	12.755716543946619
50	16.91296546440596	45.443826924039016	24.85971384985518	12.783493761699846
51	13.87987249361012	38.984279737490354	26.686737946591577	20.449109822307943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1690.0
1	1959.5
2	2229.0
3	5652.0
4	9075.0
5	7364.0
6	5653.0
7	6183.5
8	6714.0
9	7055.0
10	7396.0
11	7399.5
12	7403.0
13	7020.0
14	6637.0
15	6425.0
16	6213.0
17	5978.0
18	5743.0
19	5019.5
20	4296.0
21	4750.0
22	5204.0
23	5491.5
24	5779.0
25	6339.0
26	11357.0
27	15815.0
28	18697.0
29	21579.0
30	26661.5
31	31744.0
32	39665.5
33	47587.0
34	56341.0
35	65095.0
36	71354.0
37	77613.0
38	86416.5
39	95220.0
40	123200.5
41	151181.0
42	181003.5
43	210826.0
44	201173.5
45	191521.0
46	162349.5
47	133178.0
48	109067.0
49	84956.0
50	69662.0
51	54368.0
52	43040.0
53	31712.0
54	22914.5
55	14117.0
56	11096.5
57	8076.0
58	7189.0
59	6302.0
60	5618.0
61	4934.0
62	4371.0
63	3808.0
64	3320.5
65	2833.0
66	2418.5
67	2004.0
68	1645.5
69	1287.0
70	1110.0
71	933.0
72	829.0
73	725.0
74	561.0
75	320.5
76	244.0
77	186.0
78	128.0
79	95.0
80	62.0
81	44.5
82	27.0
83	22.0
84	17.0
85	11.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1339227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.864348551395832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36432423000298	12.907919992335485
2	7.280212652015912	2.309916620797245
3	2.475419175181122	1.178127378176463
4	1.3041977475768232	0.8276099059001634
5	0.8161827654278394	0.6474103936194693
6	0.605861050544702	0.57669545277336
7	0.46037390286418256	0.5112472441302689
8	0.38224969537441766	0.48513139208677125
9	0.322211878428986	0.46005133921176733
>10	2.9380507456197584	9.756192559707584
>50	0.550971260399921	6.24918523516555
>100	1.4376242398600707	56.68367680873132
>500	0.05618301627039358	5.8723646234443745
>1k	0.006137640432900138	1.5344710539201931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4723	0.35266612754969845	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT	1992	0.14874252087211504	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1559	0.11641043676688119	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1457	0.10879410286680302	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1435	0.10715136418247243	No Hit
TTGATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1405	0.10491126597656708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026059809128698867	0.0
2	0.0	0.0	0.0	0.10005771986377215	0.0
3	0.0	0.0	0.0	0.14090217715144632	0.0
4	0.0	0.0	0.0	0.20519299566092977	0.0
5	0.0	0.0	0.0	0.341316296639778	0.0
6	0.0	0.0	0.0	0.477066247917642	0.0
7	0.0	0.0	0.0	0.5577097833302346	0.0
8	0.0	0.0	0.0	0.7772394075089585	0.0
9	0.0	0.0	0.0	0.8529547268685592	0.0
10	0.0	0.0	0.0	0.9884806683258327	0.0
11	0.0	0.0	0.0	1.123110570500744	0.0
12	0.0	0.0	0.0	1.2375049188823104	0.0
13	0.0	0.0	0.0	1.2830535824023859	0.0
14	0.0	0.0	0.0	1.2988089397839202	0.0
15	0.0	0.0	0.0	1.3286022459224613	0.0
16	0.0	0.0	0.0	1.4017787873153693	0.0
17	0.0	0.0	0.0	1.4942201732790632	0.0
18	0.0	0.0	0.0	1.6486376096061384	0.0
19	0.0	0.0	0.0	1.7062828034381028	0.0
20	0.0	0.0	0.0	1.7568343529513668	0.0
21	0.0	0.0	0.0	1.8402406761512424	0.0
22	0.0	0.0	0.0	1.9258870975570235	0.0
23	0.0	0.0	0.0	2.037817337912094	0.0
24	0.0	0.0	0.0	2.119655592367836	0.0
25	0.0	0.0	0.0	2.185141129920469	0.0
26	0.0	0.0	0.0	2.252717425798614	0.0
27	0.0	0.0	0.0	2.323355189224829	0.0
28	0.0	0.0	0.0	2.3904834654617924	0.0
29	0.0	0.0	0.0	2.4747858279440305	0.0
30	0.0	0.0	0.0	2.592913673335439	0.0
31	0.0	0.0	0.0	2.69513682146492	0.0
32	0.0	0.0	0.0	2.782052631854047	0.0
33	0.0	0.0	0.0	2.8655336249941197	0.0
34	0.0	0.0	0.0	2.961111148446081	0.0
35	0.0	0.0	0.0	3.1052241330259918	0.0
36	0.0	0.0	0.0	3.201548355879922	0.0
37	0.0	0.0	0.0	3.3129559066536145	0.0
38	0.0	0.0	0.0	3.404053233693765	0.0
39	0.0	0.0	0.0	3.5035882639761593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2121382E-7	45.000004	1
ATTCGGA	35	1.2121382E-7	45.000004	14
TTGCGAG	35	1.2121382E-7	45.000004	1
GCGATAC	35	1.2121382E-7	45.000004	9
CTCGCAC	35	1.2121382E-7	45.000004	13
CTCGAGG	35	1.2121382E-7	45.000004	2
CGCACAA	35	1.2121382E-7	45.000004	15
GCTACGG	145	0.0	45.000004	2
AATACGG	140	0.0	45.000004	2
AAAGCGT	70	0.0	45.000004	5
CGGACAA	20	7.033705E-4	45.0	17
CCTTCGT	40	6.8157533E-9	45.0	23
CTTCGTC	40	6.8157533E-9	45.0	24
GTCATCG	25	3.891152E-5	45.0	9
CGGGTGC	55	1.8189894E-12	45.0	6
CGGGTCG	20	7.033705E-4	45.0	6
CGGGTCA	55	1.8189894E-12	45.0	6
CGGGTAT	110	0.0	45.0	6
AGCGTCA	20	7.033705E-4	45.0	35
CTATGCG	75	0.0	45.0	1
>>END_MODULE