FastQCFastQC Report
Thu 26 May 2016
SRR1545114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545114_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1809251
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107560.5945001550365316No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24850.13734965463608972No Hit
GTAATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA18420.10181008605218403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.034361E-445.0000041
GTCAAGT302.1661963E-645.0000049
CCGAATC207.034361E-445.00000414
CGATCGT302.1661963E-645.00000410
ATCGCGA207.034361E-445.00000411
GTCGGAT302.1661963E-645.0000049
TCACGAC1350.045.025
CGCATCG253.8916973E-545.021
CCGCTCG253.8916973E-545.019
TCGATAT551.8189894E-1245.045
CGTAGAT253.8916973E-545.037
CAGGCGA253.8916973E-545.05
CGAATTA253.8916973E-545.040
TGTTACG850.045.01
CCGAATA253.8916973E-545.019
CCGTTCA551.8189894E-1245.012
TACGCCC650.044.99999618
ACATACG17250.043.56521617
CATACGA17550.043.4615418
ACGGGTA1400.043.3928575
Produced by FastQC (version 0.11.5)