FastQCFastQC Report
Thu 26 May 2016
SRR1545113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545113_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1115245
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53790.48231554501477253No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25130.22533165358284502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19140.17162148227519514No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16820.15081887836305027No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14010.12562262103842653No Hit
GTTATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA13270.11898730772162171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA302.1654432E-645.00000432
GCGACAG302.1654432E-645.0000041
CACGGGC302.1654432E-645.0000044
CGACGCA302.1654432E-645.00000443
TCCGATG453.8562575E-1045.0000041
CGTGCAT453.8562575E-1045.00000423
ACACCAT302.1654432E-645.00000439
CAACCGT302.1654432E-645.0000049
AATGCGT600.045.00000422
CTAACGG453.8562575E-1045.0000041
ACGGCAT302.1654432E-645.00000418
ACGTGCA453.8562575E-1045.00000422
ACGCTAG302.1654432E-645.0000041
CTCGATG650.045.0000041
ATGTTCG302.1654432E-645.0000041
TGATGCG650.045.0000041
ATACCGG650.045.0000042
CTGTGCG302.1654432E-645.0000041
TACGTAG650.045.0000041
TGTCACG406.8139343E-945.01