##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545113_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1115245 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.907956547664416 31.0 31.0 33.0 30.0 33.0 2 31.21485682518191 31.0 31.0 33.0 30.0 34.0 3 31.339886751341634 31.0 31.0 34.0 28.0 34.0 4 35.22567148922434 37.0 35.0 37.0 33.0 37.0 5 35.11978802863945 37.0 35.0 37.0 32.0 37.0 6 35.20859631740111 37.0 35.0 37.0 32.0 37.0 7 35.55134970342839 36.0 35.0 37.0 35.0 37.0 8 35.59197306421459 37.0 35.0 37.0 35.0 37.0 9 37.43778452268336 39.0 37.0 39.0 35.0 39.0 10 36.99091500073975 39.0 37.0 39.0 33.0 39.0 11 36.583647539329924 39.0 35.0 39.0 32.0 39.0 12 35.109968661594536 35.0 35.0 39.0 31.0 39.0 13 34.651325941833406 35.0 33.0 39.0 30.0 39.0 14 35.25757479298271 35.0 33.0 40.0 27.0 41.0 15 35.722049415150934 35.0 34.0 40.0 30.0 41.0 16 35.941306170393055 35.0 34.0 40.0 32.0 41.0 17 35.777320678415954 35.0 34.0 40.0 31.0 41.0 18 35.86551385569987 36.0 35.0 39.0 31.0 41.0 19 35.70544768189949 36.0 35.0 39.0 31.0 41.0 20 35.394202170823455 35.0 34.0 39.0 30.0 41.0 21 35.241766607337404 35.0 34.0 39.0 30.0 41.0 22 35.083501831436145 35.0 34.0 39.0 30.0 41.0 23 35.06559455545642 35.0 34.0 39.0 30.0 41.0 24 35.141913211895144 35.0 34.0 39.0 30.0 41.0 25 35.051395881622426 35.0 34.0 39.0 30.0 41.0 26 34.83019067559146 35.0 34.0 39.0 29.0 41.0 27 34.80751314733534 35.0 34.0 39.0 29.0 41.0 28 34.8811480885366 35.0 34.0 39.0 29.0 41.0 29 34.85657052934557 35.0 34.0 39.0 29.0 41.0 30 34.80472990239813 35.0 34.0 39.0 29.0 41.0 31 34.48760586238898 35.0 34.0 39.0 29.0 41.0 32 34.30484422705325 35.0 34.0 39.0 27.0 41.0 33 34.02141950871781 35.0 33.0 39.0 25.0 41.0 34 33.79341938318486 35.0 33.0 39.0 24.0 41.0 35 33.59097866388103 35.0 33.0 39.0 23.0 41.0 36 33.292810996686825 35.0 33.0 39.0 22.0 41.0 37 33.305340082224085 35.0 33.0 39.0 23.0 41.0 38 33.377845226833564 35.0 33.0 39.0 23.0 41.0 39 33.365710673439466 35.0 33.0 39.0 23.0 41.0 40 33.01372523526221 35.0 32.0 39.0 22.0 41.0 41 33.14884442431932 35.0 32.0 39.0 23.0 41.0 42 33.12054750301503 35.0 32.0 39.0 23.0 41.0 43 33.07192051970643 35.0 32.0 39.0 23.0 41.0 44 33.05487493779394 35.0 32.0 38.0 23.0 41.0 45 33.16185950172384 35.0 33.0 38.0 23.0 41.0 46 33.192811445018805 35.0 33.0 38.0 23.0 41.0 47 33.09716609354895 35.0 32.0 38.0 23.0 41.0 48 33.12973472196692 35.0 33.0 38.0 23.0 41.0 49 33.19664289012728 35.0 33.0 38.0 23.0 41.0 50 32.972277840295185 35.0 32.0 38.0 23.0 40.0 51 32.62604629476034 35.0 32.0 38.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 1.0 11 1.0 12 1.0 13 2.0 14 9.0 15 33.0 16 111.0 17 284.0 18 680.0 19 1397.0 20 2587.0 21 4403.0 22 6458.0 23 9428.0 24 13447.0 25 18759.0 26 23903.0 27 26611.0 28 28754.0 29 31680.0 30 37049.0 31 45441.0 32 57545.0 33 77934.0 34 147358.0 35 213802.0 36 57910.0 37 69251.0 38 99658.0 39 140698.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.297835901528366 24.703899143237585 28.783720169110822 14.214544786123229 2 32.04721832422472 25.355235845038532 30.443265829481415 12.15428000125533 3 27.55269021605118 26.082788983586568 34.64844047720456 11.716080323157692 4 25.877811601935 27.962824312146655 33.60024030594174 12.559123779976597 5 22.78189994126851 33.638796856296146 31.131814085694177 12.447489116741165 6 19.712708866661586 41.51894875117127 29.275809351308457 9.492533030858691 7 84.54662428435007 5.616972055467633 8.493649377491044 1.342754282691247 8 87.05853870674157 3.88596227734713 7.937000390048824 1.1184986258624785 9 83.16360978977714 5.634098337136683 9.384126357885487 1.8181655152006957 10 62.38369147586405 17.489071907966412 13.539536155732595 6.5877004604369445 11 59.26527354975812 15.553443413779036 18.59618290151491 6.5851001349479255 12 53.20875682025026 21.06335379221606 18.38914319275137 7.3387461947823125 13 17.354123981725987 55.91255733045205 18.131441970150057 8.601876717671901 14 9.401790637931576 54.78778205685746 25.910181170953468 9.900246134257495 15 8.253433102143475 22.337333949042588 60.77086200789961 8.638370940914328 16 8.865585588816808 16.016301350824257 58.894861667167305 16.22325139319163 17 9.473792754058525 16.512246188057333 31.328721491690164 42.68523956619398 18 15.16931257257374 22.446368286789 45.7108527722608 16.673466368376456 19 33.09667382503396 18.94247452353519 28.631376962012833 19.32947468941802 20 32.86919017794296 19.75682473357872 30.781846141430808 16.59213894704751 21 15.459204031401171 34.70959295939458 32.90783639469354 16.923366614510712 22 18.58667826352057 25.015758869127414 22.326663647897995 34.07089921945402 23 12.778985783392887 34.16217961075817 25.66978556281355 27.389049043035385 24 9.97422091110025 20.691327914494124 57.38308622768989 11.951364946715744 25 9.124093808983675 24.427995642213148 51.51316526861811 14.934745280185071 26 9.07961927648185 41.862281382117835 30.34875744791503 18.709341893485288 27 9.285313989302798 52.55455079377177 26.588148792417808 11.57198642450762 28 7.891987859169958 36.81971226053468 43.07753004945102 12.210769830844344 29 8.69835775995409 26.005496550085404 42.77302296804738 22.523122721913122 30 13.03677667239037 44.253594501656586 29.548305529278316 13.16132329667472 31 32.39225461669857 31.27922564100265 26.048446753852296 10.280072988446484 32 36.140847975108606 22.118099610399508 30.958040609910825 10.783011804581056 33 36.31865643871974 28.502526350712177 20.159516518791833 15.019300691776246 34 18.973588763007232 27.777932203237853 24.26193347650068 28.986545557254235 35 18.447516016660018 25.1854076906868 34.98208913736443 21.384987155288748 36 38.91396060955216 26.251092809203357 23.04776080592157 11.78718577532291 37 15.640419818066883 45.793525189532346 28.499388026846116 10.066666965554653 38 16.038448950678998 43.81135983573116 22.563651932983337 17.5865392806065 39 19.437971028787395 40.191975754206474 27.267640742617093 13.102412474389036 40 27.96847329510556 24.35868351797139 24.503584414187017 23.169258772736036 41 13.069594573389704 23.97509067514313 28.44298786365328 34.512326887813884 42 16.221906397249036 21.30186640603634 26.709108760855237 35.76711843585939 43 16.899067021147822 21.29827975018942 30.36857372146927 31.434079507193484 44 10.641607897816174 31.954144604997108 35.700137637918125 21.70410985926859 45 10.548982510569427 54.79011338315796 21.5231630717914 13.137741034481213 46 19.48414922281651 42.85695071486534 23.34590157319692 14.312998489121226 47 17.63271747463562 30.554272827943635 25.05521208344355 26.757797613977196 48 17.543858076028137 28.904321471963563 39.5510403543616 14.000780097646706 49 22.13549489125708 28.17640966783084 37.692614627279205 11.995480813632879 50 19.714412528188873 44.69134584777336 23.452156252661972 12.142085371375796 51 15.880277427829759 40.228559643845074 24.026559186546454 19.864603741778712 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2316.0 1 2622.5 2 2929.0 3 7114.5 4 11300.0 5 9071.0 6 6842.0 7 7206.0 8 7570.0 9 7998.5 10 8427.0 11 8198.0 12 7969.0 13 7632.0 14 7295.0 15 6635.5 16 5976.0 17 5702.5 18 5429.0 19 5232.5 20 5036.0 21 5325.5 22 5615.0 23 6203.0 24 6791.0 25 7616.5 26 11464.5 27 14487.0 28 17095.0 29 19703.0 30 24235.5 31 28768.0 32 31471.5 33 34175.0 34 37761.5 35 41348.0 36 42883.0 37 44418.0 38 54854.0 39 65290.0 40 93369.0 41 121448.0 42 149157.0 43 176866.0 44 170147.0 45 163428.0 46 139976.5 47 116525.0 48 94735.5 49 72946.0 50 59831.5 51 46717.0 52 34912.5 53 23108.0 54 18546.5 55 13985.0 56 11538.5 57 9092.0 58 8452.0 59 7812.0 60 7301.0 61 6790.0 62 5727.0 63 4664.0 64 3958.0 65 3252.0 66 2786.5 67 2321.0 68 2174.5 69 2028.0 70 1824.0 71 1620.0 72 1336.0 73 1052.0 74 889.0 75 561.0 76 396.0 77 272.5 78 149.0 79 123.0 80 97.0 81 70.5 82 44.0 83 34.5 84 25.0 85 19.5 86 14.0 87 11.0 88 8.0 89 4.5 90 1.0 91 2.5 92 4.0 93 2.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1115245.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.79331042587048 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99450798875895 14.233671130087641 2 7.389834731789953 2.6297924675723583 3 2.890807834601593 1.5431112354781373 4 1.598539376818751 1.1377322943885433 5 1.0460541113151662 0.9306382762444412 6 0.7820977239249731 0.8349664551098278 7 0.5682005467235746 0.7077118098401319 8 0.44428500092475115 0.63242407512098 9 0.393595344809482 0.6303027737135377 >10 3.0107933246794807 10.7739872114143 >50 0.5278313658538405 6.805415448210113 >100 1.30744436024709 52.718332624537 >500 0.040952433557623465 4.769161450533355 >1k 0.004550270395291496 1.16885567447806 >5k 5.055855994768329E-4 0.4838970732715681 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5379 0.48231554501477253 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2513 0.22533165358284502 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1914 0.17162148227519514 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1682 0.15081887836305027 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1401 0.12562262103842653 No Hit GTTATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 1327 0.11898730772162171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.020802603912144866 0.0 2 0.0 0.0 0.0 0.07029845459966196 0.0 3 0.0 0.0 0.0 0.0980950374133038 0.0 4 0.0 0.0 0.0 0.1417625723495734 0.0 5 0.0 0.0 0.0 0.23716761787768606 0.0 6 0.0 0.0 0.0 0.3301516707091267 0.0 7 0.0 0.0 0.0 0.38960049137185104 0.0 8 0.0 0.0 0.0 0.5345013875874808 0.0 9 0.0 0.0 0.0 0.593860541854032 0.0 10 0.0 0.0 0.0 0.6892655873821447 0.0 11 0.0 0.0 0.0 0.8034109097104224 0.0 12 0.0 0.0 0.0 0.8982779568614968 0.0 13 0.0 0.0 0.0 0.9414971598169012 0.0 14 0.0 0.0 0.0 0.9582647759012594 0.0 15 0.0 0.0 0.0 0.9822953700756336 0.0 16 0.0 0.0 0.0 1.0367228725526678 0.0 17 0.0 0.0 0.0 1.1033450049092353 0.0 18 0.0 0.0 0.0 1.2080753556393438 0.0 19 0.0 0.0 0.0 1.252998220122036 0.0 20 0.0 0.0 0.0 1.2969347542468246 0.0 21 0.0 0.0 0.0 1.356293908513376 0.0 22 0.0 0.0 0.0 1.419688050607714 0.0 23 0.0 0.0 0.0 1.5000291415787563 0.0 24 0.0 0.0 0.0 1.5621679541266718 0.0 25 0.0 0.0 0.0 1.6057458226667682 0.0 26 0.0 0.0 0.0 1.6582006644279956 0.0 27 0.0 0.0 0.0 1.717470152298374 0.0 28 0.0 0.0 0.0 1.7736013163026958 0.0 29 0.0 0.0 0.0 1.8404924478477824 0.0 30 0.0 0.0 0.0 1.9327591695098387 0.0 31 0.0 0.0 0.0 2.022694564871396 0.0 32 0.0 0.0 0.0 2.097566005675883 0.0 33 0.0 0.0 0.0 2.1739617752153113 0.0 34 0.0 0.0 0.0 2.2608485131069855 0.0 35 0.0 0.0 0.0 2.3846778062219514 0.0 36 0.0 0.0 0.0 2.47317853924474 0.0 37 0.0 0.0 0.0 2.5712735766580437 0.0 38 0.0 0.0 0.0 2.6555599890607 0.0 39 0.0 0.0 0.0 2.7510547009849855 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTA 30 2.1654432E-6 45.000004 32 GCGACAG 30 2.1654432E-6 45.000004 1 CACGGGC 30 2.1654432E-6 45.000004 4 CGACGCA 30 2.1654432E-6 45.000004 43 TCCGATG 45 3.8562575E-10 45.000004 1 CGTGCAT 45 3.8562575E-10 45.000004 23 ACACCAT 30 2.1654432E-6 45.000004 39 CAACCGT 30 2.1654432E-6 45.000004 9 AATGCGT 60 0.0 45.000004 22 CTAACGG 45 3.8562575E-10 45.000004 1 ACGGCAT 30 2.1654432E-6 45.000004 18 ACGTGCA 45 3.8562575E-10 45.000004 22 ACGCTAG 30 2.1654432E-6 45.000004 1 CTCGATG 65 0.0 45.000004 1 ATGTTCG 30 2.1654432E-6 45.000004 1 TGATGCG 65 0.0 45.000004 1 ATACCGG 65 0.0 45.000004 2 CTGTGCG 30 2.1654432E-6 45.000004 1 TACGTAG 65 0.0 45.000004 1 TGTCACG 40 6.8139343E-9 45.0 1 >>END_MODULE