##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545112_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1529426
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88259255433084	31.0	31.0	33.0	30.0	33.0
2	31.193344431178755	31.0	31.0	33.0	30.0	34.0
3	31.342519350396817	31.0	31.0	34.0	28.0	34.0
4	35.19917406922597	37.0	35.0	37.0	33.0	37.0
5	35.08816444862321	37.0	35.0	37.0	32.0	37.0
6	35.16698356115301	37.0	35.0	37.0	32.0	37.0
7	35.56365329215013	36.0	35.0	37.0	35.0	37.0
8	35.62662528294929	37.0	35.0	37.0	35.0	37.0
9	37.45590567964714	39.0	37.0	39.0	35.0	39.0
10	36.96966705156052	39.0	37.0	39.0	33.0	39.0
11	36.5419288020473	39.0	35.0	39.0	32.0	39.0
12	35.115373349217286	35.0	35.0	39.0	31.0	39.0
13	34.6921649036959	35.0	33.0	39.0	30.0	39.0
14	35.33580114369705	35.0	33.0	40.0	28.0	41.0
15	35.786109952361215	36.0	34.0	40.0	31.0	41.0
16	35.96995539503055	35.0	34.0	40.0	31.0	41.0
17	35.81036022664712	35.0	34.0	40.0	31.0	41.0
18	35.89008359999111	36.0	35.0	40.0	31.0	41.0
19	35.76522760826611	36.0	35.0	40.0	31.0	41.0
20	35.48605097598707	35.0	34.0	40.0	30.0	41.0
21	35.33704997822713	35.0	34.0	40.0	30.0	41.0
22	35.159805051045296	35.0	34.0	40.0	30.0	41.0
23	35.11771671202137	35.0	34.0	39.0	29.0	41.0
24	35.2059177756884	35.0	34.0	39.0	30.0	41.0
25	35.08371114391935	35.0	34.0	39.0	30.0	41.0
26	34.85027389360453	35.0	34.0	39.0	29.0	41.0
27	34.8236338338697	35.0	34.0	39.0	29.0	41.0
28	34.900147506319364	35.0	34.0	39.0	29.0	41.0
29	34.90845585206476	36.0	34.0	39.0	29.0	41.0
30	34.86280604618988	36.0	34.0	39.0	29.0	41.0
31	34.591483994648975	35.0	34.0	39.0	29.0	41.0
32	34.42685425774114	35.0	34.0	39.0	27.0	41.0
33	34.16796105205482	35.0	33.0	39.0	26.0	41.0
34	33.96174185609503	35.0	33.0	39.0	24.0	41.0
35	33.75184677127236	35.0	33.0	39.0	23.0	41.0
36	33.47472188912703	35.0	33.0	39.0	23.0	41.0
37	33.48640862650432	35.0	33.0	39.0	23.0	41.0
38	33.55285381574525	35.0	33.0	39.0	23.0	41.0
39	33.56182057843923	35.0	33.0	39.0	23.0	41.0
40	33.22592332025217	35.0	32.0	39.0	23.0	41.0
41	33.33776201006129	35.0	32.0	39.0	23.0	41.0
42	33.31739881498026	35.0	33.0	39.0	23.0	41.0
43	33.28401177958267	35.0	33.0	39.0	23.0	41.0
44	33.26105872399188	35.0	33.0	39.0	23.0	41.0
45	33.3291215135613	35.0	33.0	39.0	23.0	41.0
46	33.343604724909866	35.0	33.0	39.0	23.0	41.0
47	33.271371089546015	35.0	33.0	39.0	23.0	41.0
48	33.316760013233726	35.0	33.0	39.0	23.0	41.0
49	33.36713250592052	35.0	33.0	39.0	23.0	41.0
50	33.122908856002184	35.0	32.0	38.0	23.0	41.0
51	32.838947422104766	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	4.0
13	3.0
14	19.0
15	51.0
16	151.0
17	390.0
18	945.0
19	1908.0
20	3376.0
21	5579.0
22	8689.0
23	12655.0
24	17680.0
25	24127.0
26	30740.0
27	35253.0
28	38413.0
29	43565.0
30	50761.0
31	62267.0
32	79790.0
33	106433.0
34	195118.0
35	285520.0
36	84941.0
37	100482.0
38	144411.0
39	196048.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.316977741976405	24.243016661152616	30.400817038549103	14.03918855832188
2	29.97026335370263	26.420696391979735	31.87208795979668	11.73695229452095
3	27.228319644101774	25.705329973467173	34.11999011393817	12.946360268492885
4	25.786013837871202	27.680319283182058	33.33832431251986	13.195342566426882
5	21.303090178929875	33.59855265962525	32.17998124786685	12.918375913578034
6	19.50064926318763	41.419460634251024	30.028782039797935	9.051108062763417
7	86.93751773541184	4.274414061222968	7.616256033309228	1.1718121700559556
8	88.28880900416235	3.139151550974026	7.29469748781569	1.2773419570479383
9	84.60108563604908	4.8195205260012575	8.887124973682937	1.6922688642667247
10	60.56886701285319	19.64436331015688	13.013313491466732	6.773456185523196
11	56.77901382610207	16.007443315335294	19.53052975429998	7.6830131042626455
12	50.74322000541379	21.501007567544946	19.94467205343704	7.811100373604216
13	16.91621562599302	54.350259509123035	19.755058433686887	8.978466431197063
14	9.944580515827507	54.12546929370888	25.802555991594232	10.12739419886938
15	9.104984484375183	22.61462797153965	59.111261349028986	9.169126195056185
16	9.78262433095815	16.71764439730984	56.66236875795233	16.83736251377968
17	10.224750985010063	16.718886693439238	31.379811772521194	41.67655054902951
18	15.772126274824672	22.621231756227502	45.22637904677964	16.380262922168185
19	33.58181435388178	19.473449516354503	28.547049677460695	18.397686452303024
20	33.557622271361936	20.870051901824606	29.08195623717656	16.490369589636895
21	16.402558868490534	35.42211260956725	31.21314793916149	16.96218058278073
22	18.96731191963521	25.002059596214526	21.58025298379915	34.45037550035111
23	12.371700232636297	35.129126875049856	25.72344134335365	26.775731548960195
24	10.307919441672889	20.661411536092626	56.925604769371	12.105064252863492
25	9.586668462547387	25.29654916288856	49.3032680234284	15.813514351135655
26	9.311924865930093	43.35561184392053	29.712650366869664	17.619812923279714
27	9.92522684981163	51.0809284005895	26.828888746497054	12.164956003101818
28	9.18148377234335	35.17777257611679	43.98604443758639	11.65469921395347
29	9.32617857941476	25.00918645295686	42.922900486849315	22.741734480779062
30	14.192317902271833	43.400203736565224	29.813472505371298	12.594005855791652
31	32.91012445191856	30.398855518344792	26.000669532229736	10.690350497506907
32	36.39012283039519	22.57729370365091	29.667339250150054	11.365244215803838
33	36.12577529086076	27.47880577419241	21.058423225445363	15.336995709501473
34	19.20393664028204	26.15556424436357	24.849191788291815	29.791307327062572
35	18.583377031644552	24.529006306941298	35.684040940849705	21.203575720564448
36	38.39263880697726	26.560552782547177	22.889763872197804	12.15704453827776
37	15.330784228854485	46.28180768471309	27.218904347121075	11.16850373931135
38	15.937678580068601	43.99081747008355	23.45173941073318	16.619764539114673
39	19.784154316717515	41.123074931379485	26.082138004715492	13.010632747187506
40	28.162330181388313	24.643886006907167	24.503245008258002	22.690538803446522
41	13.397967603532305	24.563136758496324	27.78820289441921	34.25069274355216
42	15.503594158854368	21.004612187840404	27.09474011818813	36.397053535117095
43	16.841481706208736	21.52317274585367	28.742155553782922	32.89318999415467
44	11.579965294169185	32.14781231651613	35.45225463670684	20.81996775260784
45	11.609126561206622	52.5013305645386	21.987856882255173	13.901685991999614
46	18.15635408316584	43.396346080163404	23.579630528054317	14.867669308616435
47	16.823893408376737	30.37407497976365	25.9237125562139	26.87831905564571
48	17.766796170589487	27.68888458807422	39.698095886953666	14.846223354382623
49	22.396572308826972	27.46925970919809	38.1180913623804	12.01607661959454
50	17.74685404851232	45.216506061751275	24.398892133388607	12.637747756347807
51	15.975862840045874	40.11282664215202	24.392484500721185	19.51882601708092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2192.0
1	2879.5
2	3567.0
3	8172.5
4	12778.0
5	10227.0
6	7676.0
7	8056.0
8	8436.0
9	9008.0
10	9580.0
11	9812.0
12	10044.0
13	9712.0
14	9380.0
15	8883.0
16	8386.0
17	8052.5
18	7719.0
19	7333.0
20	6947.0
21	7606.5
22	8266.0
23	8005.0
24	7744.0
25	9044.0
26	13704.5
27	17065.0
28	21016.0
29	24967.0
30	30366.5
31	35766.0
32	42576.5
33	49387.0
34	54915.0
35	60443.0
36	64818.0
37	69193.0
38	81064.0
39	92935.0
40	134979.0
41	177023.0
42	210322.5
43	243622.0
44	235044.5
45	226467.0
46	187661.5
47	148856.0
48	125794.5
49	102733.0
50	85203.0
51	67673.0
52	52153.0
53	36633.0
54	27558.0
55	18483.0
56	14799.5
57	11116.0
58	10046.5
59	8977.0
60	8159.0
61	7341.0
62	6313.5
63	5286.0
64	4614.0
65	3942.0
66	3273.5
67	2605.0
68	2169.5
69	1734.0
70	1596.5
71	1459.0
72	1323.5
73	1188.0
74	954.0
75	558.5
76	397.0
77	284.0
78	171.0
79	139.5
80	108.0
81	69.5
82	31.0
83	27.0
84	23.0
85	17.5
86	12.0
87	7.5
88	3.0
89	4.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1529426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.27964464971229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41888028096358	13.896096743828053
2	7.495753320686798	2.5904790752673748
3	2.645629462778178	1.3714661097484844
4	1.5140463337225376	1.046487305197006
5	1.0114928731681156	0.8739118707030771
6	0.7145643907315454	0.7408451250707563
7	0.5388638256797158	0.6517962795630998
8	0.42929314354666914	0.5934426376835497
9	0.34510633555217207	0.5366983360235315
>10	3.1405809992933484	11.362346118469572
>50	0.5100271211007809	6.29344999974434
>100	1.154871535572725	49.895330392292315
>500	0.07329503662135348	8.110009077336242
>1k	0.007215573553397493	1.5856995075878573
>5k	3.7976702912618387E-4	0.45194142148475314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6887	0.45029965490321205	No Hit
TTTAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1686	0.11023743548233128	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1614	0.10552978699198261	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1574	0.10291442671956669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018176753893290685	0.0
2	0.0	0.0	0.0	0.06760706304195169	0.0
3	0.0	0.0	0.0	0.09650679405214767	0.0
4	0.0	0.0	0.0	0.15306395994314206	0.0
5	0.0	0.0	0.0	0.27016671614056514	0.0
6	0.0	0.0	0.0	0.40067319373411986	0.0
7	0.0	0.0	0.0	0.4948261635410932	0.0
8	0.0	0.0	0.0	0.7691120721107134	0.0
9	0.0	0.0	0.0	0.8755572351980416	0.0
10	0.0	0.0	0.0	1.0426787566054192	0.0
11	0.0	0.0	0.0	1.17089679396061	0.0
12	0.0	0.0	0.0	1.2917918225530363	0.0
13	0.0	0.0	0.0	1.3427259638583364	0.0
14	0.0	0.0	0.0	1.3610988697720583	0.0
15	0.0	0.0	0.0	1.3888870726664775	0.0
16	0.0	0.0	0.0	1.4570172077629124	0.0
17	0.0	0.0	0.0	1.5318818955608182	0.0
18	0.0	0.0	0.0	1.6598383968887673	0.0
19	0.0	0.0	0.0	1.7126686743915691	0.0
20	0.0	0.0	0.0	1.7662835599760955	0.0
21	0.0	0.0	0.0	1.845202056196246	0.0
22	0.0	0.0	0.0	1.9261474566275192	0.0
23	0.0	0.0	0.0	2.026838827115532	0.0
24	0.0	0.0	0.0	2.1047765632335267	0.0
25	0.0	0.0	0.0	2.1702259540507356	0.0
26	0.0	0.0	0.0	2.2347599687725985	0.0
27	0.0	0.0	0.0	2.3000132075693758	0.0
28	0.0	0.0	0.0	2.3674241185908964	0.0
29	0.0	0.0	0.0	2.4509194952877746	0.0
30	0.0	0.0	0.0	2.5727298999755464	0.0
31	0.0	0.0	0.0	2.684340399600896	0.0
32	0.0	0.0	0.0	2.783854857966322	0.0
33	0.0	0.0	0.0	2.882323172222782	0.0
34	0.0	0.0	0.0	2.9822953186358805	0.0
35	0.0	0.0	0.0	3.125943981598325	0.0
36	0.0	0.0	0.0	3.2469043941975615	0.0
37	0.0	0.0	0.0	3.3598879579659298	0.0
38	0.0	0.0	0.0	3.4764676421088696	0.0
39	0.0	0.0	0.0	3.5851358614277515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAA	20	7.0340186E-4	45.000004	25
ACCGGGT	20	7.0340186E-4	45.000004	4
CGTATTA	20	7.0340186E-4	45.000004	31
CTCGTAG	65	0.0	45.000004	1
CACGGGT	40	6.8175723E-9	45.000004	4
GACACGC	20	7.0340186E-4	45.000004	13
CGCATCA	20	7.0340186E-4	45.000004	22
CCAGCGA	20	7.0340186E-4	45.000004	34
TTCGTGC	20	7.0340186E-4	45.000004	1
ATTCGCA	20	7.0340186E-4	45.000004	43
CTAAGCG	80	0.0	45.000004	1
CGATTAC	20	7.0340186E-4	45.000004	17
ACGGGCT	80	0.0	45.000004	5
TGGTTCG	20	7.0340186E-4	45.000004	37
TACGCGA	20	7.0340186E-4	45.000004	43
CGCTACT	20	7.0340186E-4	45.000004	39
ACGCTTC	65	0.0	45.000004	24
GACGGAC	20	7.0340186E-4	45.000004	29
CGAGAAT	20	7.0340186E-4	45.000004	25
ACGTACT	20	7.0340186E-4	45.000004	19
>>END_MODULE