FastQCFastQC Report
Thu 26 May 2016
SRR1545111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545111_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1469760
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.3435254735467015No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18850.12825223165686916No Hit
ATAGTTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT18710.12729969518833006No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16980.11552906596995428No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16840.1145765295014152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16480.11212715001088613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG453.8562575E-1045.0000041
CCGGGTA453.8562575E-1045.0000045
ACCGGGT453.8562575E-1045.0000044
TGTTGCG900.045.0000041
GCGTAAG1850.045.0000041
AATCGAG453.8562575E-1045.0000041
TCACGAG253.8913382E-545.01
AACGTTG207.03393E-445.01
CGGGTAC207.03393E-445.06
AGTCCAA207.03393E-445.020
CTCGTTG551.8189894E-1245.01
TTAGCGA502.1827873E-1145.013
TATGGGC2550.045.04
TAGCGCG502.1827873E-1145.01
ATAGCCG351.2122291E-745.02
ACCGAGT207.03393E-445.038
AAGCGTG502.1827873E-1145.018
CGCGATT253.8913382E-545.015
TATGCGT351.2122291E-745.011
CCTCGGA406.8175723E-945.027