##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545111_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1469760
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.805486610058786	31.0	31.0	33.0	28.0	33.0
2	31.10901099499238	31.0	31.0	33.0	30.0	34.0
3	31.26940997169606	31.0	31.0	34.0	28.0	34.0
4	35.14142921293272	35.0	35.0	37.0	32.0	37.0
5	35.01683268016547	35.0	35.0	37.0	32.0	37.0
6	35.10550838232092	37.0	35.0	37.0	32.0	37.0
7	35.47967559329415	35.0	35.0	37.0	35.0	37.0
8	35.528671347703025	37.0	35.0	37.0	35.0	37.0
9	37.368954114957546	39.0	37.0	39.0	35.0	39.0
10	36.90292156542565	39.0	37.0	39.0	32.0	39.0
11	36.46118345852384	38.0	35.0	39.0	32.0	39.0
12	34.95905726104942	35.0	34.0	39.0	30.0	39.0
13	34.491372741127805	35.0	33.0	39.0	27.0	39.0
14	35.10751823426954	35.0	33.0	40.0	27.0	41.0
15	35.6029753156978	35.0	33.0	40.0	30.0	41.0
16	35.8306601077727	35.0	34.0	40.0	31.0	41.0
17	35.66842341606793	35.0	34.0	40.0	31.0	41.0
18	35.77495509470934	36.0	35.0	39.0	31.0	41.0
19	35.64569181362943	36.0	34.0	39.0	31.0	41.0
20	35.34202386784237	35.0	34.0	39.0	30.0	41.0
21	35.19666476159373	35.0	34.0	39.0	30.0	41.0
22	35.026926164816025	35.0	34.0	39.0	29.0	41.0
23	34.98321222512519	35.0	34.0	39.0	29.0	41.0
24	35.081927661659044	35.0	34.0	39.0	30.0	41.0
25	34.989035624863924	35.0	34.0	39.0	30.0	41.0
26	34.723241209449164	35.0	34.0	39.0	29.0	41.0
27	34.707254245591116	35.0	34.0	39.0	29.0	41.0
28	34.79272262138036	35.0	34.0	39.0	29.0	41.0
29	34.79371870237318	36.0	34.0	39.0	29.0	41.0
30	34.74173742651862	35.0	34.0	39.0	29.0	41.0
31	34.479585782712824	35.0	34.0	39.0	28.0	41.0
32	34.31456700413673	35.0	34.0	39.0	27.0	41.0
33	34.08426409753974	35.0	33.0	39.0	26.0	41.0
34	33.891927933812326	35.0	33.0	39.0	24.0	41.0
35	33.65894771935554	35.0	33.0	39.0	23.0	41.0
36	33.4110541857174	35.0	33.0	39.0	23.0	41.0
37	33.43026412475506	35.0	33.0	39.0	23.0	41.0
38	33.49818201611147	35.0	33.0	39.0	23.0	41.0
39	33.48747346505552	35.0	33.0	39.0	23.0	41.0
40	33.088845117570216	35.0	32.0	39.0	23.0	41.0
41	33.21229384389288	35.0	32.0	39.0	23.0	41.0
42	33.220507429784455	35.0	32.0	39.0	23.0	41.0
43	33.16878129762682	35.0	32.0	39.0	23.0	41.0
44	33.134385205747876	35.0	32.0	38.0	23.0	41.0
45	33.245012791204005	35.0	33.0	38.0	23.0	41.0
46	33.24517472240366	35.0	33.0	38.0	23.0	41.0
47	33.160712633355104	35.0	32.0	38.0	23.0	41.0
48	33.21346342259961	35.0	33.0	38.0	23.0	41.0
49	33.260697664924884	35.0	32.0	38.0	23.0	40.0
50	33.017430736991074	35.0	32.0	38.0	23.0	40.0
51	32.66828325713042	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	0.0
13	4.0
14	11.0
15	35.0
16	145.0
17	410.0
18	1017.0
19	2047.0
20	3589.0
21	5706.0
22	8835.0
23	12540.0
24	17498.0
25	23482.0
26	29851.0
27	33984.0
28	37947.0
29	42888.0
30	50719.0
31	62680.0
32	79357.0
33	105303.0
34	193154.0
35	274940.0
36	78704.0
37	93783.0
38	132796.0
39	178266.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40964511212715	27.346437513607665	26.15413400827346	14.089783365991726
2	30.369448073154803	26.887314935771826	29.417183757892445	13.326053233180927
3	27.03631885477901	26.955489331591554	33.45240039190072	12.555791421728719
4	24.78030426736338	28.22127422164163	33.61671293272371	13.381708578271281
5	20.72583278902678	34.115842042238185	32.7895030481167	12.368822120618333
6	19.377721532767257	41.36518887437405	29.300361963858045	9.956727629000653
7	86.27823590246027	4.913931526235576	7.564840518179839	1.2429920531243195
8	88.00457217504899	3.4488623993032874	7.060132266492489	1.4864331591552362
9	84.25532059655998	4.927811343348574	8.54479642934901	2.2720716307424342
10	62.11497115175266	19.2322556063575	12.74412148922273	5.908651752667103
11	59.368060091443496	15.649697909862834	17.94388199433921	7.038360004354452
12	51.97794197692141	21.19060254735467	18.93064173742652	7.900813738297409
13	17.22206346614413	55.44803233180927	18.84436914870455	8.485535053342042
14	9.261784236882212	54.895493141737425	25.868305029392552	9.974417591987809
15	7.9312949052906605	21.76566242107555	60.91429893315915	9.388743740474634
16	8.541258436751578	15.994652188112346	57.81957598519486	17.644513389941217
17	9.274983670803397	17.028902677988242	30.839524820378838	42.85658883082952
18	15.01660134988025	22.24594491617679	45.74814935771826	16.98930437622469
19	33.15296374918354	18.401507729153057	28.691691160461573	19.75383736120183
20	32.86699869366427	20.403059002830396	29.723424232527762	17.006518070977574
21	15.59302198998476	35.35733725234052	31.885001632919664	17.164639124755062
22	18.719314718049205	24.14183268016547	22.0746924667973	35.06416013498803
23	11.59094001741781	33.94547409100805	26.09289952101023	28.370686370563902
24	9.761729806226867	19.953257674722405	57.755075658610934	12.5299368604398
25	9.281515349444808	24.962306771173527	49.32111365120836	16.435064228173307
26	8.939350642281733	42.78936697147834	29.533461245373395	18.737821140866536
27	9.403712170694535	52.49129109514479	25.977574569997824	12.127422164162857
28	8.024235249292401	36.06269050729371	43.460224798606575	12.452849444807315
29	8.657467885913347	25.3015458306118	41.58685771826693	24.454128565207924
30	13.381504463313737	44.25239494883518	29.843171674286957	12.522928913564119
31	33.019608643588064	30.239698998475937	25.877013934247767	10.86367842368822
32	36.57515512736773	21.326611147398214	30.96253810145874	11.13569562377531
33	36.33940235140431	27.574093729588505	20.613161332462443	15.473342586544742
34	19.41378184193338	26.139777922926193	24.293285978663185	30.15315425647725
35	18.351227411278032	24.487807533202698	35.514913999564556	21.64605105595471
36	38.70455040278685	26.53004572175049	22.453665904637493	12.311737970825169
37	14.742270846940997	46.248026888743745	28.073903222294796	10.935799042020466
38	15.374142717178316	44.08311561071196	21.724091008055737	18.818650664053997
39	19.41949706074461	41.29789897670368	26.509225996080993	12.773377966470717
40	28.02736501197474	24.166938819943393	24.603880905726104	23.20181526235576
41	12.886593729588505	24.398337143479207	27.89373775310255	34.821331373829736
42	15.626020574787722	20.429389832353582	26.824175375571524	37.12041421728718
43	16.728989767036794	20.87674178097104	29.89821467450468	32.49605377748748
44	10.393465599825822	32.01760831700414	35.65554920531243	21.93337687785761
45	10.637927280644458	54.32261049423035	21.37981711299804	13.65964511212715
46	18.514791530590028	43.28305301545831	22.771745046810363	15.4304104071413
47	17.108235358153713	30.71583115610712	24.630483888526015	27.54544959721315
48	18.126905072937078	27.339973873285434	39.75200032658393	14.781120727193557
49	23.340817548443283	26.44921619856303	38.3367352492924	11.873231003701285
50	19.017866862617026	45.031637818419334	23.477438493359458	12.47305682560418
51	15.718212497278467	39.75778358371434	24.190888308295232	20.333115610711953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2124.0
1	2601.0
2	3078.0
3	6697.5
4	10317.0
5	8356.0
6	6395.0
7	6813.0
8	7231.0
9	7643.0
10	8055.0
11	8191.0
12	8327.0
13	8273.5
14	8220.0
15	7884.0
16	7548.0
17	7239.5
18	6931.0
19	6849.0
20	6767.0
21	7332.5
22	7898.0
23	8367.0
24	8836.0
25	10276.0
26	14234.0
27	16752.0
28	22611.0
29	28470.0
30	33342.0
31	38214.0
32	43361.0
33	48508.0
34	47899.0
35	47290.0
36	55713.5
37	64137.0
38	75990.0
39	87843.0
40	126306.0
41	164769.0
42	196739.5
43	228710.0
44	223622.5
45	218535.0
46	187631.5
47	156728.0
48	128568.0
49	100408.0
50	85056.0
51	69704.0
52	54014.0
53	38324.0
54	27770.0
55	17216.0
56	13736.5
57	10257.0
58	9048.5
59	7840.0
60	7215.0
61	6590.0
62	5827.0
63	5064.0
64	4222.5
65	3381.0
66	2829.5
67	2278.0
68	2004.0
69	1730.0
70	1517.5
71	1305.0
72	1156.0
73	1007.0
74	783.5
75	469.5
76	379.0
77	269.5
78	160.0
79	112.5
80	65.0
81	58.0
82	51.0
83	34.5
84	18.0
85	17.0
86	16.0
87	9.0
88	2.0
89	3.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1469760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.988769764075613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.26767326916968	13.806377904320458
2	7.4533518645787495	2.5324655759594408
3	2.550059992910838	1.2996714631242754
4	1.4090053243093472	0.9574906820419276
5	0.967082104674948	0.8214767609640181
6	0.6350197991290034	0.647292309781931
7	0.48145172825368565	0.5725490794672714
8	0.4052424313206528	0.5507656291472635
9	0.31684898246231924	0.4844586971730575
>10	2.8526035110585086	9.973587813325041
>50	0.4620188929524095	5.656153203858738
>100	1.0594294090881986	46.90648465491296
>500	0.13177582335264662	13.682880956956453
>1k	0.008035111180039427	1.7637342212813347
>5k	4.017555590019714E-4	0.3446110476858271
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5049	0.3435254735467015	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1885	0.12825223165686916	No Hit
ATAGTTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1871	0.12729969518833006	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1698	0.11552906596995428	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1684	0.1145765295014152	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1648	0.11212715001088613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04089102982799913	0.0
2	0.0	0.0	0.0	0.1530862181580666	0.0
3	0.0	0.0	0.0	0.20479534073590247	0.0
4	0.0	0.0	0.0	0.31773895057696494	0.0
5	0.0	0.0	0.0	0.5916612236011322	0.0
6	0.0	0.0	0.0	0.7928505334204223	0.0
7	0.0	0.0	0.0	0.952128238623993	0.0
8	0.0	0.0	0.0	1.3468865665142609	0.0
9	0.0	0.0	0.0	1.477384062704115	0.0
10	0.0	0.0	0.0	1.751306335728282	0.0
11	0.0	0.0	0.0	1.9199052906596996	0.0
12	0.0	0.0	0.0	2.1125898105813192	0.0
13	0.0	0.0	0.0	2.179879708251687	0.0
14	0.0	0.0	0.0	2.2028086218158065	0.0
15	0.0	0.0	0.0	2.248938602220771	0.0
16	0.0	0.0	0.0	2.328883627258872	0.0
17	0.0	0.0	0.0	2.4171973655562815	0.0
18	0.0	0.0	0.0	2.5640240583496627	0.0
19	0.0	0.0	0.0	2.61981548007838	0.0
20	0.0	0.0	0.0	2.677511974744176	0.0
21	0.0	0.0	0.0	2.760382647507076	0.0
22	0.0	0.0	0.0	2.8446140866536034	0.0
23	0.0	0.0	0.0	2.958918462878293	0.0
24	0.0	0.0	0.0	3.040972675811017	0.0
25	0.0	0.0	0.0	3.108126496843022	0.0
26	0.0	0.0	0.0	3.1790904637491835	0.0
27	0.0	0.0	0.0	3.2499183540169825	0.0
28	0.0	0.0	0.0	3.3174123666448945	0.0
29	0.0	0.0	0.0	3.3965409318528197	0.0
30	0.0	0.0	0.0	3.5161522969736554	0.0
31	0.0	0.0	0.0	3.6227003048116697	0.0
32	0.0	0.0	0.0	3.7106738515131723	0.0
33	0.0	0.0	0.0	3.7954495972131506	0.0
34	0.0	0.0	0.0	3.8836272588721967	0.0
35	0.0	0.0	0.0	4.007661114739822	0.0
36	0.0	0.0	0.0	4.114685390812105	0.0
37	0.0	0.0	0.0	4.21823971260614	0.0
38	0.0	0.0	0.0	4.314445895928587	0.0
39	0.0	0.0	0.0	4.409835619420858	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	45	3.8562575E-10	45.000004	1
CCGGGTA	45	3.8562575E-10	45.000004	5
ACCGGGT	45	3.8562575E-10	45.000004	4
TGTTGCG	90	0.0	45.000004	1
GCGTAAG	185	0.0	45.000004	1
AATCGAG	45	3.8562575E-10	45.000004	1
TCACGAG	25	3.8913382E-5	45.0	1
AACGTTG	20	7.03393E-4	45.0	1
CGGGTAC	20	7.03393E-4	45.0	6
AGTCCAA	20	7.03393E-4	45.0	20
CTCGTTG	55	1.8189894E-12	45.0	1
TTAGCGA	50	2.1827873E-11	45.0	13
TATGGGC	255	0.0	45.0	4
TAGCGCG	50	2.1827873E-11	45.0	1
ATAGCCG	35	1.2122291E-7	45.0	2
ACCGAGT	20	7.03393E-4	45.0	38
AAGCGTG	50	2.1827873E-11	45.0	18
CGCGATT	25	3.8913382E-5	45.0	15
TATGCGT	35	1.2122291E-7	45.0	11
CCTCGGA	40	6.8175723E-9	45.0	27
>>END_MODULE