FastQCFastQC Report
Thu 26 May 2016
SRR1545110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545110_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1983434
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117890.5943731931589356No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG38330.19325069551091695No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA30750.1550341478466135No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29260.14752192409729792No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24620.1241281534954024No Hit
AAGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA20250.10209565833801378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19890.10028062441200464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCT207.0345256E-445.019
AAGTACG750.045.01
GATCGCT406.8193913E-945.010
ACGTTAT207.0345256E-445.033
ACGTTAG453.8562575E-1045.01
ATTACCG351.2125201E-745.013
TCGGGCA207.0345256E-445.01
TACGATA253.8918333E-545.034
CTACGAT207.0345256E-445.033
GCGATAA1600.045.09
GCTAGAT253.8918333E-545.023
TAGGCGT207.0345256E-445.015
CGGTATA253.8918333E-545.041
AGTAGCG1550.045.01
TACAGCC207.0345256E-445.043
AAAATCG551.8189894E-1245.027
CCGTTTA207.0345256E-445.018
GTACGTG551.8189894E-1245.01
TACGTAG253.8918333E-545.01
GTTGCGA207.0345256E-445.016