FastQCFastQC Report
Thu 26 May 2016
SRR1545109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545109_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1440471
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55980.38862288793040606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14990.10406318488883151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA750.045.00000443
ACGGGCG650.045.0000045
GACGGGC650.045.0000044
TCACGAA207.0338824E-445.023
CGGGTAC207.0338824E-445.06
AACCGAT207.0338824E-445.039
GGTGTCG207.0338824E-445.020
GTCGACG207.0338824E-445.01
CTCCGCG551.8189894E-1245.01
CCGATCA453.8562575E-1045.09
TTAGCCG207.0338824E-445.01
TAGACGC406.8175723E-945.021
GCGAACG207.0338824E-445.01
GCAACTA453.8562575E-1045.09
GATCCCC351.2122109E-745.044
ACCGACA207.0338824E-445.042
ACTTCGA207.0338824E-445.021
TACGGGT950.045.04
TCGATAT207.0338824E-445.016
ACGGGCC207.0338824E-445.05