##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545109_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1440471 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.90230070581081 31.0 31.0 33.0 30.0 33.0 2 31.226686965582786 31.0 31.0 33.0 30.0 34.0 3 31.365374242174955 31.0 31.0 34.0 30.0 34.0 4 35.227470737001994 37.0 35.0 37.0 33.0 37.0 5 35.09935777950407 37.0 35.0 37.0 32.0 37.0 6 35.16466697351075 37.0 35.0 37.0 32.0 37.0 7 35.570015640717514 36.0 35.0 37.0 35.0 37.0 8 35.64294039935549 37.0 35.0 37.0 35.0 37.0 9 37.50913971888362 39.0 37.0 39.0 35.0 39.0 10 37.02329376988499 39.0 37.0 39.0 33.0 39.0 11 36.56455145573913 39.0 35.0 39.0 32.0 39.0 12 34.88743959441044 35.0 34.0 39.0 30.0 39.0 13 34.33972013320643 35.0 33.0 39.0 27.0 39.0 14 34.92962926709389 35.0 33.0 40.0 27.0 41.0 15 35.46447932655361 35.0 33.0 40.0 30.0 41.0 16 35.717368138615775 35.0 34.0 40.0 31.0 41.0 17 35.581558393053385 35.0 34.0 40.0 31.0 41.0 18 35.72101000297819 36.0 35.0 39.0 31.0 41.0 19 35.556381905640585 36.0 34.0 39.0 31.0 41.0 20 35.248693656449866 35.0 34.0 39.0 30.0 41.0 21 35.06719191153449 35.0 34.0 39.0 30.0 41.0 22 34.8852646113667 35.0 34.0 39.0 29.0 41.0 23 34.85169920116407 35.0 34.0 39.0 29.0 41.0 24 34.964607409659756 35.0 34.0 39.0 30.0 41.0 25 34.862349189952454 35.0 34.0 39.0 30.0 41.0 26 34.640932028482354 35.0 34.0 38.0 29.0 41.0 27 34.650271334862 35.0 34.0 38.0 29.0 41.0 28 34.758031227286075 35.0 34.0 38.0 29.0 41.0 29 34.775215190031595 35.0 34.0 38.0 29.0 41.0 30 34.744936204894096 35.0 34.0 38.0 29.0 41.0 31 34.42929222455711 35.0 34.0 38.0 29.0 41.0 32 34.24369945663606 35.0 34.0 38.0 28.0 41.0 33 34.01337618042987 35.0 33.0 38.0 27.0 41.0 34 33.81982559871042 35.0 33.0 38.0 25.0 41.0 35 33.60532492497246 35.0 33.0 38.0 24.0 41.0 36 33.30351947383877 35.0 33.0 38.0 23.0 41.0 37 33.341731975166454 35.0 33.0 38.0 23.0 41.0 38 33.42936372894699 35.0 33.0 38.0 23.0 41.0 39 33.43566166899576 35.0 33.0 38.0 23.0 41.0 40 33.089296487051804 35.0 32.0 38.0 23.0 41.0 41 33.24257551870187 35.0 32.0 38.0 23.0 41.0 42 33.2057792208243 35.0 33.0 38.0 23.0 41.0 43 33.165434083712896 35.0 33.0 38.0 23.0 41.0 44 33.155944132162325 35.0 33.0 38.0 23.0 41.0 45 33.23033299524947 35.0 33.0 38.0 23.0 40.0 46 33.258566121775445 35.0 33.0 38.0 23.0 40.0 47 33.18313523840466 35.0 33.0 38.0 23.0 40.0 48 33.21285051903162 35.0 33.0 38.0 23.0 40.0 49 33.280653341858326 35.0 33.0 38.0 24.0 40.0 50 33.01561780834186 35.0 32.0 38.0 23.0 40.0 51 32.72697471868576 35.0 32.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 0.0 12 2.0 13 2.0 14 14.0 15 32.0 16 123.0 17 347.0 18 889.0 19 1755.0 20 3166.0 21 5228.0 22 7953.0 23 11515.0 24 16343.0 25 22383.0 26 28370.0 27 32840.0 28 36329.0 29 41337.0 30 49211.0 31 60580.0 32 78220.0 33 104715.0 34 199229.0 35 301261.0 36 70869.0 37 83769.0 38 117912.0 39 165970.0 40 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.631578143537773 25.256114145998083 30.807492826998946 13.304814883465198 2 30.327094401761645 25.87611968585275 31.569951772718785 12.226834139666817 3 27.399163190373148 25.691041333008442 33.996588615806914 12.9132068608115 4 25.09422265356262 27.19665998135332 33.582696215335126 14.126421149748936 5 22.90709080571563 33.13638386333359 31.077543386850554 12.878981944100229 6 19.090144820687122 42.778646706528626 29.44529948884774 8.685908983936503 7 86.5835549622311 4.901591215650992 7.611329905287924 0.9035239168299813 8 89.51669280395093 2.5344488018155173 6.925790244996255 1.0230681492372982 9 86.26004966431118 4.372250465299198 7.8438233050162065 1.523876565373409 10 66.1222613992229 16.5718712837676 11.8375170343589 5.468350282650605 11 63.925132821139755 14.10885745009792 16.069674432876468 5.8963352958858595 12 54.904125109078905 20.68503982377986 17.435963653554985 6.974871413586251 13 16.84039456538868 57.55909004763026 18.060481606363474 7.54003378061759 14 8.64411709781037 58.01060902996311 25.710271154365483 7.635002717861034 15 8.152819459746151 20.16965284271603 63.71291056883478 7.9646171287030425 16 8.004048675745642 14.21736362620282 61.53577545122394 16.2428122468276 17 8.452443679879707 15.16177694656817 31.229299305574358 45.15648006797777 18 14.921022360047512 20.51370697501026 48.449708463412314 16.115562201529915 19 35.82793405767974 16.18463683059222 28.68221574748815 19.30521336423989 20 35.16988540553749 19.00899080925614 29.700910327247133 16.120213457959238 21 16.59353086594593 33.82247889752727 33.41191874046753 16.172071496059274 22 20.10925593087261 23.837619778530772 20.20790422021686 35.84522007037976 23 12.15595454542299 34.42214386822088 25.437374303266086 27.984527283090046 24 10.004852579468798 18.085473431953854 59.44979107528023 12.45988291329711 25 8.318806834708925 22.70056113590624 53.479729893902764 15.500902135482075 26 7.8008512493483035 44.17138560929029 30.315778658508226 17.711984482853175 27 8.539845647708285 53.96054484956656 26.240236700357034 11.259372802368114 28 7.550724728231252 35.627235813841445 45.54371452115315 11.278324936774151 29 7.666589608537763 26.427883657498136 43.398721668121055 22.506805065843047 30 13.206305437596452 45.68963901390587 28.651114808975674 12.452940739522003 31 34.56313941759328 31.407504906381316 24.633470580108867 9.395885095916544 32 38.82139938950524 21.172172157579013 31.21680339277917 8.78962506013658 33 38.839171354369505 27.12487790451873 19.065638947261 14.97031179385076 34 18.623908429951037 27.5603604654311 22.929791713960228 30.88593939065764 35 18.21813837279612 22.78254820819024 36.91188507092472 22.087428348088924 36 41.77668276556765 23.653096799588468 22.85849558928989 11.71172484555399 37 15.156362051023589 46.058476706577224 28.157040301401416 10.628120940997771 38 15.21370440640596 45.03804658337447 21.062971764096606 18.68527724612297 39 18.878616785759657 41.24963293256164 26.616155410278996 13.255594871399701 40 29.416420045943305 22.178162559329554 24.47831299623526 23.927104398491885 41 12.208159692211783 22.281948057267382 27.96363134002698 37.546260910493864 42 15.148864503346473 19.168313697394808 26.939868973412167 38.742952825846544 43 15.894176279841801 18.601415786919695 31.059424313297523 34.44498361994098 44 10.704693117737186 29.94430293980233 37.16937029624338 22.181633646217104 45 10.670329357550411 56.32379964608798 20.330017056920966 12.675853939440639 46 19.44287667020023 44.616240104799054 21.825430709816445 14.115452515184272 47 17.505107704355034 30.430324525797463 23.43601502564092 28.62855274420658 48 18.15177119150611 27.75175619641076 40.75403114675686 13.34244146532627 49 23.57548329678279 25.824886443392476 38.84333665863457 11.756293601190167 50 20.128416330491902 45.5600980512624 21.961775002759516 12.349710615486185 51 15.855369528438962 41.42381207257904 22.948188474464253 19.772629924517744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1851.0 1 2438.0 2 3025.0 3 6973.0 4 10921.0 5 8714.0 6 6507.0 7 6999.5 8 7492.0 9 7908.5 10 8325.0 11 8336.5 12 8348.0 13 8167.0 14 7986.0 15 7807.0 16 7628.0 17 6940.5 18 6253.0 19 6140.5 20 6028.0 21 6181.5 22 6335.0 23 6867.0 24 7399.0 25 8101.0 26 11010.0 27 13217.0 28 17677.5 29 22138.0 30 26869.5 31 31601.0 32 36083.5 33 40566.0 34 44535.5 35 48505.0 36 50898.0 37 53291.0 38 63206.5 39 73122.0 40 124133.0 41 175144.0 42 214787.0 43 254430.0 44 239834.0 45 225238.0 46 192070.5 47 158903.0 48 129654.0 49 100405.0 50 81764.0 51 63123.0 52 46998.0 53 30873.0 54 23114.0 55 15355.0 56 12177.0 57 8999.0 58 8186.0 59 7373.0 60 6733.0 61 6093.0 62 5395.5 63 4698.0 64 4083.0 65 3468.0 66 2840.5 67 2213.0 68 1983.0 69 1753.0 70 1436.5 71 1120.0 72 997.5 73 875.0 74 703.5 75 403.0 76 274.0 77 198.5 78 123.0 79 101.5 80 80.0 81 56.0 82 32.0 83 22.5 84 13.0 85 10.0 86 7.0 87 5.5 88 4.0 89 3.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1440471.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.873580525319294 #Duplication Level Percentage of deduplicated Percentage of total 1 81.03678094950165 12.86343867914575 2 7.417999307886855 2.355004187010096 3 2.581623806240662 1.2293884012332732 4 1.3601912378503536 0.8636442057540528 5 0.8689533648520813 0.6896700604863334 6 0.658596898981811 0.6272574545828012 7 0.4719539625551428 0.5244119460203811 8 0.41133300516070825 0.5223462064112063 9 0.3105153496231498 0.4436091365931668 >10 3.0352979487914986 10.090690040721157 >50 0.49951389171342014 5.650526832089339 >100 1.2332160346619678 51.598501158555464 >500 0.10656881096118712 10.815702445342556 >1k 0.007016876441888864 1.336108138418056 >5k 4.38554777618054E-4 0.3897011076363402 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5598 0.38862288793040606 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1499 0.10406318488883151 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.022909173457848164 0.0 2 0.0 0.0 0.0 0.08025152884021962 0.0 3 0.0 0.0 0.0 0.10843675436714796 0.0 4 0.0 0.0 0.0 0.16071132289369242 0.0 5 0.0 0.0 0.0 0.28060266398976447 0.0 6 0.0 0.0 0.0 0.3911914922271951 0.0 7 0.0 0.0 0.0 0.47331740798669325 0.0 8 0.0 0.0 0.0 0.6767925213350355 0.0 9 0.0 0.0 0.0 0.7494770807603902 0.0 10 0.0 0.0 0.0 0.8790180434038589 0.0 11 0.0 0.0 0.0 0.9954382976123781 0.0 12 0.0 0.0 0.0 1.098668421648197 0.0 13 0.0 0.0 0.0 1.1418487425293533 0.0 14 0.0 0.0 0.0 1.15545540312856 0.0 15 0.0 0.0 0.0 1.1821827721627163 0.0 16 0.0 0.0 0.0 1.243968118761155 0.0 17 0.0 0.0 0.0 1.318110534679282 0.0 18 0.0 0.0 0.0 1.4443886756484512 0.0 19 0.0 0.0 0.0 1.4911789268926623 0.0 20 0.0 0.0 0.0 1.5433146519437044 0.0 21 0.0 0.0 0.0 1.6188455026168524 0.0 22 0.0 0.0 0.0 1.6982639706040594 0.0 23 0.0 0.0 0.0 1.7989949120808402 0.0 24 0.0 0.0 0.0 1.8651538281575957 0.0 25 0.0 0.0 0.0 1.9230515574419755 0.0 26 0.0 0.0 0.0 1.9921956082420265 0.0 27 0.0 0.0 0.0 2.048427215820381 0.0 28 0.0 0.0 0.0 2.113336540617617 0.0 29 0.0 0.0 0.0 2.184632665287951 0.0 30 0.0 0.0 0.0 2.2925140457530904 0.0 31 0.0 0.0 0.0 2.4000483175294747 0.0 32 0.0 0.0 0.0 2.4837709332572473 0.0 33 0.0 0.0 0.0 2.5714505880368295 0.0 34 0.0 0.0 0.0 2.6603798340959313 0.0 35 0.0 0.0 0.0 2.802833239961096 0.0 36 0.0 0.0 0.0 2.9069658465876786 0.0 37 0.0 0.0 0.0 3.016235661807839 0.0 38 0.0 0.0 0.0 3.1114128642645356 0.0 39 0.0 0.0 0.0 3.209158671018021 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGTA 75 0.0 45.000004 43 ACGGGCG 65 0.0 45.000004 5 GACGGGC 65 0.0 45.000004 4 TCACGAA 20 7.0338824E-4 45.0 23 CGGGTAC 20 7.0338824E-4 45.0 6 AACCGAT 20 7.0338824E-4 45.0 39 GGTGTCG 20 7.0338824E-4 45.0 20 GTCGACG 20 7.0338824E-4 45.0 1 CTCCGCG 55 1.8189894E-12 45.0 1 CCGATCA 45 3.8562575E-10 45.0 9 TTAGCCG 20 7.0338824E-4 45.0 1 TAGACGC 40 6.8175723E-9 45.0 21 GCGAACG 20 7.0338824E-4 45.0 1 GCAACTA 45 3.8562575E-10 45.0 9 GATCCCC 35 1.2122109E-7 45.0 44 ACCGACA 20 7.0338824E-4 45.0 42 ACTTCGA 20 7.0338824E-4 45.0 21 TACGGGT 95 0.0 45.0 4 TCGATAT 20 7.0338824E-4 45.0 16 ACGGGCC 20 7.0338824E-4 45.0 5 >>END_MODULE