FastQCFastQC Report
Thu 26 May 2016
SRR1545108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545108_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1620678
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93920.5795105505226825No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG53460.3298619466667654No Hit
GAATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC40250.24835284985666495No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC25250.1557989927672246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21870.13494352363640402No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17920.11057100793618474No Hit
ATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA16680.10291988908345767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC351.2123382E-745.00000421
CGAACCC351.2123382E-745.00000434
GTTTACG1250.045.0000041
ATTACCG900.045.0000041
ATACTCC700.045.00000413
CGTTAGT453.8562575E-1045.00000439
ATATCCG351.2123382E-745.0000041
ACGGCTA453.8562575E-1045.00000430
TACACGA351.2123382E-745.00000421
GGTAGCG351.2123382E-745.0000041
GTTGCGT351.2123382E-745.00000418
AACCGCT406.8175723E-945.031
AATCCGT253.891517E-545.016
ATCTCGG207.0341444E-445.045
CAACGAA253.891517E-545.015
ACGCAAC253.891517E-545.022
CGGCCCA1000.045.031
CGTTGTA253.891517E-545.032
ATCCGAT253.891517E-545.036
AACGGAC207.0341444E-445.05