##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545108_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1620678
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.820916307866213	31.0	31.0	33.0	28.0	33.0
2	31.11737063130369	31.0	31.0	33.0	30.0	34.0
3	31.232486033622965	31.0	31.0	34.0	28.0	34.0
4	35.12519636843346	35.0	35.0	37.0	32.0	37.0
5	34.99109570192228	35.0	35.0	37.0	32.0	37.0
6	35.06373382004322	37.0	35.0	37.0	32.0	37.0
7	35.451623949976494	35.0	35.0	37.0	35.0	37.0
8	35.49894858818347	37.0	35.0	37.0	35.0	37.0
9	37.28876803411905	39.0	37.0	39.0	35.0	39.0
10	36.842675102642225	39.0	37.0	39.0	32.0	39.0
11	36.45982298766319	38.0	35.0	39.0	32.0	39.0
12	35.13939166200812	35.0	35.0	39.0	30.0	39.0
13	34.646117859315666	35.0	33.0	39.0	27.0	39.0
14	35.344084389372846	36.0	33.0	40.0	27.0	41.0
15	35.76156398741761	36.0	34.0	40.0	30.0	41.0
16	35.97588478402249	36.0	34.0	40.0	31.0	41.0
17	35.74468278091021	36.0	34.0	40.0	31.0	41.0
18	35.82135254504596	36.0	35.0	40.0	31.0	41.0
19	35.66861585089697	36.0	34.0	40.0	30.0	41.0
20	35.300198435469596	35.0	34.0	39.0	29.0	41.0
21	35.21187120452058	35.0	34.0	40.0	29.0	41.0
22	35.0334921557521	35.0	34.0	39.0	29.0	41.0
23	34.89474220048646	35.0	34.0	39.0	29.0	41.0
24	35.012323237558604	35.0	34.0	39.0	29.0	41.0
25	34.99112346807941	35.0	34.0	39.0	29.0	41.0
26	34.67186140615224	35.0	34.0	39.0	29.0	41.0
27	34.61990413888508	35.0	34.0	39.0	29.0	41.0
28	34.68061761805861	35.0	34.0	39.0	29.0	41.0
29	34.6472531866293	35.0	34.0	39.0	28.0	41.0
30	34.62576279803884	35.0	34.0	39.0	28.0	41.0
31	34.38070671657171	35.0	33.0	39.0	27.0	41.0
32	34.15671157379813	35.0	33.0	39.0	25.0	41.0
33	33.862354520762295	35.0	33.0	39.0	24.0	41.0
34	33.55754628618393	35.0	33.0	39.0	23.0	41.0
35	33.26939651183024	35.0	33.0	39.0	23.0	41.0
36	33.04261179580398	35.0	32.0	39.0	21.0	41.0
37	33.06264106750385	35.0	32.0	39.0	21.0	41.0
38	33.06419041907152	35.0	32.0	39.0	22.0	41.0
39	33.06190063664713	35.0	32.0	39.0	21.0	41.0
40	32.728390833959615	35.0	31.0	39.0	18.0	41.0
41	32.79739837277979	35.0	32.0	39.0	19.0	41.0
42	32.75293118065402	35.0	32.0	39.0	18.0	41.0
43	32.70758842904019	35.0	32.0	39.0	18.0	41.0
44	32.710643323349856	35.0	32.0	39.0	18.0	41.0
45	32.810769320000645	35.0	32.0	39.0	20.0	41.0
46	32.7964419829232	35.0	32.0	39.0	20.0	41.0
47	32.67336941699708	35.0	32.0	38.0	19.0	41.0
48	32.7622988650429	35.0	32.0	38.0	20.0	41.0
49	32.780994127149256	35.0	32.0	38.0	20.0	41.0
50	32.53958405062573	35.0	31.0	38.0	18.0	40.0
51	32.309581545501324	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	9.0
14	16.0
15	53.0
16	195.0
17	568.0
18	1335.0
19	2744.0
20	5080.0
21	8452.0
22	12998.0
23	18070.0
24	23687.0
25	30616.0
26	37259.0
27	41120.0
28	43202.0
29	48072.0
30	56340.0
31	67878.0
32	85062.0
33	111197.0
34	197208.0
35	281844.0
36	90297.0
37	107320.0
38	148811.0
39	201123.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53719862921567	22.840811067960445	28.691387184869544	14.93060311795434
2	30.67623550143829	27.514657445834395	29.17815877058861	12.63094828213871
3	25.949571722451964	26.80952045995565	34.858991113595664	12.381916703996723
4	24.397566944204833	27.768255014259463	35.223036284814135	12.611141756721569
5	24.143537457780013	31.014057079814744	32.03344526179784	12.8089602006074
6	19.539106472723144	41.77609617703208	29.606683128912714	9.07811422133206
7	82.6131409200347	5.06485557279114	10.76191569207455	1.5600878150996065
8	84.66166629028098	5.081206754210275	8.713390321828273	1.543736633680472
9	80.01490734124854	5.413907019161116	10.585014419890934	3.9861712196994095
10	57.676602014712365	19.78431249143877	14.913820018535453	7.625265475313419
11	54.428208441158574	15.893595149684266	21.177062932920666	8.50113347623649
12	48.060441370833686	20.75032794916695	21.98215808445601	9.207072595543346
13	18.35929160511835	49.42709162461637	22.365145945092117	9.848470825173168
14	10.236888512091854	51.1648211427563	27.77485718939851	10.823433155753333
15	9.07792911361788	22.1575784949262	57.43571517599424	11.328777215461677
16	9.601043513887397	16.781310044314786	55.266129360674974	18.35151708112284
17	10.111015266450215	17.839324035989875	33.11280834317489	38.93685235438502
18	15.583416323291857	22.188923401193822	44.52074995773374	17.706910317780583
19	31.45486024984605	19.131375880958462	29.860589210194743	19.55317465900074
20	32.18264207942602	20.66999120121332	31.043180693512223	16.104186025848442
21	16.70449034293055	34.68048557455584	31.93009345471463	16.684930627798984
22	18.914676450226388	24.24435945943611	24.27224902170573	32.56871506863177
23	13.265991146915058	33.458342742975475	27.436727098165086	25.838939011944383
24	11.03828150934362	21.44287761048154	54.641946148463795	12.87689473171105
25	9.734259365524798	26.049900103536917	47.95190654775347	16.263933983184813
26	9.481093715099483	39.88195064041099	31.994017318677738	18.64293832581179
27	11.216046617526738	48.26214707671728	27.433703672166832	13.088102633589152
28	9.437346591981873	34.862693267879244	42.98404741719206	12.715912722946815
29	10.0471531050585	25.12294237350047	42.20795247421141	22.621952047229616
30	14.215902233509677	42.60698300340968	29.734345749124746	13.442769013955887
31	30.381297210179937	29.98405605555206	27.27290677111678	12.361739963151225
32	35.40413333185247	21.343721578253053	31.119259964039742	12.132885125854735
33	34.87065289958894	27.0378199741096	21.794026944278876	16.29750018202259
34	19.167903803223098	25.72491266001019	25.899284126766698	29.20789941000001
35	19.203444484345443	24.126199035218594	35.504893630937175	21.16546284949879
36	36.61226967972663	25.84011136080085	23.812318054542605	13.735300904929911
37	15.508756211906375	42.837565512705176	29.555778507513526	12.097899767874926
38	16.3411856025688	41.500841006048084	23.776222050277724	18.38175134110539
39	19.642273172092175	38.97720583607601	26.86826130792175	14.512259683910067
40	27.514040420120466	23.469683675597498	26.230441827432717	22.78583407684932
41	14.094039655008583	23.9324529610447	28.753336566548075	33.22017081739864
42	15.48969011734595	21.505999341016537	28.337214425073952	34.66709611656356
43	17.23500905176722	21.42449024420644	29.866080739048716	31.474419964977617
44	12.78187277176589	30.7734170513822	34.69486227369039	21.749847903161516
45	11.92704534768782	50.67897509560814	22.707595216323046	14.686384340381
46	19.0013685630335	41.36935282640969	24.11021806922782	15.519060541328999
47	16.78655476288319	30.35556723790907	26.911514810468212	25.94636318873953
48	18.378851320249918	27.021160279833502	39.25184398134608	15.3481444185705
49	22.052930933843736	26.874678375346615	37.56495738203394	13.50743330877571
50	18.69384294721098	42.04135553145042	25.263192318276673	14.00160920306193
51	15.713423641216826	38.74798078335117	25.428493507038414	20.110102068393598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2834.0
1	3711.5
2	4589.0
3	11848.0
4	19107.0
5	14898.0
6	10689.0
7	11109.5
8	11530.0
9	11964.0
10	12398.0
11	12125.0
12	11852.0
13	11432.5
14	11013.0
15	10696.5
16	10380.0
17	9227.0
18	8074.0
19	8835.0
20	9596.0
21	9079.0
22	8562.0
23	8556.5
24	8551.0
25	11297.5
26	16224.0
27	18404.0
28	23578.0
29	28752.0
30	33607.5
31	38463.0
32	45355.0
33	52247.0
34	58093.0
35	63939.0
36	62150.5
37	60362.0
38	76660.5
39	92959.0
40	130264.0
41	167569.0
42	200934.0
43	234299.0
44	225699.0
45	217099.0
46	195220.0
47	173341.0
48	139141.5
49	104942.0
50	87457.0
51	69972.0
52	56388.0
53	42804.0
54	34377.5
55	25951.0
56	22560.5
57	19170.0
58	17850.5
59	16531.0
60	15271.0
61	14011.0
62	12268.0
63	10525.0
64	9044.5
65	7564.0
66	6410.5
67	5257.0
68	4769.5
69	4282.0
70	3760.5
71	3239.0
72	2935.5
73	2632.0
74	2081.0
75	1173.0
76	816.0
77	617.0
78	418.0
79	306.5
80	195.0
81	156.5
82	118.0
83	73.5
84	29.0
85	22.5
86	16.0
87	13.0
88	10.0
89	8.5
90	7.0
91	5.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1620678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.098757160320385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0648763145811	15.482383869635925
2	6.808562677839612	2.600701703897589
3	2.6382507894279645	1.5116193346592477
4	1.5100920211949291	1.1536352321015737
5	1.0424938254635059	0.9955168206830465
6	0.7345971262110625	0.8417935274504585
7	0.6038002504181709	0.8072284049254099
8	0.4930551015373313	0.7533391720734882
9	0.4019510082770624	0.6909088227686591
>10	3.156906812089047	11.882621690834219
>50	0.4786049758537224	6.562644560354884
>100	0.9922297377342885	46.02607678071827
>500	0.0684184557718732	8.161967359318208
>1k	0.005512387431857082	1.6168492265797623
>5k	6.485161684537744E-4	0.9127134939992544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9392	0.5795105505226825	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG	5346	0.3298619466667654	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC	4025	0.24835284985666495	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC	2525	0.1557989927672246	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2187	0.13494352363640402	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1792	0.11057100793618474	No Hit
ATTTGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1668	0.10291988908345767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0214107922733572	0.0
2	0.0	0.0	0.0	0.11501359307647786	0.0
3	0.0	0.0	0.0	0.15400961819682873	0.0
4	0.0	0.0	0.0	0.31221501124837875	0.0
5	0.0	0.0	0.0	0.7365435947177663	0.0
6	0.0	0.0	0.0	0.9118406000451663	0.0
7	0.0	0.0	0.0	1.098182365651906	0.0
8	0.0	0.0	0.0	1.4509359663054597	0.0
9	0.0	0.0	0.0	1.5704538470936238	0.0
10	0.0	0.0	0.0	2.1062789770700903	0.0
11	0.0	0.0	0.0	2.2506629941296175	0.0
12	0.0	0.0	0.0	2.559916281951134	0.0
13	0.0	0.0	0.0	2.6349466087649738	0.0
14	0.0	0.0	0.0	2.6651191661761313	0.0
15	0.0	0.0	0.0	2.730462189281276	0.0
16	0.0	0.0	0.0	2.8118478809485907	0.0
17	0.0	0.0	0.0	2.891752710902474	0.0
18	0.0	0.0	0.0	3.0189217105433652	0.0
19	0.0	0.0	0.0	3.095247791356457	0.0
20	0.0	0.0	0.0	3.1505332953245495	0.0
21	0.0	0.0	0.0	3.2281551301368934	0.0
22	0.0	0.0	0.0	3.312070627231319	0.0
23	0.0	0.0	0.0	3.403698945749865	0.0
24	0.0	0.0	0.0	3.478235651992561	0.0
25	0.0	0.0	0.0	3.5400616285283073	0.0
26	0.0	0.0	0.0	3.6096004264881736	0.0
27	0.0	0.0	0.0	3.6776583627346087	0.0
28	0.0	0.0	0.0	3.7462099195521876	0.0
29	0.0	0.0	0.0	3.8237083492217456	0.0
30	0.0	0.0	0.0	3.9414368554395134	0.0
31	0.0	0.0	0.0	4.040963103096359	0.0
32	0.0	0.0	0.0	4.134565903899479	0.0
33	0.0	0.0	0.0	4.221258016706588	0.0
34	0.0	0.0	0.0	4.312454417225384	0.0
35	0.0	0.0	0.0	4.436414883153841	0.0
36	0.0	0.0	0.0	4.536373048810436	0.0
37	0.0	0.0	0.0	4.646265328461299	0.0
38	0.0	0.0	0.0	4.743385175833818	0.0
39	0.0	0.0	0.0	4.841924182348375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	35	1.2123382E-7	45.000004	21
CGAACCC	35	1.2123382E-7	45.000004	34
GTTTACG	125	0.0	45.000004	1
ATTACCG	90	0.0	45.000004	1
ATACTCC	70	0.0	45.000004	13
CGTTAGT	45	3.8562575E-10	45.000004	39
ATATCCG	35	1.2123382E-7	45.000004	1
ACGGCTA	45	3.8562575E-10	45.000004	30
TACACGA	35	1.2123382E-7	45.000004	21
GGTAGCG	35	1.2123382E-7	45.000004	1
GTTGCGT	35	1.2123382E-7	45.000004	18
AACCGCT	40	6.8175723E-9	45.0	31
AATCCGT	25	3.891517E-5	45.0	16
ATCTCGG	20	7.0341444E-4	45.0	45
CAACGAA	25	3.891517E-5	45.0	15
ACGCAAC	25	3.891517E-5	45.0	22
CGGCCCA	100	0.0	45.0	31
CGTTGTA	25	3.891517E-5	45.0	32
ATCCGAT	25	3.891517E-5	45.0	36
AACGGAC	20	7.0341444E-4	45.0	5
>>END_MODULE