FastQCFastQC Report
Thu 26 May 2016
SRR1545106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545106_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1208439
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.4001029427219744No Hit
TTTTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA15110.12503734156213098No Hit
TTTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA13110.10848706471737506No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13010.10765955087513727No Hit
AAGTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12710.10517700934842389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT800.045.00000428
TCACGAC406.8157533E-945.00000425
CTCACGA406.8157533E-945.00000424
ACAGTCG551.8189894E-1245.0000041
ACGTTCG551.8189894E-1245.0000042
ACGTTCC207.03343E-445.00000412
TAGGTCG207.03343E-445.0000041
GTTAGAC207.03343E-445.00000439
TACGGAG207.03343E-445.0000044
TCGATAG207.03343E-445.0000041
AACGGTC207.03343E-445.00000442
AACGATT207.03343E-445.00000433
TCCAATC207.03343E-445.00000430
ACGTCAC406.8157533E-945.00000434
TCATACG406.8157533E-945.0000042
CACGTTC207.03343E-445.00000411
AGTAGCG1750.045.0000041
CGCGTAG207.03343E-445.00000413
CACAACG207.03343E-445.0000041
TGTAACG551.8189894E-1245.0000041