##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545106_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1208439
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.023957353246626	31.0	31.0	33.0	30.0	33.0
2	31.344876323918708	31.0	31.0	33.0	30.0	34.0
3	31.50074683124262	31.0	31.0	34.0	30.0	34.0
4	35.36944520989475	37.0	35.0	37.0	33.0	37.0
5	35.27220736834875	37.0	35.0	37.0	33.0	37.0
6	35.35241745756302	37.0	35.0	37.0	33.0	37.0
7	35.66876441425674	37.0	35.0	37.0	35.0	37.0
8	35.74101878539173	37.0	35.0	37.0	35.0	37.0
9	37.594512424706586	39.0	37.0	39.0	35.0	39.0
10	37.13027964175271	39.0	37.0	39.0	33.0	39.0
11	36.70399664360386	39.0	35.0	39.0	32.0	39.0
12	35.37215366270039	35.0	35.0	39.0	31.0	39.0
13	34.92491304898303	35.0	35.0	39.0	30.0	39.0
14	35.54261654911833	36.0	34.0	40.0	30.0	41.0
15	35.9412912029486	36.0	35.0	40.0	31.0	41.0
16	36.144746238742705	35.0	35.0	40.0	32.0	41.0
17	36.0178519561186	35.0	35.0	40.0	32.0	41.0
18	36.11938624953349	36.0	35.0	40.0	32.0	41.0
19	35.98658600061732	36.0	35.0	40.0	31.0	41.0
20	35.707593018762225	35.0	34.0	40.0	31.0	41.0
21	35.57047066504805	35.0	34.0	40.0	31.0	41.0
22	35.4147697980618	35.0	34.0	40.0	30.0	41.0
23	35.349158708052286	35.0	34.0	40.0	30.0	41.0
24	35.44501708402327	35.0	34.0	40.0	31.0	41.0
25	35.323061404009636	35.0	34.0	39.0	31.0	41.0
26	35.11541501060459	35.0	34.0	39.0	30.0	41.0
27	35.100173033144415	35.0	34.0	39.0	30.0	41.0
28	35.22192183469749	36.0	34.0	39.0	30.0	41.0
29	35.22118286483637	36.0	34.0	39.0	30.0	41.0
30	35.19819618532669	36.0	34.0	39.0	30.0	41.0
31	34.93759966369837	35.0	34.0	39.0	29.0	41.0
32	34.76950512189693	35.0	34.0	39.0	29.0	41.0
33	34.5445595516199	35.0	34.0	40.0	27.0	41.0
34	34.35511846274409	35.0	34.0	39.0	27.0	41.0
35	34.144800854656296	35.0	33.0	39.0	26.0	41.0
36	33.87742947720158	35.0	33.0	39.0	23.0	41.0
37	33.862932262199415	35.0	33.0	39.0	24.0	41.0
38	33.90289207812724	35.0	33.0	39.0	25.0	41.0
39	33.8974859301959	35.0	33.0	39.0	24.0	41.0
40	33.56394985597122	35.0	33.0	39.0	23.0	41.0
41	33.645709878611996	35.0	33.0	39.0	23.0	41.0
42	33.650894252833616	35.0	33.0	39.0	23.0	41.0
43	33.6055663546112	35.0	33.0	39.0	23.0	41.0
44	33.563715669553865	35.0	33.0	39.0	23.0	41.0
45	33.60174075811853	35.0	33.0	39.0	23.0	41.0
46	33.63874965968493	35.0	33.0	39.0	23.0	41.0
47	33.59468289255808	35.0	33.0	39.0	23.0	41.0
48	33.639520075072056	35.0	33.0	39.0	23.0	41.0
49	33.696467922667175	35.0	33.0	39.0	24.0	41.0
50	33.44978108121303	35.0	33.0	39.0	24.0	41.0
51	33.15606166302147	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	2.0
14	9.0
15	33.0
16	82.0
17	190.0
18	542.0
19	1100.0
20	2049.0
21	3360.0
22	5366.0
23	8142.0
24	11707.0
25	16507.0
26	21388.0
27	24776.0
28	27323.0
29	30886.0
30	36800.0
31	46139.0
32	60356.0
33	82866.0
34	157456.0
35	237412.0
36	67578.0
37	83027.0
38	119594.0
39	163689.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.90095735076409	24.647499791052756	28.896535116791167	13.555007741391995
2	30.60353067055929	24.89111986620756	32.64922763995535	11.8561218232778
3	26.66299250520713	25.640681904506557	35.11836344242448	12.577962147861829
4	24.805554934920174	29.541168399894406	33.12993043091128	12.523346234274133
5	22.19632103896018	32.44557648338063	32.70971890182293	12.648383575836263
6	19.472807481387143	42.01776010208211	28.874688751356086	9.634743665174659
7	86.56407150050602	4.755556548572167	7.5722481647811755	1.1081237861406328
8	89.36892966877103	2.7422153704076084	6.7621121132303745	1.126742847590983
9	85.70668440856345	4.507964406974618	8.135288583039772	1.6500626014221653
10	60.8768833180657	20.495283584856168	12.67916709076751	5.948666006310621
11	55.50747700132154	16.476462610028307	19.96732975350845	8.048730635141698
12	51.61079706960798	19.318476149809797	20.910199025354196	8.160527755228026
13	17.645987923262986	52.53984686028835	20.361309093797868	9.452856122650791
14	10.100385704201868	52.859846463081716	26.86440937440781	10.175358458308612
15	8.823780099781619	22.074511001382778	58.88679527886803	10.214913619967577
16	9.461379515225842	15.922276589881657	58.382342840639865	16.234001054252637
17	9.892183221494838	16.106232917011116	31.74417575069987	42.257408110794174
18	15.141765533882968	21.31882536065122	46.56089384735183	16.97851525811398
19	33.1508665311199	19.474379757687394	29.154140175879796	18.220613535312914
20	34.30102802044621	18.983167540935042	30.051165180865564	16.664639257753183
21	16.510721683096953	34.620862120471116	32.481904341054864	16.386511855377062
22	18.917380190477136	24.152977518931447	22.46344250723454	34.46619978335688
23	12.047691277755849	34.175990678884084	26.290942281737017	27.485375761623054
24	10.591432418185775	19.849409030989566	56.356754457610194	13.20240409321447
25	10.030212530380101	24.06699883072294	50.83574760496806	15.067041033928897
26	9.397081689683965	41.36137612241909	31.191065498548127	18.05047668934882
27	9.837650059291366	49.84736507179924	27.31515616427474	12.999828704634655
28	8.797547910982681	35.16222167606309	43.47021239797789	12.570018014976345
29	9.62886831689477	23.881718481445898	43.18074805596311	23.308665145696224
30	13.508004955152888	42.992157651317115	30.146991283796698	13.352846109733301
31	32.10993686896897	30.4671563893585	26.720670220011105	10.702236521661417
32	36.34953853690588	21.08786624728265	30.908386770039698	11.654208445771777
33	37.176638622222555	26.02233128854663	22.054650669169067	14.74637942006175
34	19.59461751896455	25.986499939177733	24.8815207056376	29.537361836220118
35	17.656414597675184	23.857803331405226	37.54769582908198	20.93808624183761
36	38.61163037604712	24.535123411276864	24.32013531506348	12.53311089761254
37	16.49466791455754	44.51594164041379	27.934964032110848	11.054426412917822
38	16.107556939158698	42.56094018812699	23.861278889542625	17.470223983171678
39	18.58488512866599	41.03268762428223	27.101657592977386	13.28076965407439
40	29.3671422388718	23.778279251166172	25.346169728054125	21.5084087819079
41	13.915721025223448	23.485422102398218	28.24635749094493	34.352499381433404
42	16.2279602032043	20.04850886143198	26.867140170087193	36.85639076527652
43	16.661411953768457	20.278061201268745	29.91992148548665	33.14060535947615
44	12.019307552967092	29.401318560556224	37.05491133602937	21.524462550447314
45	11.149921510312064	51.79193984967384	22.765485059651336	14.292653580362765
46	18.163597831582727	43.23561222370348	24.434497728060748	14.166292216653053
47	17.413456533594164	29.767162430209552	24.987773482980938	27.83160755321535
48	17.859155489023443	25.962336534984388	41.40175879792029	14.776749178071876
49	22.33534336445613	24.95086636561713	40.40816292754537	12.30562734238137
50	18.766938173958305	43.85873014690853	25.01739847853305	12.356933200600114
51	15.831167315851276	40.2352952859019	24.694337074523414	19.239200323723416
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1801.0
1	2399.0
2	2997.0
3	6720.5
4	10444.0
5	8359.5
6	6275.0
7	6521.0
8	6767.0
9	6924.0
10	7081.0
11	7030.5
12	6980.0
13	6658.5
14	6337.0
15	6026.0
16	5715.0
17	5147.5
18	4580.0
19	5067.0
20	5554.0
21	5243.5
22	4933.0
23	6440.0
24	7947.0
25	9295.0
26	11851.0
27	13059.0
28	17338.0
29	21617.0
30	24933.0
31	28249.0
32	34867.0
33	41485.0
34	45497.5
35	49510.0
36	50834.5
37	52159.0
38	64711.5
39	77264.0
40	101772.0
41	126280.0
42	156294.0
43	186308.0
44	181007.0
45	175706.0
46	147398.5
47	119091.0
48	99225.5
49	79360.0
50	68600.0
51	57840.0
52	44035.0
53	30230.0
54	23050.0
55	15870.0
56	13087.5
57	10305.0
58	9787.5
59	9270.0
60	8277.0
61	7284.0
62	6476.5
63	5669.0
64	4984.5
65	4300.0
66	3653.0
67	3006.0
68	2646.5
69	2287.0
70	1979.5
71	1672.0
72	1416.0
73	1160.0
74	934.0
75	521.0
76	334.0
77	244.5
78	155.0
79	124.5
80	94.0
81	69.5
82	45.0
83	39.0
84	33.0
85	25.0
86	17.0
87	13.0
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1208439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.015047600175347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00435779596916	13.612779561198748
2	7.220584770969052	2.4571718715827924
3	2.6918201773593284	1.3740434534664425
4	1.562036665566785	1.0631251287135208
5	1.0803961286534487	0.9191495778041798
6	0.7678974304046862	0.7839486798232842
7	0.6121149398901851	0.7290615387306979
8	0.46149190444893545	0.6281845377035368
9	0.42841979120308865	0.6560624826159963
>10	3.172672251438817	10.87468600461598
>50	0.5708468525046881	6.989112697162442
>100	1.3795058668309235	53.611447262482
>500	0.04248389749178419	4.913242283025227
>1k	0.0053715272690761625	1.3879849210751427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4835	0.4001029427219744	No Hit
TTTTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1511	0.12503734156213098	No Hit
TTTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1311	0.10848706471737506	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1301	0.10765955087513727	No Hit
AAGTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1271	0.10517700934842389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020108586366378443	0.0
2	0.0	0.0	0.0	0.08531667713471677	0.0
3	0.0	0.0	0.0	0.11320389361813049	0.0
4	0.0	0.0	0.0	0.16575102260023056	0.0
5	0.0	0.0	0.0	0.2701832694906404	0.0
6	0.0	0.0	0.0	0.38438017971945626	0.0
7	0.0	0.0	0.0	0.46473177380074626	0.0
8	0.0	0.0	0.0	0.7095103683346863	0.0
9	0.0	0.0	0.0	0.8084810238663267	0.0
10	0.0	0.0	0.0	0.9603298139169623	0.0
11	0.0	0.0	0.0	1.0962075868124084	0.0
12	0.0	0.0	0.0	1.2124732816468187	0.0
13	0.0	0.0	0.0	1.259310565117478	0.0
14	0.0	0.0	0.0	1.2760263447306814	0.0
15	0.0	0.0	0.0	1.2987829753922209	0.0
16	0.0	0.0	0.0	1.3628325467814262	0.0
17	0.0	0.0	0.0	1.4422738756362548	0.0
18	0.0	0.0	0.0	1.5779861457632534	0.0
19	0.0	0.0	0.0	1.632105551045605	0.0
20	0.0	0.0	0.0	1.6926795642974117	0.0
21	0.0	0.0	0.0	1.7723691473049115	0.0
22	0.0	0.0	0.0	1.847672906948551	0.0
23	0.0	0.0	0.0	1.9462298055590725	0.0
24	0.0	0.0	0.0	2.020706051360474	0.0
25	0.0	0.0	0.0	2.080038793848924	0.0
26	0.0	0.0	0.0	2.142764343090549	0.0
27	0.0	0.0	0.0	2.202179836963223	0.0
28	0.0	0.0	0.0	2.272022005248093	0.0
29	0.0	0.0	0.0	2.3452569802861376	0.0
30	0.0	0.0	0.0	2.455481824072212	0.0
31	0.0	0.0	0.0	2.557266026667461	0.0
32	0.0	0.0	0.0	2.646637521629143	0.0
33	0.0	0.0	0.0	2.731209436305846	0.0
34	0.0	0.0	0.0	2.821904953415108	0.0
35	0.0	0.0	0.0	2.9645683398169043	0.0
36	0.0	0.0	0.0	3.0666007965648245	0.0
37	0.0	0.0	0.0	3.171198546223682	0.0
38	0.0	0.0	0.0	3.2658661297756857	0.0
39	0.0	0.0	0.0	3.372036155734795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	80	0.0	45.000004	28
TCACGAC	40	6.8157533E-9	45.000004	25
CTCACGA	40	6.8157533E-9	45.000004	24
ACAGTCG	55	1.8189894E-12	45.000004	1
ACGTTCG	55	1.8189894E-12	45.000004	2
ACGTTCC	20	7.03343E-4	45.000004	12
TAGGTCG	20	7.03343E-4	45.000004	1
GTTAGAC	20	7.03343E-4	45.000004	39
TACGGAG	20	7.03343E-4	45.000004	4
TCGATAG	20	7.03343E-4	45.000004	1
AACGGTC	20	7.03343E-4	45.000004	42
AACGATT	20	7.03343E-4	45.000004	33
TCCAATC	20	7.03343E-4	45.000004	30
ACGTCAC	40	6.8157533E-9	45.000004	34
TCATACG	40	6.8157533E-9	45.000004	2
CACGTTC	20	7.03343E-4	45.000004	11
AGTAGCG	175	0.0	45.000004	1
CGCGTAG	20	7.03343E-4	45.000004	13
CACAACG	20	7.03343E-4	45.000004	1
TGTAACG	55	1.8189894E-12	45.000004	1
>>END_MODULE