FastQCFastQC Report
Thu 26 May 2016
SRR1545105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545105_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2349195
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166160.7073061197559164No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37150.1581392774971852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34390.14639057208958814No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31980.13613173874454867No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23640.10063021588246185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG1350.045.0000041
TGTTCGA600.045.00000421
ATCGGCA600.045.00000433
GACTCGA302.1664728E-645.00000428
ACGCGAT207.0347916E-445.042
TCGCACC207.0347916E-445.045
TAACGAC207.0347916E-445.032
ACGATTG207.0347916E-445.029
CGACTTA207.0347916E-445.011
ACTCACG453.8562575E-1045.017
TAAGCGA406.8212103E-945.02
TCTCGAT650.044.99999627
CTCGATA650.044.99999628
ACGGGCT2850.042.631585
TATAGCG1750.042.428571
GTACGCG850.042.352941
CAACCGT800.042.187537
CGTTTTT138600.042.1428571
GCGATAT1500.042.09
AGGGATC13000.041.8846176