##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545105_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2349195
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8949005936076	31.0	31.0	33.0	30.0	33.0
2	31.20871915698782	31.0	31.0	33.0	30.0	34.0
3	31.305626821102546	31.0	31.0	34.0	28.0	34.0
4	35.19814106534366	37.0	35.0	37.0	33.0	37.0
5	35.12088055695674	37.0	35.0	37.0	32.0	37.0
6	35.195413748113715	37.0	35.0	37.0	32.0	37.0
7	35.551618320318234	36.0	35.0	37.0	35.0	37.0
8	35.59492592143266	37.0	35.0	37.0	35.0	37.0
9	37.39337049499935	39.0	37.0	39.0	35.0	39.0
10	36.90657182566794	39.0	37.0	39.0	32.0	39.0
11	36.56233688561401	38.0	35.0	39.0	32.0	39.0
12	35.5096196782302	37.0	35.0	39.0	31.0	39.0
13	35.152590142580756	37.0	35.0	39.0	30.0	39.0
14	35.872390755131015	37.0	35.0	40.0	30.0	41.0
15	36.22230593884288	37.0	35.0	40.0	31.0	41.0
16	36.324965786152276	37.0	35.0	40.0	32.0	41.0
17	36.144210250745466	37.0	35.0	40.0	31.0	41.0
18	36.20680956668135	37.0	35.0	40.0	31.0	41.0
19	36.06753292085161	36.0	35.0	40.0	31.0	41.0
20	35.78476541964375	36.0	34.0	40.0	31.0	41.0
21	35.67830256747524	35.0	34.0	40.0	30.0	41.0
22	35.5059081940835	35.0	34.0	40.0	30.0	41.0
23	35.39389620699857	35.0	34.0	40.0	30.0	41.0
24	35.44661979954836	35.0	34.0	40.0	30.0	41.0
25	35.30923146013847	35.0	34.0	40.0	30.0	41.0
26	35.08532880412226	35.0	34.0	39.0	29.0	41.0
27	35.08092176256122	35.0	34.0	40.0	29.0	41.0
28	35.10729845755674	36.0	34.0	40.0	29.0	41.0
29	35.04361068365972	36.0	34.0	40.0	29.0	41.0
30	34.98979011959416	36.0	34.0	40.0	29.0	41.0
31	34.7721900480803	35.0	34.0	40.0	28.0	41.0
32	34.59184444032956	35.0	34.0	40.0	27.0	41.0
33	34.30468139085942	35.0	33.0	40.0	25.0	41.0
34	34.030255044813224	35.0	33.0	40.0	24.0	41.0
35	33.790149817277836	35.0	33.0	40.0	23.0	41.0
36	33.55758674780084	35.0	33.0	40.0	22.0	41.0
37	33.58779965051858	35.0	33.0	40.0	23.0	41.0
38	33.573579034520336	35.0	33.0	40.0	23.0	41.0
39	33.54191457073593	35.0	33.0	40.0	23.0	41.0
40	33.27812718824959	35.0	32.0	40.0	21.0	41.0
41	33.28181866554288	35.0	32.0	39.0	21.0	41.0
42	33.237798054227085	35.0	32.0	39.0	21.0	41.0
43	33.18192487213705	35.0	32.0	39.0	21.0	41.0
44	33.14392036420987	35.0	32.0	39.0	20.0	41.0
45	33.18207428502104	35.0	32.0	39.0	21.0	41.0
46	33.18878254040214	35.0	32.0	39.0	21.0	41.0
47	33.09710092180513	35.0	32.0	39.0	20.0	41.0
48	33.12805322674363	35.0	32.0	39.0	20.0	41.0
49	33.14829462858554	35.0	32.0	39.0	20.0	41.0
50	32.94303878562656	35.0	32.0	39.0	20.0	41.0
51	32.65093872581885	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	5.0
12	4.0
13	4.0
14	27.0
15	58.0
16	224.0
17	620.0
18	1554.0
19	3274.0
20	5658.0
21	9329.0
22	14054.0
23	20616.0
24	28844.0
25	39846.0
26	51018.0
27	56836.0
28	60553.0
29	66280.0
30	76309.0
31	92069.0
32	115744.0
33	151630.0
34	263630.0
35	383584.0
36	146673.0
37	176997.0
38	253073.0
39	330556.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.30958051587884	21.788995804945948	28.726095534853428	16.175328144321778
2	32.61393796598409	25.721406694633693	29.743593018033838	11.921062321348378
3	29.010065149976903	24.979407839706795	33.73811028884363	12.272416721472675
4	25.31216012293573	27.50282543594721	33.82814112919532	13.356873311921744
5	22.424958336791963	32.65386653726063	32.62343057941124	12.297744546536155
6	20.253320818408007	40.20109016067206	30.77986288920247	8.76572613171746
7	83.52388797013445	5.160789121379876	9.825152871515561	1.490170036970111
8	84.84114771230145	3.9822151843503835	9.469073448564295	1.7075636547838728
9	79.88259808147046	6.365925348896111	11.27309567745547	2.4783808921779586
10	52.01466885465022	22.4218508893472	17.116033364620648	8.447446891381942
11	48.15683670363678	18.990803232596697	23.043212674980154	9.809147388786371
12	44.27112266116691	21.25311010793059	24.408318594241855	10.067448636660643
13	18.540947005250736	46.83319179548739	22.987023214335125	11.638837984926752
14	11.95711722526227	46.68148025174581	27.939868763555175	13.421533759436743
15	11.32519863187177	23.31130451069409	53.26437354072352	12.099123316710617
16	11.589501935769487	18.632595420984636	51.3541447176586	18.423757925587275
17	11.659398219390047	19.33670895774936	33.51777949467796	35.48611332818263
18	16.287366523426112	23.460845098001656	43.02422744812585	17.227560930446387
19	31.383388777857945	21.260261493830864	29.406115711977932	17.950234016333255
20	30.942173808474816	21.73186985329017	30.993084865241073	16.33287147299394
21	17.11871513433325	33.84670067831747	31.48955280425848	17.5450313830908
22	18.577980967948594	25.79751787314378	24.741922232935114	30.882578925972513
23	14.755224662065093	32.735596661835224	28.139724458803972	24.36945421729571
24	12.569454642973444	22.20352078052269	51.91301701221056	13.3140075642933
25	12.110701751025351	26.6435097980372	45.299347223197735	15.946441227739715
26	11.36912857383061	39.8485012951245	30.682340120764774	18.100030010280115
27	11.590183020140941	45.34089336985648	28.867718516342833	14.201205093659745
28	11.270413907742865	33.25130523434623	41.62740853781827	13.850872320092627
29	11.568601159120465	25.957189590476737	40.26689993806389	22.207309312338907
30	15.448909094391908	39.88493930899734	30.14888078682272	14.517270809788032
31	29.3536722153759	30.43438284178197	27.503336249225796	12.708608693616325
32	32.51930980612508	24.22570284714551	29.65449866869289	13.600488678036518
33	31.932470484570246	27.820636430777352	23.49770878960665	16.74918429504575
34	18.124634183198925	27.05199014981728	27.540583050789742	27.282792616194058
35	18.86786750354909	25.176539197469772	35.32980446493374	20.625788834047405
36	32.912636030640286	28.836942016307717	24.730130959754298	13.520290993297705
37	16.028341623407165	43.17593899186743	27.917052437111433	12.87866694761397
38	16.002460417291882	41.08718092793489	25.838808613163234	17.07155004161
39	19.246635549624447	39.02839057634637	27.328382701308318	14.396591172720868
40	25.917175883653766	26.19216369862868	27.27410879045801	20.61655162725955
41	14.636120032606915	25.856389103501414	28.88329832133986	30.62419254255181
42	15.958062229827663	23.965188075064013	27.051819878724416	33.02492981638391
43	16.849048291010323	23.8654943501923	29.748020066448294	29.537437292349082
44	13.20707731797488	31.417442996430694	34.45576037749101	20.919719308103417
45	12.323923727063951	47.51734104661384	24.580803211312812	15.577932015009397
46	17.07729669099415	41.58590495893274	25.038704747796587	16.298093602276527
47	16.925287172840058	30.221756814568394	27.532750580518005	25.32020543207354
48	17.14855514335762	27.595069800506135	38.99872083841486	16.25765421772139
49	20.355866584085184	28.972392670680808	36.87505720044526	13.796683544788745
50	17.645023082375026	41.91580520135621	26.382101102718163	14.057070613550598
51	16.824061008132574	36.89191403863877	26.4575311968568	19.826493756371864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4388.0
1	5405.0
2	6422.0
3	19313.5
4	32205.0
5	24793.0
6	17381.0
7	17531.0
8	17681.0
9	18108.5
10	18536.0
11	18332.5
12	18129.0
13	17822.0
14	17515.0
15	16314.0
16	15113.0
17	14778.5
18	14444.0
19	13657.5
20	12871.0
21	13740.0
22	14609.0
23	14543.0
24	14477.0
25	17379.0
26	27222.5
27	34164.0
28	41409.0
29	48654.0
30	59293.5
31	69933.0
32	80887.0
33	91841.0
34	93692.5
35	95544.0
36	103459.0
37	111374.0
38	123472.0
39	135570.0
40	184863.5
41	234157.0
42	271405.0
43	308653.0
44	293153.0
45	277653.0
46	241623.0
47	205593.0
48	173832.5
49	142072.0
50	122499.5
51	102927.0
52	83124.5
53	63322.0
54	51275.0
55	39228.0
56	35976.0
57	32724.0
58	30825.5
59	28927.0
60	27439.5
61	25952.0
62	23372.5
63	20793.0
64	17801.5
65	14810.0
66	12882.0
67	10954.0
68	10266.5
69	9579.0
70	8685.0
71	7791.0
72	6725.0
73	5659.0
74	4637.0
75	2685.0
76	1755.0
77	1349.0
78	943.0
79	707.5
80	472.0
81	369.5
82	267.0
83	193.0
84	119.0
85	87.0
86	55.0
87	41.0
88	27.0
89	18.5
90	10.0
91	7.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2349195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.961822715896787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8179500799161	14.516376915976496
2	6.657909053860325	2.391763641680065
3	2.525245366614979	1.3607402876783412
4	1.4463882016446326	1.0391907382522265
5	0.9887741176562279	0.8880092703704219
6	0.7314920093857348	0.7883357874409007
7	0.6238176824496771	0.7843431833441888
8	0.46298834083584567	0.6652891598096525
9	0.41857038470489566	0.6766458339774648
>10	3.782593859272613	13.840916404667711
>50	0.511597396674456	6.48963257747018
>100	0.8957444233176324	39.3957018981342
>500	0.12646922310904285	13.827533750599988
>1k	0.010222136454302335	2.626024295429741
>5k	0.0	0.0
>10k+	2.3772410358842641E-4	0.7094962551684647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16616	0.7073061197559164	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3715	0.1581392774971852	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3439	0.14639057208958814	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3198	0.13613173874454867	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2364	0.10063021588246185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013579119655882122	0.0
2	0.0	0.0	0.0	0.052145522189515986	0.0
3	0.0	0.0	0.0	0.07947403259414396	0.0
4	0.0	0.0	0.0	0.1318323936497396	0.0
5	0.0	0.0	0.0	0.23595316693590784	0.0
6	0.0	0.0	0.0	0.3926877079169673	0.0
7	0.0	0.0	0.0	0.5113240918697681	0.0
8	0.0	0.0	0.0	0.8560379193723807	0.0
9	0.0	0.0	0.0	0.9953622411081243	0.0
10	0.0	0.0	0.0	1.1944091486658195	0.0
11	0.0	0.0	0.0	1.3308814295960958	0.0
12	0.0	0.0	0.0	1.456115818397366	0.0
13	0.0	0.0	0.0	1.5077505273082907	0.0
14	0.0	0.0	0.0	1.5272891352144031	0.0
15	0.0	0.0	0.0	1.5538514257011444	0.0
16	0.0	0.0	0.0	1.6123395460998342	0.0
17	0.0	0.0	0.0	1.6796391955542218	0.0
18	0.0	0.0	0.0	1.7938059633193497	0.0
19	0.0	0.0	0.0	1.838970370701453	0.0
20	0.0	0.0	0.0	1.887369928847967	0.0
21	0.0	0.0	0.0	1.956329721457776	0.0
22	0.0	0.0	0.0	2.0255023529336644	0.0
23	0.0	0.0	0.0	2.1129365591191878	0.0
24	0.0	0.0	0.0	2.1806193185325187	0.0
25	0.0	0.0	0.0	2.234339848331024	0.0
26	0.0	0.0	0.0	2.2924022909975545	0.0
27	0.0	0.0	0.0	2.3483363450032884	0.0
28	0.0	0.0	0.0	2.40963393843423	0.0
29	0.0	0.0	0.0	2.4800836031065963	0.0
30	0.0	0.0	0.0	2.5842043763927642	0.0
31	0.0	0.0	0.0	2.683983236810908	0.0
32	0.0	0.0	0.0	2.7734606961107953	0.0
33	0.0	0.0	0.0	2.858809081408738	0.0
34	0.0	0.0	0.0	2.952458182483787	0.0
35	0.0	0.0	0.0	3.0713499730758835	0.0
36	0.0	0.0	0.0	3.172746408876232	0.0
37	0.0	0.0	0.0	3.2748239290480354	0.0
38	0.0	0.0	0.0	3.3780507790966694	0.0
39	0.0	0.0	0.0	3.4803837059077685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	135	0.0	45.000004	1
TGTTCGA	60	0.0	45.000004	21
ATCGGCA	60	0.0	45.000004	33
GACTCGA	30	2.1664728E-6	45.000004	28
ACGCGAT	20	7.0347916E-4	45.0	42
TCGCACC	20	7.0347916E-4	45.0	45
TAACGAC	20	7.0347916E-4	45.0	32
ACGATTG	20	7.0347916E-4	45.0	29
CGACTTA	20	7.0347916E-4	45.0	11
ACTCACG	45	3.8562575E-10	45.0	17
TAAGCGA	40	6.8212103E-9	45.0	2
TCTCGAT	65	0.0	44.999996	27
CTCGATA	65	0.0	44.999996	28
ACGGGCT	285	0.0	42.63158	5
TATAGCG	175	0.0	42.42857	1
GTACGCG	85	0.0	42.35294	1
CAACCGT	80	0.0	42.1875	37
CGTTTTT	13860	0.0	42.142857	1
GCGATAT	150	0.0	42.0	9
AGGGATC	1300	0.0	41.884617	6
>>END_MODULE