FastQCFastQC Report
Thu 26 May 2016
SRR1545104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545104_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4275355
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315870.7388158410237279No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG66320.1551216214793859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT253.8925922E-545.00000439
CGATCCA207.0354406E-445.00000430
GTACGAT207.0354406E-445.00000427
CGTATCA453.8562575E-1045.037
CGTTCGA950.045.014
GTATGCG950.045.01
TGTTACG1700.043.6764681
CGTTAGG5050.043.2178232
CGTTTTT209250.042.7311821
ACGTTAG1900.042.631581
TAATGCG1750.042.4285741
GCGATCG850.042.352949
TAATACG1400.041.7857131
TCTAGCG2100.041.7857131
ACTACGG2000.041.6250042
TACGGGC3600.041.254
ACGGGCC2350.041.170215
CTATGCG1050.040.7142831
GACCGAT7550.040.52989
CGTAGAT501.0822987E-940.50000427
Produced by FastQC (version 0.11.5)