##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545104_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4275355
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.834781439202125	31.0	31.0	33.0	28.0	33.0
2	31.1581143554161	31.0	31.0	33.0	30.0	34.0
3	31.253425505016544	31.0	31.0	34.0	28.0	34.0
4	35.12950877763367	35.0	35.0	37.0	32.0	37.0
5	35.02233218060255	37.0	35.0	37.0	32.0	37.0
6	35.0942010663442	37.0	35.0	37.0	32.0	37.0
7	35.51414981913783	36.0	35.0	37.0	35.0	37.0
8	35.572820034827515	37.0	35.0	37.0	35.0	37.0
9	37.3726658955806	39.0	37.0	39.0	35.0	39.0
10	36.89353679402061	39.0	37.0	39.0	32.0	39.0
11	36.459569556212294	38.0	35.0	39.0	32.0	39.0
12	35.25230396072373	35.0	35.0	39.0	31.0	39.0
13	34.813992054460975	35.0	34.0	39.0	30.0	39.0
14	35.45934314226538	36.0	33.0	40.0	27.0	41.0
15	35.893877818333216	36.0	34.0	40.0	30.0	41.0
16	36.04556019324711	36.0	34.0	40.0	31.0	41.0
17	35.88081059935374	36.0	34.0	40.0	31.0	41.0
18	35.93414862625443	36.0	35.0	40.0	31.0	41.0
19	35.80644578052583	36.0	35.0	40.0	30.0	41.0
20	35.528396823187784	35.0	34.0	40.0	30.0	41.0
21	35.387531561706574	35.0	34.0	40.0	30.0	41.0
22	35.219619189517594	35.0	34.0	40.0	29.0	41.0
23	35.152221043632636	35.0	34.0	40.0	29.0	41.0
24	35.238634920375034	35.0	34.0	40.0	30.0	41.0
25	35.12652516574647	35.0	34.0	39.0	29.0	41.0
26	34.8388040291391	35.0	34.0	39.0	29.0	41.0
27	34.8024678652416	35.0	34.0	39.0	29.0	41.0
28	34.872111906496656	36.0	34.0	39.0	29.0	41.0
29	34.84077649692248	36.0	34.0	39.0	29.0	41.0
30	34.79961547052818	36.0	34.0	39.0	29.0	41.0
31	34.56193392127671	35.0	34.0	39.0	27.0	41.0
32	34.38981534866695	35.0	34.0	39.0	26.0	41.0
33	34.11316908186571	35.0	33.0	40.0	25.0	41.0
34	33.85804734343698	35.0	33.0	39.0	23.0	41.0
35	33.61374295233963	35.0	33.0	39.0	23.0	41.0
36	33.384617651633604	35.0	33.0	39.0	22.0	41.0
37	33.41249627223938	35.0	33.0	39.0	23.0	41.0
38	33.42047923505768	35.0	32.0	39.0	23.0	41.0
39	33.407529199329645	35.0	33.0	39.0	23.0	41.0
40	33.13014311092295	35.0	32.0	39.0	21.0	41.0
41	33.14386103610109	35.0	32.0	39.0	22.0	41.0
42	33.12000851391288	35.0	32.0	39.0	22.0	41.0
43	33.066717032854584	35.0	32.0	39.0	21.0	41.0
44	33.02896414449794	35.0	32.0	39.0	21.0	41.0
45	33.10264691470065	35.0	32.0	39.0	22.0	41.0
46	33.11628975839433	35.0	32.0	39.0	23.0	41.0
47	33.018258132950365	35.0	32.0	39.0	22.0	41.0
48	33.0353558944228	35.0	32.0	39.0	22.0	41.0
49	33.08850376167593	35.0	32.0	39.0	22.0	41.0
50	32.93630493842032	35.0	32.0	39.0	21.0	41.0
51	32.65782513966676	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	5.0
12	18.0
13	27.0
14	58.0
15	144.0
16	431.0
17	1413.0
18	3252.0
19	6500.0
20	11237.0
21	18021.0
22	26662.0
23	38462.0
24	53862.0
25	73910.0
26	93508.0
27	106266.0
28	113133.0
29	125428.0
30	147106.0
31	177296.0
32	220733.0
33	289168.0
34	504038.0
35	714783.0
36	261459.0
37	310449.0
38	432580.0
39	545206.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.169879460302123	24.344855573396828	28.764699071772988	15.720565894528058
2	33.42492026977877	26.00467095714859	28.636358852071936	11.934049921000712
3	28.459578210464393	24.550546095002638	33.94190657851804	13.047969116014926
4	25.86360664786901	27.85237717101855	33.1344414674337	13.149574713678746
5	22.795042750835893	32.56248896290484	32.03303585316307	12.609432433096199
6	21.22696243937638	39.73896904467582	29.929982422512282	9.104086093435516
7	83.66636220851835	5.383576334596777	9.41809510555264	1.5319663513322284
8	85.29483516573477	3.9559989755236695	9.239747342618331	1.5094185161232225
9	80.31814901920426	6.183229228917832	10.961358764359918	2.5372629875179955
10	56.35482901419883	20.585846087634827	15.85007560775655	7.209249290409803
11	51.79361713822595	18.44139258611273	21.894953752378456	7.870036523282862
12	46.668148025134755	21.667557430903397	22.585609849942283	9.078684694019561
13	18.56168669034501	47.125583723456884	23.000709882571154	11.31201970362695
14	11.274174893079055	47.82234457723394	28.951163119787715	11.952317409899296
15	10.790332030907376	24.019806542380692	54.395436168458524	10.794425258253408
16	12.16146963234632	18.970681966760655	52.99414902388223	15.873699377010798
17	12.474238981324358	19.856900771982676	31.30313155281842	36.365728693874544
18	16.82938609776264	23.730941641103488	43.36128812695086	16.07838413418301
19	32.448065716180295	20.304957132214753	29.241735481614977	18.005241669989978
20	31.674071509851228	22.736451125111248	30.379956752129356	15.209520612908168
21	17.957923961869835	34.06921764391495	32.10608241888686	15.866775975328364
22	19.548598888279454	25.157489845872448	25.055790688726432	30.238120577121663
23	14.62276232032194	33.4706942464427	28.330676633870173	23.5758667993652
24	11.985180178020304	22.292721890930693	52.413238198933186	13.308859732115813
25	12.119297695746903	26.193240093512703	46.478175496537716	15.20928671420268
26	11.529662449083174	40.8100847765858	30.23236666896667	17.427886105364347
27	11.924974651227792	46.67086125011841	28.94533904202107	12.458825056632724
28	10.63612261437939	33.77190899936964	42.56507354360048	13.026894842650494
29	11.142653650983368	26.536229155239738	42.01089734068867	20.310219853088224
30	15.353344926912502	40.91363173350517	29.713134932654718	14.019888406927612
31	30.9427872071442	30.066649436128696	26.72168743882087	12.268875917906232
32	33.93060927104299	23.947204384197335	29.71800002572886	12.404186319030817
33	33.368129664086375	27.723475594424325	23.13793825308074	15.770456488408566
34	18.973418581614858	27.175029909796965	26.901064356059322	26.950487152528858
35	19.408633902915664	25.18055225823353	35.47803632680795	19.93277751204286
36	34.931882849494365	27.698261313972754	24.19602582709506	13.173830009437811
37	16.682404151234227	43.07967408554377	28.083960279321836	12.153961483900169
38	16.44817798755893	42.41844244513029	24.04766387820427	17.085715689106518
39	19.91425273456824	39.48182080786274	26.404520794179664	14.199405663389356
40	27.21680421859705	25.653284931894543	25.934197277185167	21.195713572323232
41	14.343066248299849	25.173628856551094	28.558447193274013	31.924857701875048
42	16.120205222724195	23.543846066583946	26.60819978691828	33.72774892377358
43	17.016247773576698	23.196155640876608	30.081010816645637	29.70658576890106
44	13.048857931095782	30.810587658802604	35.09032583259168	21.050228577509937
45	12.17276693982137	49.22849681488437	23.590134620399944	15.008601624894307
46	17.893812326695677	41.407649189365564	24.813799088028947	15.884739395909813
47	17.528228650018537	29.353024485685985	26.86466971748545	26.25407714681003
48	17.154692417354816	28.205002859411678	39.385337591849094	15.254967131384412
49	20.733389391056413	28.313438299275735	37.37836974941262	13.57480256025523
50	18.03515731442184	42.33854264733572	25.719010468136567	13.907289570105874
51	16.132601854115038	39.21260339784649	25.49856093821449	19.15623380982398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5811.0
1	9120.5
2	12430.0
3	35480.5
4	58531.0
5	44792.5
6	31054.0
7	31502.0
8	31950.0
9	33897.5
10	35845.0
11	35167.0
12	34489.0
13	33630.5
14	32772.0
15	30936.5
16	29101.0
17	28851.5
18	28602.0
19	27322.5
20	26043.0
21	26705.5
22	27368.0
23	29143.0
24	30918.0
25	37500.0
26	51234.5
27	58387.0
28	70843.5
29	83300.0
30	100558.5
31	117817.0
32	124368.5
33	130920.0
34	153978.0
35	177036.0
36	185144.0
37	193252.0
38	220359.0
39	247466.0
40	342878.0
41	438290.0
42	515410.5
43	592531.0
44	545957.5
45	499384.0
46	436925.5
47	374467.0
48	320404.5
49	266342.0
50	220802.5
51	175263.0
52	141816.0
53	108369.0
54	91067.0
55	73765.0
56	66977.0
57	60189.0
58	59488.0
59	58787.0
60	54658.0
61	50529.0
62	45127.0
63	39725.0
64	34445.5
65	29166.0
66	25116.5
67	21067.0
68	18167.5
69	15268.0
70	13890.0
71	12512.0
72	10883.5
73	9255.0
74	7580.5
75	4598.0
76	3290.0
77	2687.0
78	2084.0
79	1526.0
80	968.0
81	732.5
82	497.0
83	395.5
84	294.0
85	209.5
86	125.0
87	103.5
88	82.0
89	45.5
90	9.0
91	9.0
92	9.0
93	8.0
94	7.0
95	3.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4275355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.299787769344054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01630379276241	13.205485576787309
2	7.619324703138268	2.4838675121374827
3	2.5554090060572814	1.2495787338781235
4	1.2880240172117305	0.8397807248947665
5	0.7711435058201637	0.6284737742288299
6	0.6127855943255197	0.5992965081370399
7	0.45104248040347195	0.5146327693874581
8	0.3878478913204988	0.5057470652249398
9	0.3065220408470109	0.44966197911892375
>10	3.252319656451128	11.574087241332084
>50	0.6062156949674647	6.960378500111723
>100	0.945087254650422	38.07840532768833
>500	0.16599582125161821	17.16995502970952
>1k	0.02168934946667485	4.839873817173111
>5k	1.445956631111657E-4	0.1563081901499972
>10k+	1.445956631111657E-4	0.7444672500404043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31587	0.7388158410237279	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6632	0.1551216214793859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3389870548761448E-5	0.0	0.0	0.022150207409677092	0.0
2	2.3389870548761448E-5	0.0	0.0	0.07585335018963338	0.0
3	2.3389870548761448E-5	0.0	0.0	0.10953476377984986	0.0
4	2.3389870548761448E-5	0.0	0.0	0.1594955272720043	0.0
5	2.3389870548761448E-5	0.0	0.0	0.29045541247451967	0.0
6	2.3389870548761448E-5	0.0	0.0	0.42230411275788793	0.0
7	2.3389870548761448E-5	0.0	0.0	0.5160974936584214	0.0
8	2.3389870548761448E-5	0.0	0.0	0.6878259232274279	0.0
9	2.3389870548761448E-5	0.0	0.0	0.7550484111845683	0.0
10	2.3389870548761448E-5	0.0	0.0	0.8510170500461365	0.0
11	2.3389870548761448E-5	0.0	0.0	0.97294844521683	0.0
12	2.3389870548761448E-5	0.0	0.0	1.0599821535287712	0.0
13	2.3389870548761448E-5	0.0	0.0	1.0999788321671533	0.0
14	2.3389870548761448E-5	0.0	0.0	1.1177083540431145	0.0
15	2.3389870548761448E-5	0.0	0.0	1.1427822952713869	0.0
16	2.3389870548761448E-5	0.0	0.0	1.1972573037794523	0.0
17	2.3389870548761448E-5	0.0	0.0	1.2595679189213527	0.0
18	2.3389870548761448E-5	0.0	0.0	1.3503907862621933	0.0
19	2.3389870548761448E-5	0.0	0.0	1.3961413730555707	0.0
20	2.3389870548761448E-5	0.0	0.0	1.4489557007546743	0.0
21	2.3389870548761448E-5	0.0	0.0	1.5223765044072364	0.0
22	2.3389870548761448E-5	0.0	0.0	1.6048257980916205	0.0
23	2.3389870548761448E-5	0.0	0.0	1.699507994073007	0.0
24	2.3389870548761448E-5	0.0	0.0	1.7719698130330697	0.0
25	2.3389870548761448E-5	0.0	0.0	1.8341634788222265	0.0
26	2.3389870548761448E-5	0.0	0.0	1.9037717335753406	0.0
27	2.3389870548761448E-5	0.0	0.0	1.9721871049304678	0.0
28	2.3389870548761448E-5	0.0	0.0	2.041959088777423	0.0
29	2.3389870548761448E-5	0.0	0.0	2.1252504178015625	0.0
30	2.3389870548761448E-5	0.0	0.0	2.2321421262094026	0.0
31	2.3389870548761448E-5	0.0	0.0	2.330145683808713	0.0
32	2.3389870548761448E-5	0.0	0.0	2.4152848126062048	0.0
33	2.3389870548761448E-5	0.0	0.0	2.501523265319488	0.0
34	2.3389870548761448E-5	0.0	0.0	2.599292924213311	0.0
35	2.3389870548761448E-5	0.0	0.0	2.7321006091891786	0.0
36	2.3389870548761448E-5	0.0	0.0	2.836676720412691	0.0
37	2.3389870548761448E-5	0.0	0.0	2.946094534839797	0.0
38	2.3389870548761448E-5	0.0	0.0	3.0615703257390323	0.0
39	2.3389870548761448E-5	0.0	0.0	3.240035038026082	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	25	3.8925922E-5	45.000004	39
CGATCCA	20	7.0354406E-4	45.000004	30
GTACGAT	20	7.0354406E-4	45.000004	27
CGTATCA	45	3.8562575E-10	45.0	37
CGTTCGA	95	0.0	45.0	14
GTATGCG	95	0.0	45.0	1
TGTTACG	170	0.0	43.676468	1
CGTTAGG	505	0.0	43.217823	2
CGTTTTT	20925	0.0	42.731182	1
ACGTTAG	190	0.0	42.63158	1
TAATGCG	175	0.0	42.428574	1
GCGATCG	85	0.0	42.35294	9
TAATACG	140	0.0	41.785713	1
TCTAGCG	210	0.0	41.785713	1
ACTACGG	200	0.0	41.625004	2
TACGGGC	360	0.0	41.25	4
ACGGGCC	235	0.0	41.17021	5
CTATGCG	105	0.0	40.714283	1
GACCGAT	755	0.0	40.5298	9
CGTAGAT	50	1.0822987E-9	40.500004	27
>>END_MODULE