FastQCFastQC Report
Thu 26 May 2016
SRR1545102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545102_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences765726
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37560.4905148839140894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT750.045.00000411
ACAACGG453.8380676E-1045.02
CTGCTCG253.889581E-545.010
CTTCGAT207.03181E-445.011
CGGAATC253.889581E-545.015
CGGCGCT207.03181E-445.010
CGAACGG406.8102963E-945.02
GCGACCT453.8380676E-1045.011
GCGACCC406.8102963E-945.031
GTCGAAA253.889581E-545.019
CTCCGCC253.889581E-545.045
GAAGACG406.8102963E-945.01
CACGGGC207.03181E-445.04
CACGGGA900.045.04
TCCGATA253.889581E-545.010
ACGTTCA207.03181E-445.021
CCGATCA351.2112105E-745.01
ACGTTAG253.889581E-545.01
GCGCGAC406.8102963E-945.09
GCAACGA207.03181E-445.09