FastQCFastQC Report
Thu 26 May 2016
SRR1545101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545101_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2007277
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60990.30384446192528486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24300.12105952491858374No Hit
AGTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA20590.10257677440632258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG850.045.0000041
AGAGTAC750.045.00000410
GTTACGG850.045.0000042
CGTAGTT850.045.00000414
GTATGCG1500.045.0000041
TGTTCGG1250.045.0000042
ACGTGAG850.045.0000041
GCGATGA1050.045.0000049
TCGTAGT850.045.00000413
TGCTACG1500.045.0000041
CTTGTAG800.045.01
CGGAACT406.8193913E-945.023
AATCCCG453.8562575E-1045.02
ACCGGTG406.8193913E-945.019
AAATCGA406.8193913E-945.026
TCGCAAG2250.045.01
TACCCAA551.8189894E-1245.039
ACACGAT207.0345466E-445.039
GCGACAA351.2125383E-745.01
GTCGATA253.8918504E-545.09
Produced by FastQC (version 0.11.5)