##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545101_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2007277
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.132298133242198	31.0	31.0	33.0	30.0	33.0
2	31.472739935743796	31.0	31.0	34.0	30.0	34.0
3	31.614035332442906	31.0	31.0	34.0	30.0	34.0
4	35.42728980604072	37.0	35.0	37.0	33.0	37.0
5	35.36312875602122	37.0	35.0	37.0	33.0	37.0
6	35.420643488666485	37.0	35.0	37.0	33.0	37.0
7	35.74675543036661	37.0	35.0	37.0	35.0	37.0
8	35.83898584998483	37.0	35.0	37.0	35.0	37.0
9	37.768841569947746	39.0	38.0	39.0	35.0	39.0
10	37.38232192168794	39.0	37.0	39.0	35.0	39.0
11	36.802486154128204	39.0	35.0	39.0	33.0	39.0
12	34.69149100996026	35.0	34.0	38.0	31.0	39.0
13	34.0254499005369	35.0	33.0	38.0	26.0	39.0
14	34.49174030290787	35.0	33.0	39.0	25.0	41.0
15	35.148020427673906	35.0	33.0	39.0	30.0	41.0
16	35.49748490118703	35.0	34.0	39.0	32.0	41.0
17	35.41387810451672	35.0	34.0	38.0	32.0	41.0
18	35.58364590437692	36.0	35.0	38.0	31.0	41.0
19	35.45032399614004	36.0	35.0	38.0	31.0	41.0
20	35.07032512204344	35.0	34.0	38.0	31.0	41.0
21	34.865878501073844	35.0	34.0	38.0	31.0	41.0
22	34.70620796232907	35.0	34.0	38.0	30.0	41.0
23	34.75824711786166	35.0	34.0	38.0	30.0	41.0
24	34.88033290871165	35.0	34.0	38.0	31.0	41.0
25	34.78321726398499	35.0	34.0	37.0	31.0	41.0
26	34.592679535510044	35.0	34.0	37.0	31.0	40.0
27	34.56489562726021	35.0	34.0	37.0	30.0	40.0
28	34.78589950465232	35.0	34.0	37.0	31.0	40.0
29	34.85286983311222	35.0	34.0	37.0	31.0	40.0
30	34.81529006709089	35.0	34.0	37.0	31.0	40.0
31	34.42867426867343	35.0	34.0	37.0	30.0	40.0
32	34.21389773309812	35.0	34.0	37.0	29.0	40.0
33	33.956056388829246	35.0	33.0	37.0	29.0	40.0
34	33.831522007176886	35.0	34.0	37.0	27.0	40.0
35	33.57433876839121	35.0	33.0	37.0	27.0	40.0
36	33.25090209273558	35.0	33.0	37.0	23.0	40.0
37	33.268236521416824	35.0	33.0	37.0	24.0	40.0
38	33.395628505682076	35.0	33.0	36.0	27.0	40.0
39	33.42427627078874	35.0	33.0	36.0	25.0	40.0
40	33.00336276458107	35.0	32.0	36.0	23.0	40.0
41	33.257193202532584	35.0	33.0	36.0	23.0	40.0
42	33.23053121218447	35.0	33.0	36.0	23.0	40.0
43	33.19555547141725	35.0	33.0	36.0	24.0	40.0
44	33.176924261076074	35.0	33.0	36.0	23.0	40.0
45	33.214343610772204	35.0	33.0	36.0	24.0	40.0
46	33.294631483347835	35.0	33.0	36.0	25.0	40.0
47	33.284643823448384	35.0	33.0	36.0	26.0	40.0
48	33.35103924371175	35.0	33.0	36.0	25.0	40.0
49	33.47537534680067	35.0	33.0	36.0	24.0	40.0
50	33.161708623174576	35.0	33.0	36.0	24.0	40.0
51	32.85121684750037	35.0	33.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	2.0
13	5.0
14	12.0
15	20.0
16	97.0
17	296.0
18	729.0
19	1630.0
20	3009.0
21	5193.0
22	8182.0
23	12734.0
24	18827.0
25	27556.0
26	35785.0
27	41303.0
28	45515.0
29	51637.0
30	63387.0
31	80977.0
32	109145.0
33	154420.0
34	328180.0
35	524078.0
36	77110.0
37	90652.0
38	129742.0
39	196892.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.428800310071804	26.013848611825875	33.3715775152109	13.18577356289142
2	29.454629331178506	26.235193249362194	32.096367367333954	12.213810052125341
3	27.401051274936144	24.09204110842699	36.244026110995144	12.262881505641722
4	22.94396837108182	29.452487125593528	34.51830514672364	13.085239356601008
5	22.388738574695967	32.733449344559816	33.36674509796107	11.511066982783143
6	20.551324007598353	40.0372245584441	29.48955226408712	9.921899169870427
7	88.60849798009941	3.9860965875661405	6.193763989723392	1.2116414426110598
8	92.36373455183315	1.6325599306921765	5.331700607340193	0.6720049101344757
9	90.23184144490271	2.6918058643625167	6.12122791224131	0.9551247784934516
10	77.23702309148165	10.281690070677838	8.845963960131064	3.6353228777094544
11	74.70234551584062	9.286112479742457	12.109389984541247	3.902152019875682
12	62.10209153993196	19.673966273713095	13.288001606156003	4.935940580198946
13	15.095325657594842	65.66149066621098	14.19415456860214	5.049029107592026
14	6.251952271659567	62.73454037484613	26.102227046889894	4.91128030660442
15	4.7574400543622035	17.947747122096253	71.84987423260468	5.444938590936877
16	5.094413974752862	10.74017188459789	68.23432939250537	15.931084748143878
17	6.5567432895410045	10.32000067753479	30.88069060722561	52.242565425698594
18	15.624051887208394	17.98964467783968	50.802803997654536	15.583499437297393
19	40.914482654860294	13.143029088660906	28.534377666859136	17.408110589619668
20	39.88293593759107	17.838893187138595	26.18492614621699	16.09324472905334
21	15.036987919455063	36.36194705563806	31.42466136960669	17.17640365530019
22	17.16031220404558	22.577800672254003	18.127592753765427	42.13429436993499
23	9.061728899399535	36.62653435475024	24.86373330636479	29.448003439485433
24	7.813819418047434	15.95828577719966	65.3873381700682	10.840556634684699
25	6.0876999038996615	21.564138880682638	56.76650507129808	15.581656144119622
26	5.224092140745896	49.37963220821043	29.41387760632937	15.982398044714309
27	5.5848295975094615	59.786865489914945	26.345541746355885	8.282763166219711
28	4.764464495931553	34.86564136389746	51.45801999425091	8.91187414592007
29	5.863814510902083	23.884645716560296	47.92178657953038	22.329753193007242
30	13.232304260946545	47.95865244308583	28.89342128664853	9.915622009319094
31	41.27850814810313	27.007184359707203	24.244287161164106	7.470020331025563
32	46.14400503767044	16.82368701479666	30.18721382250681	6.845094125026092
33	44.60296212231795	23.415502693449884	17.256213268024293	14.725321916207879
34	20.286985802158846	23.398016317628308	22.173322366569238	34.14167551364361
35	18.203317230257706	21.246345173087718	39.76675864865686	20.78357894799771
36	47.84725775266692	21.54924307905685	21.416725245195355	9.186773923080871
37	14.854800807262775	50.77445713770447	25.835198629785527	8.535543425247239
38	15.91907843312109	49.79945468413179	17.677879037123425	16.6035878456237
39	20.99535838850343	44.49445691850203	23.39403081886556	11.116153874128981
40	33.51694858258227	19.988372307359672	21.488264947986753	25.006414162071305
41	11.220324848040406	19.50224109577303	25.352953279492567	43.924480776694
42	15.818295133158006	15.178074575656474	24.528851772824577	44.47477851836094
43	15.215139714150064	15.665301799402872	30.099632487195336	39.01992599925172
44	8.682907241999983	30.862506769120557	39.90958896056698	20.544997028312483
45	9.147865491409506	61.14686712396944	18.550005803882573	11.155261580738483
46	18.079517674939733	45.98916841073753	21.570615316172105	14.360698598150629
47	16.404811094831455	27.82889456711754	23.55609116230595	32.21020317574505
48	18.53829840126699	23.933717170076676	45.391443233793844	12.136541194862493
49	23.617567480721398	23.978703487361237	42.397486744480204	10.00624228743716
50	17.958757062428354	51.758277507289726	19.826610876326487	10.456354553955432
51	15.651352553733242	45.46223565556722	21.65869483882892	17.22771695187062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3125.0
1	3261.0
2	3397.0
3	7493.0
4	11589.0
5	9677.5
6	7766.0
7	8795.5
8	9825.0
9	10930.5
10	12036.0
11	12465.5
12	12895.0
13	12718.5
14	12542.0
15	11600.0
16	10658.0
17	9436.5
18	8215.0
19	8195.0
20	8175.0
21	7060.5
22	5946.0
23	7526.5
24	9107.0
25	9344.5
26	10294.0
27	11006.0
28	14412.5
29	17819.0
30	21954.5
31	26090.0
32	34214.5
33	42339.0
34	46619.5
35	50900.0
36	54883.5
37	58867.0
38	87613.5
39	116360.0
40	192077.5
41	267795.0
42	326185.0
43	384575.0
44	370304.5
45	356034.0
46	294543.5
47	233053.0
48	184261.5
49	135470.0
50	110203.0
51	84936.0
52	63539.0
53	42142.0
54	30273.5
55	18405.0
56	13938.5
57	9472.0
58	8306.5
59	7141.0
60	6620.0
61	6099.0
62	5459.5
63	4820.0
64	3840.5
65	2861.0
66	2540.5
67	2220.0
68	1956.5
69	1693.0
70	1296.0
71	899.0
72	744.5
73	590.0
74	487.0
75	298.0
76	212.0
77	158.5
78	105.0
79	90.5
80	76.0
81	55.5
82	35.0
83	24.5
84	14.0
85	8.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2007277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.42585010454599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.66676623170667	10.023531456136393
2	7.92998047991741	1.9707349755085886
3	2.5897310059471574	0.9653882787298343
4	1.3168755562855172	0.6545319307497781
5	0.8065313947836538	0.5010919108096045
6	0.5920344334904779	0.4413918676369488
7	0.41523480477491986	0.36117518096265006
8	0.35706896771889773	0.35495083758879953
9	0.3069119311552136	0.343227748664828
>10	3.005115935482865	8.00262784498531
>50	0.45821839430117234	4.054701372656557
>100	1.210661230925436	42.2439519172536
>500	0.30467503753037206	23.413611683390336
>1k	0.039792650020457565	6.364467205084509
>5k	4.019459598026016E-4	0.30461578984226845
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6099	0.30384446192528486	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2430	0.12105952491858374	No Hit
AGTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2059	0.10257677440632258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017934744432382774	0.0
2	0.0	0.0	0.0	0.07288480862382223	0.0
3	0.0	0.0	0.0	0.10093275616668751	0.0
4	0.0	0.0	0.0	0.1462678045929884	0.0
5	0.0	0.0	0.0	0.23748590752546858	0.0
6	0.0	0.0	0.0	0.3193879071000166	0.0
7	0.0	0.0	0.0	0.3796685758866365	0.0
8	0.0	0.0	0.0	0.5289753232862231	0.0
9	0.0	0.0	0.0	0.5797904325113076	0.0
10	0.0	0.0	0.0	0.660347326253427	0.0
11	0.0	0.0	0.0	0.7650663062447286	0.0
12	0.0	0.0	0.0	0.8608179140198389	0.0
13	0.0	0.0	0.0	0.9182090962034637	0.0
14	0.0	0.0	0.0	0.9466555936226042	0.0
15	0.0	0.0	0.0	0.9725115168459559	0.0
16	0.0	0.0	0.0	1.0250702817797444	0.0
17	0.0	0.0	0.0	1.085948775380777	0.0
18	0.0	0.0	0.0	1.1933081483024017	0.0
19	0.0	0.0	0.0	1.2354547977185013	0.0
20	0.0	0.0	0.0	1.278896734232495	0.0
21	0.0	0.0	0.0	1.3497887934749415	0.0
22	0.0	0.0	0.0	1.4091727250399422	0.0
23	0.0	0.0	0.0	1.4982486223874434	0.0
24	0.0	0.0	0.0	1.5533979615170204	0.0
25	0.0	0.0	0.0	1.596192254482067	0.0
26	0.0	0.0	0.0	1.6541812614801046	0.0
27	0.0	0.0	0.0	1.6996159473754744	0.0
28	0.0	0.0	0.0	1.7480397573429078	0.0
29	0.0	0.0	0.0	1.8042851086322416	0.0
30	0.0	0.0	0.0	1.8971970485388912	0.0
31	0.0	0.0	0.0	1.9988770857235947	0.0
32	0.0	0.0	0.0	2.070765519656729	0.0
33	0.0	0.0	0.0	2.1405117480048843	0.0
34	0.0	0.0	0.0	2.222911934924776	0.0
35	0.0	0.0	0.0	2.3681833648270767	0.0
36	0.0	0.0	0.0	2.4551668753241334	0.0
37	0.0	0.0	0.0	2.5519148577899315	0.0
38	0.0	0.0	0.0	2.626144772246182	0.0
39	0.0	0.0	0.0	2.7104380710783813	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCG	85	0.0	45.000004	1
AGAGTAC	75	0.0	45.000004	10
GTTACGG	85	0.0	45.000004	2
CGTAGTT	85	0.0	45.000004	14
GTATGCG	150	0.0	45.000004	1
TGTTCGG	125	0.0	45.000004	2
ACGTGAG	85	0.0	45.000004	1
GCGATGA	105	0.0	45.000004	9
TCGTAGT	85	0.0	45.000004	13
TGCTACG	150	0.0	45.000004	1
CTTGTAG	80	0.0	45.0	1
CGGAACT	40	6.8193913E-9	45.0	23
AATCCCG	45	3.8562575E-10	45.0	2
ACCGGTG	40	6.8193913E-9	45.0	19
AAATCGA	40	6.8193913E-9	45.0	26
TCGCAAG	225	0.0	45.0	1
TACCCAA	55	1.8189894E-12	45.0	39
ACACGAT	20	7.0345466E-4	45.0	39
GCGACAA	35	1.2125383E-7	45.0	1
GTCGATA	25	3.8918504E-5	45.0	9
>>END_MODULE