FastQCFastQC Report
Thu 26 May 2016
SRR1545100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545100_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4628446
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231010.4991092042555968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC351.2129931E-745.00000420
GTCGGTT1100.045.00000445
ATCGTCC253.892643E-545.021
GACCGTA302.166933E-644.9999969
CTTGACG2200.043.9772761
CGGGTAT3100.042.822586
TCTAGCG1900.042.631581
CTACGCG850.042.3529431
ACTACGG3400.042.3529432
CGTTTTT205250.041.7880631
TGATCCG700.041.7857171
TGATCGA650.041.5384629
AACCGTA650.041.5384634
GGTACGC650.041.538468
CTAAGCG650.041.538461
TACGACC650.041.5384639
ATCGAAT650.041.5384631
GTACGCG1300.041.538461
CCCGCTA603.6379788E-1241.24999633
TAAGTCG556.184564E-1140.9090921
Produced by FastQC (version 0.11.5)