##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545100_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4628446
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94998602122613	31.0	31.0	33.0	30.0	33.0
2	31.274396633340867	31.0	31.0	33.0	30.0	34.0
3	31.392097693264652	31.0	31.0	34.0	30.0	34.0
4	35.26113581102599	37.0	35.0	37.0	33.0	37.0
5	35.166513987632136	37.0	35.0	37.0	32.0	37.0
6	35.235849570244525	37.0	35.0	37.0	32.0	37.0
7	35.62347556825768	36.0	35.0	37.0	35.0	37.0
8	35.693875006859756	37.0	35.0	37.0	35.0	37.0
9	37.54092777575886	39.0	37.0	39.0	35.0	39.0
10	37.01431733242648	39.0	37.0	39.0	33.0	39.0
11	36.60175531917192	39.0	35.0	39.0	32.0	39.0
12	35.535428953908074	37.0	35.0	39.0	31.0	39.0
13	35.154432394803784	37.0	35.0	39.0	30.0	39.0
14	35.867152603703275	37.0	35.0	40.0	30.0	41.0
15	36.23505427091512	37.0	35.0	40.0	31.0	41.0
16	36.36819679866633	37.0	35.0	40.0	32.0	41.0
17	36.17710868831569	37.0	35.0	40.0	31.0	41.0
18	36.231734150079745	36.0	35.0	40.0	31.0	41.0
19	36.14685728212017	36.0	35.0	40.0	31.0	41.0
20	35.940396625562876	36.0	34.0	40.0	31.0	41.0
21	35.80792257271663	36.0	34.0	40.0	31.0	41.0
22	35.63872323453703	35.0	34.0	40.0	30.0	41.0
23	35.538791637625245	35.0	34.0	40.0	30.0	41.0
24	35.6377879314137	35.0	34.0	40.0	30.0	41.0
25	35.50961251357367	35.0	34.0	40.0	30.0	41.0
26	35.258289931437034	35.0	34.0	40.0	30.0	41.0
27	35.26576220182757	35.0	34.0	40.0	30.0	41.0
28	35.31920195244797	36.0	34.0	40.0	30.0	41.0
29	35.32960587635677	36.0	34.0	40.0	30.0	41.0
30	35.30492242968806	36.0	34.0	40.0	30.0	41.0
31	35.103456106001886	36.0	34.0	40.0	29.0	41.0
32	34.94856243326594	35.0	34.0	40.0	29.0	41.0
33	34.70240638002474	35.0	34.0	40.0	27.0	41.0
34	34.49337120925684	35.0	34.0	40.0	26.0	41.0
35	34.256989927072716	35.0	33.0	40.0	25.0	41.0
36	34.06290102552779	35.0	33.0	40.0	23.0	41.0
37	34.10731225988161	35.0	33.0	40.0	24.0	41.0
38	34.099120093439566	35.0	33.0	40.0	24.0	41.0
39	34.08561620898245	35.0	33.0	40.0	24.0	41.0
40	33.82650397131132	35.0	33.0	40.0	23.0	41.0
41	33.846248395249724	35.0	33.0	40.0	23.0	41.0
42	33.83835244053836	35.0	33.0	40.0	23.0	41.0
43	33.783283633426855	35.0	33.0	40.0	23.0	41.0
44	33.76098262786257	35.0	33.0	40.0	23.0	41.0
45	33.793659902265254	35.0	33.0	40.0	23.0	41.0
46	33.82270831289811	35.0	33.0	40.0	23.0	41.0
47	33.76337846439172	35.0	33.0	40.0	23.0	41.0
48	33.77869483623661	35.0	33.0	39.0	23.0	41.0
49	33.83650300770496	36.0	33.0	39.0	24.0	41.0
50	33.66498042755603	35.0	33.0	39.0	24.0	41.0
51	33.39084262838974	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	6.0
11	9.0
12	6.0
13	16.0
14	36.0
15	103.0
16	281.0
17	864.0
18	2213.0
19	4640.0
20	8258.0
21	13721.0
22	21192.0
23	32644.0
24	46653.0
25	66349.0
26	85679.0
27	97144.0
28	104826.0
29	118765.0
30	142848.0
31	176645.0
32	225960.0
33	299377.0
34	527558.0
35	763962.0
36	299823.0
37	366008.0
38	527551.0
39	695101.0
40	201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.300959328465755	24.29279719370173	29.82467549583597	14.58156798199655
2	31.85116559640104	25.552204778882587	31.132068949275848	11.464560675440525
3	27.56959895394696	25.061932233842633	34.83607673072128	12.532392081489121
4	24.430100297162376	28.081736289026598	33.91678330048574	13.571380113325294
5	22.164955581203714	32.31119040818452	32.67185141622048	12.852002594391292
6	20.381938127829514	40.31564805984557	30.16766318544064	9.134750626884273
7	86.32361704122722	4.033167935847151	8.243025844959625	1.4001891779659956
8	88.25800279402634	2.9242860346647666	7.533932555332827	1.283778615976075
9	84.13435092469481	4.628789014714658	8.995654264951995	2.2412057956385363
10	53.14554820343589	25.230909035127556	14.04503801059794	7.5785047508386185
11	47.38564520359533	19.529751454375834	23.766313790849022	9.318289551179813
12	42.70387944463433	22.59646110163109	24.4540392174825	10.245620236252082
13	18.395958384304365	46.166380681550564	24.11556708234254	11.322093851802526
14	11.476659768743117	47.10846793934724	29.6765264194505	11.738345872459137
15	10.504951337878847	25.01124567511428	54.022451596064855	10.461351390942013
16	12.293046089335384	19.825206991720332	51.63119543795045	16.250551480993835
17	12.919001323554385	20.332180606622615	32.60401007163095	34.14480799819205
18	17.07182064995465	23.57370054657654	42.89091846377812	16.463560339690687
19	30.49416586042054	21.182876498937226	30.990898457063125	17.33205918357911
20	30.19391389680251	23.371235183471946	30.751768520146932	15.683082399578604
21	18.119472496816428	32.84199491578815	32.781477843751446	16.257054743643977
22	19.110582688012347	25.420799983406955	26.61571508018026	28.852902248400436
23	13.869277074854066	33.29988078071992	29.851552767386718	22.97928937703929
24	12.137507923825837	22.768462676241658	51.39493903569362	13.699090364238883
25	12.148980456939542	27.14595784416627	45.29619660680929	15.408865092084904
26	11.405361540352853	40.4911497293044	31.033223678098437	17.07026505224432
27	11.275620370206328	45.266748277931725	30.536577503550866	12.921053848311075
28	10.14766079154861	33.25759877073212	43.39324689107316	13.201493546646109
29	11.208513613424463	26.748977950698787	41.87487549816936	20.167632937707385
30	15.215582076576025	39.185268662527335	31.66507722030245	13.934072040594186
31	29.477258673861595	29.38104927658225	28.80705532699312	12.334636722563037
32	32.20562581911942	23.956593638556008	31.718334836357602	12.119445705966971
33	31.155165254169543	27.421493088609004	25.125430868157476	16.297910789063973
34	18.515847435618777	26.826822652786703	28.44177073687367	26.21555917472085
35	18.106854870943724	26.083830296388893	36.330401175686184	19.4789136569812
36	32.4024305350003	27.76279554736082	26.018279137317364	13.816494780321516
37	16.50141321730879	41.13698204537765	29.77068329197316	12.590921445340403
38	16.35300487463827	41.07899281962023	25.5204878700108	17.047514435730697
39	18.46470716089158	37.888569943346	28.353447355764764	15.293275539997659
40	25.98794498196587	25.824758461047185	27.10747840635928	21.079818150627663
41	14.421773528307341	24.815629263039906	29.90392455696793	30.85867265168482
42	16.507030653484993	23.150383519652166	28.04157162036675	32.301014206496085
43	16.567439697902923	23.54295588627371	31.173573160408484	28.716031255414887
44	13.519786122599248	30.81762215655103	35.146396868408964	20.516194852440755
45	12.575862395283426	47.57406697625942	24.860439119307	14.989631509150156
46	17.406727873675095	40.528786551684945	26.011559819429674	16.05292575521028
47	16.871969555224368	30.067910482265535	28.298763775141804	24.76135618736829
48	17.41273420927888	27.714334357579197	39.78795474766261	15.084976685479317
49	20.69629849845931	28.253262542114566	36.7929322282252	14.257506731200925
50	17.768166680566218	41.192745902188335	26.52944422382804	14.509643193417402
51	15.697838972303016	38.48987327496097	26.742496293572398	19.069791459163614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5621.0
1	6837.0
2	8053.0
3	26102.0
4	44151.0
5	34946.5
6	25742.0
7	26791.0
8	27840.0
9	29450.5
10	31061.0
11	30913.0
12	30765.0
13	31914.0
14	33063.0
15	31147.0
16	29231.0
17	27972.5
18	26714.0
19	25931.0
20	25148.0
21	26600.0
22	28052.0
23	30349.0
24	32646.0
25	41245.5
26	58885.5
27	67926.0
28	86581.5
29	105237.0
30	119818.5
31	134400.0
32	155585.0
33	176770.0
34	204660.5
35	232551.0
36	229980.5
37	227410.0
38	275873.5
39	324337.0
40	405246.5
41	486156.0
42	570948.5
43	655741.0
44	624015.0
45	592289.0
46	502305.0
47	412321.0
48	353035.5
49	293750.0
50	243737.0
51	193724.0
52	154275.0
53	114826.0
54	89153.0
55	63480.0
56	52270.0
57	41060.0
58	35662.0
59	30264.0
60	27039.5
61	23815.0
62	20668.5
63	17522.0
64	14904.0
65	12286.0
66	10028.0
67	7770.0
68	7136.0
69	6502.0
70	5251.0
71	4000.0
72	3429.0
73	2858.0
74	2190.5
75	1168.0
76	813.0
77	641.5
78	470.0
79	386.5
80	303.0
81	220.5
82	138.0
83	122.5
84	107.0
85	114.0
86	121.0
87	79.5
88	38.0
89	19.5
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4628446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.795669664841776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.99075244426012	11.835167494074076
2	8.195140861770486	2.4250519409520574
3	2.7985485923192366	1.2421920153889014
4	1.3918779251354534	0.8237506397635816
5	0.8346824284072616	0.6174842742880894
6	0.5762162234278151	0.511530293841637
7	0.42645090854803297	0.4416738739803842
8	0.3288449683528296	0.3892385218155052
9	0.29057753748258963	0.38693603309540175
>10	3.1042642230196935	10.327606001273617
>50	0.7014708506933807	7.334960093007448
>100	1.153211790304739	42.02661285928571
>500	0.18898882706353334	17.251348670648746
>1k	0.0188253461977683	3.883759415276585
>5k	0.0	0.0
>10k+	1.4707301717006486E-4	0.5026878733082194
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23101	0.4991092042555968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016312170434742028	0.0
2	0.0	0.0	0.0	0.07674282037642872	0.0
3	0.0	0.0	0.0	0.11535189132594395	0.0
4	0.0	0.0	0.0	0.16634092738685943	0.0
5	0.0	0.0	0.0	0.31181091882675094	0.0
6	0.0	0.0	0.0	0.44764484667207954	0.0
7	0.0	0.0	0.0	0.5388201569165979	0.0
8	0.0	0.0	0.0	0.7196151796953016	0.0
9	0.0	0.0	0.0	0.7971574044506514	0.0
10	0.0	0.0	0.0	0.9243275172703754	0.0
11	0.0	0.0	0.0	1.0770353591680664	0.0
12	0.0	0.0	0.0	1.1975077596238566	0.0
13	0.0	0.0	0.0	1.2520617071042852	0.0
14	0.0	0.0	0.0	1.274142552381512	0.0
15	0.0	0.0	0.0	1.3091435008640049	0.0
16	0.0	0.0	0.0	1.3847628340051932	0.0
17	0.0	0.0	0.0	1.4848612255603717	0.0
18	0.0	0.0	0.0	1.6178000132225805	0.0
19	0.0	0.0	0.0	1.6843882374343355	0.0
20	0.0	0.0	0.0	1.7535475189728906	0.0
21	0.0	0.0	0.0	1.8554607745234577	0.0
22	0.0	0.0	0.0	1.96370444853413	0.0
23	2.16055237546252E-5	0.0	0.0	2.091220249733928	0.0
24	2.16055237546252E-5	0.0	0.0	2.185982076921714	0.0
25	2.16055237546252E-5	0.0	0.0	2.2638267790096287	0.0
26	2.16055237546252E-5	0.0	0.0	2.3474617614637827	0.0
27	2.16055237546252E-5	0.0	0.0	2.42947632963634	0.0
28	2.16055237546252E-5	0.0	0.0	2.515833608083577	0.0
29	2.16055237546252E-5	0.0	0.0	2.617185120016524	0.0
30	2.16055237546252E-5	0.0	0.0	2.755309233379843	0.0
31	2.16055237546252E-5	0.0	0.0	2.8815070976306085	0.0
32	2.16055237546252E-5	0.0	0.0	2.9922138013493083	0.0
33	2.16055237546252E-5	0.0	0.0	3.1032013768768176	0.0
34	2.16055237546252E-5	0.0	0.0	3.2228311619061776	0.0
35	2.16055237546252E-5	0.0	0.0	3.3874004363451578	0.0
36	2.16055237546252E-5	0.0	0.0	3.5191941312483714	0.0
37	2.16055237546252E-5	0.0	0.0	3.655049664617455	0.0
38	2.16055237546252E-5	0.0	0.0	3.77984316982417	0.0
39	2.16055237546252E-5	0.0	0.0	3.922979764698562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGC	35	1.2129931E-7	45.000004	20
GTCGGTT	110	0.0	45.000004	45
ATCGTCC	25	3.892643E-5	45.0	21
GACCGTA	30	2.166933E-6	44.999996	9
CTTGACG	220	0.0	43.977276	1
CGGGTAT	310	0.0	42.82258	6
TCTAGCG	190	0.0	42.63158	1
CTACGCG	85	0.0	42.352943	1
ACTACGG	340	0.0	42.352943	2
CGTTTTT	20525	0.0	41.788063	1
TGATCCG	70	0.0	41.785717	1
TGATCGA	65	0.0	41.53846	29
AACCGTA	65	0.0	41.53846	34
GGTACGC	65	0.0	41.53846	8
CTAAGCG	65	0.0	41.53846	1
TACGACC	65	0.0	41.53846	39
ATCGAAT	65	0.0	41.53846	31
GTACGCG	130	0.0	41.53846	1
CCCGCTA	60	3.6379788E-12	41.249996	33
TAAGTCG	55	6.184564E-11	40.909092	1
>>END_MODULE