FastQCFastQC Report
Thu 26 May 2016
SRR1545099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545099_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2584159
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126940.4912236437463794No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG119840.4637485541717828No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG93140.36042673844759554No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG76550.2962279023852635No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA36640.1417869411286225No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34020.13164824610250375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29520.1142344569355059No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA27830.10769461167056671No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA27480.10634020584646689No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26640.10308963186862728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA253.8921615E-545.00000436
TATGCGA502.1827873E-1145.0000041
CGTTACC253.8921615E-545.00000444
CGCACAC253.8921615E-545.00000421
TAATGCG650.045.0000041
GTACGCG1000.045.0000041
CGGCGTA351.2127202E-745.021
CTCGTTA406.8212103E-945.011
CTCCGTA207.034924E-445.022
GATCACG453.8562575E-1045.01
TAAGTCG351.2127202E-745.01
CACTACG207.034924E-445.01
CGGTCTA1400.045.031
CGCGTCA207.034924E-445.039
CGTCATT207.034924E-445.023
TGTAACG700.045.01
GCGTACA351.2127202E-745.044
ACGTTAG1200.044.9999961
TTAGCCG302.1665583E-644.99999624
TGAGTCG302.1665583E-644.99999616