FastQCFastQC Report
Thu 26 May 2016
SRR1545098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545098_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5848900
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282690.4833216502248286No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG90120.15408025440681153No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG84070.14373642907213321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60960.10422472601685788No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59580.10186530800663372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG2100.045.0000041
GGTCGAC253.892769E-545.08
TACTCGA700.045.020
CGCACCA900.045.032
ACGAGAC406.8230293E-945.011
CGGACTA750.045.06
GCGTCGA1250.044.99999641
TCGATAG4000.044.43751
CGTCGAT1300.043.26923442
CGTTTTT215000.042.9069751
GTACGCG1050.042.8571471
TACGTCG950.042.631581
TTACGCG1850.042.5675661
TACGCGG4450.042.471912
CGTATCA800.042.187529
GTCGATC750.042.013
GACCGTA700.041.7857138
TATTCGC700.041.78571324
CGTTCGA650.041.53846414
CGTAAAT1200.041.24999620