FastQCFastQC Report
Thu 26 May 2016
SRR1545097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545097_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1779873
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56830.3192924438990872No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29000.16293297330764608No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22640.12720008674776234No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA19870.11163717860768717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18740.10528841102707891No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA17870.1004004218278495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG750.045.0000041
AAGCGAA453.8562575E-1045.00000424
ATCGTAC453.8562575E-1045.00000429
TATCTCG453.8562575E-1045.0000041
GTTCGAC453.8562575E-1045.00000423
TATTACG453.8562575E-1045.0000041
CGTACTC453.8562575E-1045.00000431
TCGTACT453.8562575E-1045.00000430
TGCGTTG900.045.0000041
CGGGTTC207.0343306E-445.06
GTGCAGT207.0343306E-445.023
TATCACG207.0343306E-445.01
CATGGAC253.8916718E-545.014
CAGCGCG351.2124292E-745.01
AACCGAT253.8916718E-545.014
CCGGGTC351.2124292E-745.05
GCAAGTA253.8916718E-545.09
CATATCG253.8916718E-545.01
GGTAATC800.045.08
ATAGGCG207.0343306E-445.04