##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545097_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1779873
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.910012680680026	31.0	31.0	33.0	30.0	33.0
2	31.228659011064273	31.0	31.0	33.0	30.0	34.0
3	31.390814962640594	31.0	31.0	34.0	30.0	34.0
4	35.22314288716105	37.0	35.0	37.0	33.0	37.0
5	35.08711913715192	37.0	35.0	37.0	32.0	37.0
6	35.156313399888646	37.0	35.0	37.0	32.0	37.0
7	35.559396653581466	36.0	35.0	37.0	35.0	37.0
8	35.64303632899651	37.0	35.0	37.0	35.0	37.0
9	37.5270746845421	39.0	37.0	39.0	35.0	39.0
10	37.09493036862742	39.0	37.0	39.0	33.0	39.0
11	36.581710605194864	39.0	35.0	39.0	32.0	39.0
12	34.772836039425286	35.0	34.0	39.0	30.0	39.0
13	34.187315050006376	35.0	33.0	39.0	26.0	39.0
14	34.72897223565951	35.0	33.0	40.0	25.0	41.0
15	35.3165995551368	35.0	33.0	40.0	30.0	41.0
16	35.624055761281845	35.0	34.0	40.0	31.0	41.0
17	35.50368031876432	35.0	34.0	39.0	31.0	41.0
18	35.64717482651852	36.0	35.0	39.0	31.0	41.0
19	35.482456332558556	36.0	34.0	39.0	31.0	41.0
20	35.12573762285287	35.0	34.0	39.0	30.0	41.0
21	34.95765034921031	35.0	34.0	39.0	30.0	41.0
22	34.78110573057741	35.0	34.0	39.0	29.0	41.0
23	34.78811915232154	35.0	34.0	39.0	29.0	41.0
24	34.950609397412066	35.0	34.0	39.0	30.0	41.0
25	34.852062478615046	35.0	34.0	38.0	30.0	41.0
26	34.599785490313074	35.0	34.0	38.0	29.0	41.0
27	34.58713514953033	35.0	34.0	38.0	29.0	41.0
28	34.74544138823388	35.0	34.0	38.0	29.0	41.0
29	34.79459826628079	35.0	34.0	38.0	29.0	41.0
30	34.74853318186185	35.0	34.0	38.0	29.0	41.0
31	34.40351362147749	35.0	34.0	38.0	29.0	41.0
32	34.23418918091347	35.0	34.0	38.0	29.0	41.0
33	34.00291537654653	35.0	33.0	38.0	27.0	41.0
34	33.84323038778609	35.0	33.0	38.0	26.0	41.0
35	33.61224705358191	35.0	33.0	38.0	24.0	41.0
36	33.27923621516816	35.0	33.0	38.0	23.0	41.0
37	33.322277488337654	35.0	33.0	38.0	23.0	41.0
38	33.42359539135657	35.0	33.0	38.0	23.0	41.0
39	33.4442772040477	35.0	33.0	38.0	23.0	41.0
40	33.08670337715107	35.0	32.0	38.0	23.0	41.0
41	33.26949675622924	35.0	33.0	38.0	23.0	41.0
42	33.222897364025414	35.0	33.0	38.0	23.0	41.0
43	33.195965667213336	35.0	33.0	38.0	23.0	41.0
44	33.19236990504379	35.0	33.0	38.0	23.0	41.0
45	33.276266902189086	35.0	33.0	38.0	23.0	40.0
46	33.306339834358965	35.0	33.0	38.0	23.0	40.0
47	33.28392362825887	35.0	33.0	38.0	23.0	40.0
48	33.34041024275327	35.0	33.0	38.0	23.0	40.0
49	33.3936219044842	35.0	33.0	38.0	24.0	40.0
50	33.127494489775394	35.0	32.0	38.0	24.0	40.0
51	32.811594422748136	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	5.0
14	10.0
15	33.0
16	118.0
17	404.0
18	1012.0
19	2160.0
20	3873.0
21	6196.0
22	9431.0
23	13889.0
24	19847.0
25	27359.0
26	34982.0
27	39860.0
28	44241.0
29	50894.0
30	60895.0
31	75482.0
32	97540.0
33	132075.0
34	255067.0
35	380755.0
36	81594.0
37	97003.0
38	140687.0
39	204352.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.031202788064093	24.922901802544338	30.46661194366115	13.579283465730422
2	29.473057909187904	26.539365449107887	32.36607330972491	11.621503331979303
3	27.734675451563117	26.177654248364913	33.53728046888739	12.550389831184585
4	25.437432895493107	28.152458068637483	33.07685435983354	13.333254676035875
5	23.150528155660545	33.39654009021992	31.489999567384864	11.962932186734673
6	19.837876073180503	42.2023930920914	28.223418187702155	9.73631264702594
7	87.15048770333613	5.122668864576293	6.4656298511185915	1.2612135809689793
8	90.36397540723411	3.0171815629542107	5.800245298400504	0.8185977314111738
9	87.23959518460025	4.394470841458913	6.9956114846396344	1.370322489301203
10	69.6065393429756	15.302046831431232	10.12943058296856	4.961983242624615
11	67.33868090588486	12.414818360635843	14.914097803607337	5.332402929871963
12	58.56479647705201	21.22381765440568	14.313212234805517	5.898173633736789
13	15.698479610623902	62.276184873864594	14.795606203363947	7.229729312147552
14	7.873370740496653	58.73795489902931	25.19432566256132	8.194348697912716
15	6.287527256158164	21.033073708067935	65.84795656768769	6.831442468086206
16	6.5344549863950965	13.531864352119506	61.754630807928436	18.17904985355697
17	7.632510858920834	14.969438830748036	30.411945121927236	46.98610518840389
18	15.74842699451028	20.443705814965448	47.83139021716718	15.976476973357087
19	36.751891848463345	16.288409341565384	27.689728424443764	19.269970385527508
20	35.87081774935628	19.01708717419726	29.49626181193827	15.615833264508197
21	15.537793988672224	36.02448039832055	30.676008906253422	17.7617167067538
22	19.099845887880765	24.20346844971523	18.662848416712876	38.033837245691124
23	10.538673264890248	35.53461398650353	25.44198378198894	28.48472896661728
24	8.618592450135488	18.38383974586951	61.99672673275003	11.00084107124497
25	7.761115540266076	23.90614386532073	53.67927936431419	14.653461230099001
26	7.225122241867819	46.652204960691016	28.406184036726216	17.71648876071495
27	7.010387819805121	57.05317177124435	26.04112765348988	9.895312755460642
28	6.290392629137023	35.91222519808998	47.17859083204251	10.61879134073049
29	7.150397809281898	25.78442394485449	44.05117668507809	23.014001560785516
30	13.42556463298224	47.33697291885433	28.031044911631337	11.2064175365321
31	37.032136562552495	30.81821006330227	23.901480611257096	8.248172762888139
32	41.638869739582546	20.02227125193764	29.041004611003146	9.297854397476673
33	39.92947811444974	27.133452780057905	17.707892641778376	15.229176463713983
34	18.669141000509587	26.39856888665652	23.832711659764488	31.099578453069405
35	19.823886310989604	22.794884803578682	36.69660700510654	20.684621880325167
36	42.81687513659683	25.82476390169411	20.972844691728003	10.38551626998106
37	15.436101339814694	48.20298976387642	27.25076452083941	9.110144375469485
38	14.977079825358325	47.34135525399846	20.73715371827091	16.944411202372304
39	20.06485856013322	41.693929847803744	25.775996377269617	12.465215214793416
40	30.12754280782955	23.109513993414136	22.226473461870594	24.53646973688572
41	12.123055970847359	21.591147233538575	27.49213005647032	38.79366673914375
42	15.036466084939768	17.874646112391165	26.227994918738585	40.86089288393048
43	15.354915772080368	19.589993218617284	30.358121056951816	34.69696995235053
44	9.459888430241934	31.598827556797595	37.01174184899709	21.929542163963383
45	9.985881015106134	58.62176683392579	19.21238200703084	12.179970143937236
46	18.45002424330275	46.188913478658314	21.12476564339141	14.236296634647527
47	16.719338964072154	30.022422948154166	25.00723366217702	28.25100442559666
48	17.421523895244214	26.71291715757248	42.782546844634425	13.08301210254889
49	23.109626360981935	27.123452066523846	39.32482823212668	10.442093340367544
50	19.484648623806304	47.264327286272675	22.035448596613357	11.215575493307668
51	15.037983047105048	42.152670443340625	22.794603884659185	20.014742624895145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2256.0
1	2660.0
2	3064.0
3	6996.0
4	10928.0
5	9106.0
6	7284.0
7	7915.0
8	8546.0
9	9257.0
10	9968.0
11	10228.0
12	10488.0
13	9939.5
14	9391.0
15	8824.0
16	8257.0
17	7847.0
18	7437.0
19	6712.0
20	5987.0
21	6359.5
22	6732.0
23	7785.0
24	8838.0
25	9822.0
26	12917.5
27	15029.0
28	20633.5
29	26238.0
30	34040.5
31	41843.0
32	46411.0
33	50979.0
34	59220.0
35	67461.0
36	68880.0
37	70299.0
38	84901.0
39	99503.0
40	157882.5
41	216262.0
42	274007.5
43	331753.0
44	304660.0
45	277567.0
46	232439.0
47	187311.0
48	150842.0
49	114373.0
50	94845.0
51	75317.0
52	58153.0
53	40989.0
54	29505.0
55	18021.0
56	13832.0
57	9643.0
58	8416.5
59	7190.0
60	6820.5
61	6451.0
62	5481.0
63	4511.0
64	3791.0
65	3071.0
66	2534.0
67	1997.0
68	1703.0
69	1409.0
70	1247.5
71	1086.0
72	859.0
73	632.0
74	514.5
75	332.5
76	268.0
77	191.0
78	114.0
79	100.5
80	87.0
81	71.5
82	56.0
83	34.5
84	13.0
85	13.0
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1779873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.670116499513476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30966130035412	11.115125425118233
2	7.034101856793992	1.9231398370363588
3	2.3662962998676766	0.9704263827467646
4	1.242178974888845	0.6792292519990696
5	0.8354427002072948	0.5710299520250917
6	0.6315789183939579	0.5180254435849271
7	0.49931370388536866	0.47779735513415944
8	0.38676000366370344	0.42296434459480675
9	0.32810063961097824	0.40366565703422636
>10	3.0740844845839126	8.681466415835903
>50	0.5255490309968368	5.185038314510855
>100	1.6462535083340453	56.47390743998515
>500	0.1062630485739764	9.598156730529107
>1k	0.014003657564012395	2.660055110128629
>5k	4.118722812944822E-4	0.31997233973671285
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5683	0.3192924438990872	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2900	0.16293297330764608	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2264	0.12720008674776234	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1987	0.11163717860768717	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1874	0.10528841102707891	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1787	0.1004004218278495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010562551373047404	0.0
2	0.0	0.0	0.0	0.04685727577192305	0.0
3	0.0	0.0	0.0	0.06506082175526007	0.0
4	0.0	0.0	0.0	0.09444494073453555	0.0
5	0.0	0.0	0.0	0.17652944901124967	0.0
6	0.0	0.0	0.0	0.23260086534263963	0.0
7	0.0	0.0	0.0	0.2792334059789659	0.0
8	0.0	0.0	0.0	0.36401473588284106	0.0
9	5.618378389918831E-5	0.0	0.0	0.3954214710824873	0.0
10	5.618378389918831E-5	0.0	0.0	0.4671681631217508	0.0
11	5.618378389918831E-5	0.0	0.0	0.5429039038178567	0.0
12	5.618378389918831E-5	0.0	0.0	0.609537871522294	0.0
13	5.618378389918831E-5	0.0	0.0	0.6349329418447272	0.0
14	5.618378389918831E-5	0.0	0.0	0.645046022946581	0.0
15	5.618378389918831E-5	0.0	0.0	0.664710347311297	0.0
16	5.618378389918831E-5	0.0	0.0	0.7117923582188167	0.0
17	5.618378389918831E-5	0.0	0.0	0.7640432772450618	0.0
18	5.618378389918831E-5	0.0	0.0	0.8430376774073206	0.0
19	5.618378389918831E-5	0.0	0.0	0.8770288666663295	0.0
20	5.618378389918831E-5	0.0	0.0	0.9106267694380442	0.0
21	5.618378389918831E-5	0.0	0.0	0.9698444776677887	0.0
22	5.618378389918831E-5	0.0	0.0	1.020353699393159	0.0
23	5.618378389918831E-5	0.0	0.0	1.0954714184663736	0.0
24	5.618378389918831E-5	0.0	0.0	1.1469357645180303	0.0
25	5.618378389918831E-5	0.0	0.0	1.1906467483915988	0.0
26	5.618378389918831E-5	0.0	0.0	1.243571872824634	0.0
27	5.618378389918831E-5	0.0	0.0	1.2859906296685213	0.0
28	5.618378389918831E-5	0.0	0.0	1.3306005540844767	0.0
29	5.618378389918831E-5	0.0	0.0	1.3813345109454438	0.0
30	5.618378389918831E-5	0.0	0.0	1.4594299705653155	0.0
31	5.618378389918831E-5	0.0	0.0	1.542750522087812	0.0
32	5.618378389918831E-5	0.0	0.0	1.6038222951862295	0.0
33	5.618378389918831E-5	0.0	0.0	1.6632085547676716	0.0
34	5.618378389918831E-5	0.0	0.0	1.736416025188314	0.0
35	5.618378389918831E-5	0.0	0.0	1.8527164578596338	0.0
36	5.618378389918831E-5	0.0	0.0	1.9304748147761104	0.0
37	5.618378389918831E-5	0.0	0.0	2.003120447357761	0.0
38	5.618378389918831E-5	0.0	0.0	2.071159009659678	0.0
39	5.618378389918831E-5	0.0	0.0	2.1425685989955463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	75	0.0	45.000004	1
AAGCGAA	45	3.8562575E-10	45.000004	24
ATCGTAC	45	3.8562575E-10	45.000004	29
TATCTCG	45	3.8562575E-10	45.000004	1
GTTCGAC	45	3.8562575E-10	45.000004	23
TATTACG	45	3.8562575E-10	45.000004	1
CGTACTC	45	3.8562575E-10	45.000004	31
TCGTACT	45	3.8562575E-10	45.000004	30
TGCGTTG	90	0.0	45.000004	1
CGGGTTC	20	7.0343306E-4	45.0	6
GTGCAGT	20	7.0343306E-4	45.0	23
TATCACG	20	7.0343306E-4	45.0	1
CATGGAC	25	3.8916718E-5	45.0	14
CAGCGCG	35	1.2124292E-7	45.0	1
AACCGAT	25	3.8916718E-5	45.0	14
CCGGGTC	35	1.2124292E-7	45.0	5
GCAAGTA	25	3.8916718E-5	45.0	9
CATATCG	25	3.8916718E-5	45.0	1
GGTAATC	80	0.0	45.0	8
ATAGGCG	20	7.0343306E-4	45.0	4
>>END_MODULE