FastQCFastQC Report
Thu 26 May 2016
SRR1545096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545096_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1889142
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69220.36640972462631183No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33740.1785995970657579No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG32010.1694420006542653No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28780.1523442917472588No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20560.10883247527184298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC207.03444E-445.00000413
AATCCCG207.03444E-445.00000442
ACACGCG207.03444E-445.00000436
CATATCG207.03444E-445.00000427
AGTTTCG207.03444E-445.0000041
CAAGTCG207.03444E-445.0000041
CGAGTGT502.1827873E-1145.00000415
GATCGAC207.03444E-445.00000432
CAACGAA207.03444E-445.00000417
ACGTTGA253.891762E-545.00000416
ACGTTCA406.8193913E-945.00000437
GCGGTAT207.03444E-445.00000439
ACGCACT253.891762E-545.00000430
GACAACG207.03444E-445.00000415
ATTCGTC302.1662472E-645.00000414
ATTCGAA600.045.00000442
CCGTCGC600.045.00000420
ATCGTAC207.03444E-445.00000437
CGTTAGC207.03444E-445.00000414
ACGATTC207.03444E-445.00000442