##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545096_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1889142
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.868690124935025	31.0	31.0	33.0	30.0	33.0
2	31.185630302010118	31.0	31.0	33.0	30.0	34.0
3	31.354482616976384	31.0	31.0	34.0	28.0	34.0
4	35.18270569390761	37.0	35.0	37.0	32.0	37.0
5	35.055497680957814	37.0	35.0	37.0	32.0	37.0
6	35.123698483226775	37.0	35.0	37.0	32.0	37.0
7	35.5303301710512	36.0	35.0	37.0	35.0	37.0
8	35.59740877075413	37.0	35.0	37.0	35.0	37.0
9	37.420164815561776	39.0	37.0	39.0	35.0	39.0
10	36.979945393199664	39.0	37.0	39.0	33.0	39.0
11	36.52754636761027	39.0	35.0	39.0	32.0	39.0
12	34.89334893830109	35.0	34.0	39.0	30.0	39.0
13	34.35215669335603	35.0	33.0	39.0	27.0	39.0
14	34.93814017156995	35.0	33.0	40.0	27.0	41.0
15	35.46901715170167	35.0	33.0	40.0	30.0	41.0
16	35.701934529008405	35.0	34.0	40.0	31.0	41.0
17	35.57140066760466	35.0	34.0	40.0	31.0	41.0
18	35.70026498802102	36.0	35.0	39.0	31.0	41.0
19	35.55512767171552	36.0	34.0	39.0	31.0	41.0
20	35.23397023622364	35.0	34.0	39.0	30.0	41.0
21	35.057203217121845	35.0	34.0	39.0	29.0	41.0
22	34.88842553921304	35.0	34.0	39.0	29.0	41.0
23	34.8703178479966	35.0	34.0	39.0	29.0	41.0
24	34.98568397717059	35.0	34.0	39.0	30.0	41.0
25	34.89950146680345	35.0	34.0	39.0	30.0	41.0
26	34.651619094806	35.0	34.0	39.0	29.0	41.0
27	34.613417096226755	35.0	34.0	39.0	29.0	41.0
28	34.739731581850386	35.0	34.0	39.0	29.0	41.0
29	34.75292540211376	35.0	34.0	39.0	29.0	41.0
30	34.728216301368555	35.0	34.0	38.0	29.0	41.0
31	34.41866942770845	35.0	34.0	39.0	29.0	41.0
32	34.250153773512	35.0	34.0	39.0	27.0	41.0
33	34.02739285876869	35.0	33.0	39.0	27.0	41.0
34	33.875858458495976	35.0	33.0	39.0	25.0	41.0
35	33.666762477357445	35.0	33.0	38.0	24.0	41.0
36	33.387896727720836	35.0	33.0	38.0	23.0	41.0
37	33.42753323995761	35.0	33.0	38.0	23.0	41.0
38	33.487028502886496	35.0	33.0	38.0	23.0	41.0
39	33.504177028513475	35.0	33.0	38.0	23.0	41.0
40	33.15035238219255	35.0	32.0	38.0	23.0	41.0
41	33.28481077653242	35.0	32.0	38.0	23.0	41.0
42	33.2639584530967	35.0	32.0	38.0	23.0	41.0
43	33.23585098420341	35.0	33.0	38.0	23.0	41.0
44	33.21029229142118	35.0	33.0	38.0	23.0	41.0
45	33.28865326163941	35.0	33.0	38.0	23.0	41.0
46	33.32696536311193	35.0	33.0	38.0	23.0	41.0
47	33.257319460368784	35.0	33.0	38.0	23.0	41.0
48	33.311664766333074	35.0	33.0	38.0	23.0	41.0
49	33.399248441885256	35.0	33.0	38.0	24.0	40.0
50	33.14867490109267	35.0	32.0	38.0	24.0	40.0
51	32.84525514757493	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	3.0
11	4.0
12	6.0
13	9.0
14	12.0
15	48.0
16	172.0
17	513.0
18	1209.0
19	2593.0
20	4484.0
21	7344.0
22	10964.0
23	15700.0
24	21873.0
25	29509.0
26	37225.0
27	42925.0
28	47654.0
29	54705.0
30	64747.0
31	79606.0
32	102391.0
33	136376.0
34	256168.0
35	376755.0
36	94495.0
37	113481.0
38	162675.0
39	225382.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.735381458884508	25.30259768720403	30.53841373491246	13.423607118998996
2	29.702743361801282	27.486657964303372	30.74337450546333	12.067224168432018
3	28.485100643572586	25.45076018637032	33.69275575896359	12.371383411093502
4	25.856605803057686	27.80950293837097	32.66424652037803	13.669644738193318
5	23.031937249820288	32.797481608052756	31.594501630899106	12.576079511227848
6	20.31170764294055	41.20733115880119	28.84182343095437	9.639137767303888
7	86.1759994748939	5.468620146076896	7.099889791238562	1.255490587790648
8	88.97256002989718	3.46432401587599	6.406876772630114	1.1562391815967248
9	84.54637078631463	5.577135016848919	8.026024512715296	1.8504696841211514
10	65.59882740418666	16.57186172347023	11.867768542544711	5.961542329798395
11	63.13516929907863	14.23863319962184	16.224720005166365	6.401477496133165
12	55.13450021226567	20.739044497449107	17.12655798240683	6.999897307878392
13	16.500347776927306	57.86304047022405	18.06799065395825	7.568621098890397
14	8.748627683890358	56.57520715753501	26.297387914725306	8.378777243849324
15	8.204888780197571	20.82760321881574	62.693063835328424	8.274444165658272
16	8.769271976378695	15.006177407521509	59.95864789412336	16.26590272197643
17	8.970897899681443	15.339132791500058	31.291877476653422	44.39809183216508
18	16.042679692685883	21.50399493526691	46.575376546601575	15.877948825445626
19	37.43757748226443	17.70142212708203	27.35299940396222	17.508000986691314
20	36.09082853485868	20.247975006643227	27.48962227296836	16.171574185529728
21	15.880701397777402	36.623768885557574	30.213345529346125	17.2821841873189
22	18.255324374769074	24.91977839675366	20.529954868400576	36.294942360076696
23	12.087921395003658	36.12692957967162	25.457588683116462	26.327560342208262
24	9.680691022697076	19.78289615073933	58.82300007093168	11.713412755631921
25	8.587443400231427	24.679457658556107	51.71755220094625	15.015546740266217
26	8.237125636929358	45.722608464583395	29.17514935351604	16.865116544971208
27	9.610818032736553	52.38097506698808	26.747804029554157	11.260402870721206
28	8.024701160632711	34.62836568135164	46.02512675066247	11.32180640735318
29	8.488086125870899	26.204488598527796	43.87118596696278	21.436239308638523
30	14.168283802911585	45.22111095936674	28.458315997421053	12.152289240300624
31	37.20408524081302	28.385108160212415	24.741125865604594	9.669680733369963
32	40.198936871870934	20.23087729773622	29.050542521419775	10.519643308973068
33	38.770034227178265	25.53476657657286	20.14898827086582	15.546210925383058
34	18.73268393799937	25.446154921122922	24.501916743156418	31.31924439772129
35	19.349842415234004	22.90341329555957	37.756981740917304	19.989762548289118
36	41.02031504249019	25.186936715186047	21.999775559486793	11.79297268283697
37	15.049742158080228	47.92916572708668	26.35519193369265	10.665900181140433
38	16.008801879371692	47.133142982369776	20.34749108325367	16.510564055004863
39	20.541706234893937	41.98810888752672	24.336868271416336	13.133316606163008
40	30.147813134216484	22.29424786490375	23.567047897934618	23.99089110294515
41	12.310562149377866	22.830364260600845	26.165476179133172	38.69359741088812
42	15.350513619410295	19.998814276534002	25.23394218115949	39.41672992289622
43	16.735586843127727	20.270471992047185	29.057794490832343	33.936146673992745
44	10.873084183190041	31.710639009666824	37.24055682420909	20.17571998293405
45	10.326910311665296	55.83661789320231	20.529160857151023	13.30731093798137
46	17.2791140104873	44.89318431330202	22.562782469502025	15.264919206708655
47	17.443421405061134	28.379126608799126	25.221979078332918	28.955472907806822
48	17.688241540339476	25.92097364835465	42.33625635341335	14.054528457892527
49	21.431475241141214	27.265393496095054	39.684682252578156	11.618449010185577
50	17.49164435495055	47.996921353715074	22.714226881833127	11.797207409501244
51	16.24409387965542	41.91119566448684	23.824307542789267	18.020402913068473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1866.0
1	2923.5
2	3981.0
3	8750.5
4	13520.0
5	10875.0
6	8230.0
7	8883.5
8	9537.0
9	9966.0
10	10395.0
11	10339.0
12	10283.0
13	9970.0
14	9657.0
15	9119.5
16	8582.0
17	8333.5
18	8085.0
19	7695.5
20	7306.0
21	7675.0
22	8044.0
23	8495.0
24	8946.0
25	10761.0
26	18031.5
27	23487.0
28	26975.5
29	30464.0
30	38928.5
31	47393.0
32	51243.5
33	55094.0
34	62871.0
35	70648.0
36	70703.5
37	70759.0
38	92961.0
39	115163.0
40	171905.0
41	228647.0
42	272749.0
43	316851.0
44	299025.0
45	281199.0
46	234290.5
47	187382.0
48	157983.0
49	128584.0
50	106250.0
51	83916.0
52	64753.5
53	45591.0
54	33637.5
55	21684.0
56	18495.0
57	15306.0
58	13436.0
59	11566.0
60	10608.0
61	9650.0
62	8458.5
63	7267.0
64	6168.0
65	5069.0
66	4406.0
67	3743.0
68	3306.5
69	2870.0
70	2577.5
71	2285.0
72	1929.0
73	1573.0
74	1237.5
75	695.0
76	488.0
77	402.0
78	316.0
79	226.5
80	137.0
81	92.0
82	47.0
83	39.0
84	31.0
85	20.5
86	10.0
87	9.0
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1889142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.530618194779787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43672484932422	12.492320624734102
2	6.955602855423253	2.1604962452419727
3	2.5458148970698424	1.18614237482923
4	1.4180739896605237	0.8809426282146279
5	0.9594809281271294	0.7450665979957698
6	0.7375809109001326	0.687305250896871
7	0.5773409916395237	0.6276523756544296
8	0.47201965400714746	0.5864605621453652
9	0.392032665317124	0.5479658680429927
>10	3.295062484210715	10.3167449979163
>50	0.5998755211502197	6.782823630233424
>100	1.5381861852954815	54.13891779213781
>500	0.06296468478183936	6.411654056534305
>1k	0.008897183719172953	2.0676326108391465
>5k	3.4219937381434434E-4	0.36787438458369287
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6922	0.36640972462631183	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3374	0.1785995970657579	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3201	0.1694420006542653	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2878	0.1523442917472588	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2056	0.10883247527184298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012598311826215286	0.0
2	0.0	0.0	0.0	0.0432471460588987	0.0
3	0.0	0.0	0.0	0.059550843716353774	0.0
4	0.0	0.0	0.0	0.09538721811277288	0.0
5	0.0	0.0	0.0	0.1703418800704235	0.0
6	0.0	0.0	0.0	0.24969007094225845	0.0
7	0.0	0.0	0.0	0.3104054644912876	0.0
8	0.0	0.0	0.0	0.47116627548379103	0.0
9	0.0	0.0	0.0	0.5296584375340763	0.0
10	0.0	0.0	0.0	0.610965189488138	0.0
11	0.0	0.0	0.0	0.6852317083628441	0.0
12	0.0	0.0	0.0	0.7598158317373708	0.0
13	0.0	0.0	0.0	0.7886119730544342	0.0
14	0.0	0.0	0.0	0.7983518443822646	0.0
15	0.0	0.0	0.0	0.8156083555391813	0.0
16	0.0	0.0	0.0	0.8568969405158532	0.0
17	0.0	0.0	0.0	0.9042729450724191	0.0
18	0.0	0.0	0.0	0.9810273658623863	0.0
19	0.0	0.0	0.0	1.015593322259523	0.0
20	0.0	0.0	0.0	1.0559290937367334	0.0
21	0.0	0.0	0.0	1.1135213763708605	0.0
22	0.0	0.0	0.0	1.169313900172671	0.0
23	0.0	0.0	0.0	1.240457308132475	0.0
24	0.0	0.0	0.0	1.2960380956010717	0.0
25	0.0	0.0	0.0	1.3397616484097012	0.0
26	0.0	0.0	0.0	1.393701479295892	0.0
27	0.0	0.0	0.0	1.4384307796872866	0.0
28	0.0	0.0	0.0	1.4905179176578573	0.0
29	0.0	0.0	0.0	1.5505981022072455	0.0
30	0.0	0.0	0.0	1.6401096370733381	0.0
31	0.0	0.0	0.0	1.7256511156916738	0.0
32	0.0	0.0	0.0	1.7989118869836147	0.0
33	0.0	0.0	0.0	1.8729666695251073	0.0
34	0.0	0.0	0.0	1.9474978588163303	0.0
35	0.0	0.0	0.0	2.0662819417492173	0.0
36	0.0	0.0	0.0	2.1549994653657585	0.0
37	0.0	0.0	0.0	2.2419701642332868	0.0
38	0.0	0.0	0.0	2.322747575354314	0.0
39	0.0	0.0	0.0	2.4051130089744444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	20	7.03444E-4	45.000004	13
AATCCCG	20	7.03444E-4	45.000004	42
ACACGCG	20	7.03444E-4	45.000004	36
CATATCG	20	7.03444E-4	45.000004	27
AGTTTCG	20	7.03444E-4	45.000004	1
CAAGTCG	20	7.03444E-4	45.000004	1
CGAGTGT	50	2.1827873E-11	45.000004	15
GATCGAC	20	7.03444E-4	45.000004	32
CAACGAA	20	7.03444E-4	45.000004	17
ACGTTGA	25	3.891762E-5	45.000004	16
ACGTTCA	40	6.8193913E-9	45.000004	37
GCGGTAT	20	7.03444E-4	45.000004	39
ACGCACT	25	3.891762E-5	45.000004	30
GACAACG	20	7.03444E-4	45.000004	15
ATTCGTC	30	2.1662472E-6	45.000004	14
ATTCGAA	60	0.0	45.000004	42
CCGTCGC	60	0.0	45.000004	20
ATCGTAC	20	7.03444E-4	45.000004	37
CGTTAGC	20	7.03444E-4	45.000004	14
ACGATTC	20	7.03444E-4	45.000004	42
>>END_MODULE