FastQCFastQC Report
Thu 26 May 2016
SRR1545095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545095_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3638079
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113120.3109333249772751No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG64710.17786859493705331No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG38550.10596251483269055No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37990.10442324094666444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37160.1021418171513043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG700.045.0000041
ACTTACG302.1668038E-645.0000041
CCGTATC302.1668038E-645.00000438
CACTACG302.1668038E-645.0000041
TGCGAGC700.045.00000418
TCGAGTA302.1668038E-645.0000041
TCGACAG600.045.0000041
TATGTCG302.1668038E-645.00000424
GTGCACG453.8562575E-1045.01
CGGGTAC453.8562575E-1045.06
AGTTTCG253.8924773E-545.01
TGCCATA502.1827873E-1145.03
CTCCGAC253.8924773E-545.039
TGGACCG253.8924773E-545.044
CATTGCG406.8212103E-945.01
ATTACCG1550.045.01
TTCACGG1450.045.02
GGTCCGT207.035303E-445.08
TTGTACG1800.045.01
TAGCCGA253.8924773E-545.019