FastQCFastQC Report
Thu 26 May 2016
SRR1545094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545094_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2817269
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276150.9802045882022625No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG59420.21091347684583903No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37480.1330366393837436No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37460.13296564864767974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC207.035032E-445.09
CACGCTA207.035032E-445.023
GCACGAC253.892253E-545.027
ACTCGAT207.035032E-445.043
CCGGTCA253.892253E-545.045
CGATCTA253.892253E-545.010
TAATGCG1250.043.1999971
CGTTTTT182250.042.5802461
TATTGCG2150.041.8604621
TAAGACG1350.041.6666641
TACGGGC2250.041.04
ATACCGG900.040.02
TAGTCGT451.9299478E-840.013
CATACGA9900.039.54545218
TATAGCG2050.039.5121961
CGGGATC3250.039.461546
ATGTCGC403.4606092E-739.37529
AACGACC403.4606092E-739.37531
GCTACGA1950.039.23076610
TGCGAAG1550.039.193551
Produced by FastQC (version 0.11.5)