##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545094_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2817269
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.096199901393867	31.0	31.0	33.0	30.0	33.0
2	31.439509326230475	31.0	31.0	33.0	30.0	34.0
3	31.505569045767373	31.0	31.0	34.0	30.0	34.0
4	35.41301700334615	37.0	35.0	37.0	33.0	37.0
5	35.35844216508967	37.0	35.0	37.0	33.0	37.0
6	35.43640135180559	37.0	35.0	37.0	33.0	37.0
7	35.69074802583637	37.0	35.0	37.0	35.0	37.0
8	35.73264782312232	37.0	35.0	37.0	35.0	37.0
9	37.55025274476807	39.0	37.0	39.0	35.0	39.0
10	37.164472757127555	39.0	37.0	39.0	33.0	39.0
11	36.82735975868829	39.0	35.0	39.0	33.0	39.0
12	35.94243290221843	37.0	35.0	39.0	32.0	39.0
13	35.622995532198026	37.0	35.0	39.0	31.0	39.0
14	36.40153283197309	38.0	35.0	40.0	31.0	41.0
15	36.6556786022208	38.0	35.0	40.0	32.0	41.0
16	36.73558967922481	38.0	35.0	40.0	32.0	41.0
17	36.56843063264459	38.0	35.0	40.0	32.0	41.0
18	36.57883113043163	38.0	35.0	40.0	32.0	41.0
19	36.487916134384044	37.0	35.0	40.0	32.0	41.0
20	36.24387908999815	36.0	35.0	40.0	31.0	41.0
21	36.136989048614105	36.0	35.0	40.0	31.0	41.0
22	35.98402459970986	36.0	34.0	40.0	31.0	41.0
23	35.86757139627064	36.0	34.0	40.0	31.0	41.0
24	35.88930308039453	36.0	34.0	40.0	31.0	41.0
25	35.73664744119216	35.0	34.0	40.0	31.0	41.0
26	35.518335309833745	35.0	34.0	40.0	30.0	41.0
27	35.516626207863006	35.0	34.0	40.0	30.0	41.0
28	35.53169221682417	36.0	34.0	40.0	30.0	41.0
29	35.501724187502155	36.0	34.0	40.0	30.0	41.0
30	35.46543407817997	36.0	34.0	40.0	30.0	41.0
31	35.28882403490756	36.0	34.0	40.0	29.0	41.0
32	35.09735953506747	36.0	34.0	40.0	29.0	41.0
33	34.81926610486965	35.0	34.0	40.0	27.0	41.0
34	34.52084199272416	35.0	34.0	40.0	25.0	41.0
35	34.25325377164907	35.0	33.0	40.0	24.0	41.0
36	34.074433076855634	35.0	33.0	40.0	23.0	41.0
37	34.05982389328105	35.0	33.0	40.0	23.0	41.0
38	34.0110415441337	35.0	33.0	40.0	23.0	41.0
39	34.010161613960186	35.0	33.0	40.0	23.0	41.0
40	33.75888422440314	35.0	33.0	40.0	23.0	41.0
41	33.741539412814326	35.0	33.0	40.0	23.0	41.0
42	33.7335355622768	35.0	33.0	40.0	23.0	41.0
43	33.675737389649335	35.0	33.0	40.0	23.0	41.0
44	33.61537893612573	35.0	33.0	40.0	22.0	41.0
45	33.63448999722781	35.0	33.0	40.0	23.0	41.0
46	33.65374694429251	35.0	33.0	40.0	23.0	41.0
47	33.59806003615558	35.0	33.0	40.0	23.0	41.0
48	33.588254440736755	35.0	33.0	40.0	23.0	41.0
49	33.59885158286269	36.0	33.0	40.0	22.0	41.0
50	33.425722570333186	35.0	33.0	39.0	22.0	41.0
51	33.172699163622646	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	9.0
12	13.0
13	7.0
14	34.0
15	89.0
16	184.0
17	527.0
18	1238.0
19	2708.0
20	4993.0
21	8262.0
22	12914.0
23	19624.0
24	29497.0
25	43527.0
26	56182.0
27	62367.0
28	64048.0
29	68230.0
30	78829.0
31	97614.0
32	125426.0
33	168755.0
34	303697.0
35	441966.0
36	189505.0
37	234738.0
38	346017.0
39	456127.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.097704904998423	23.74306464877866	28.117016869883564	17.042213576339353
2	35.20004656992286	25.58477731448435	27.481791763583814	11.733384352008985
3	27.955938889754584	25.25349904464217	34.2930689259705	12.497493139632745
4	26.19678845009121	27.08708327106854	33.30289013935126	13.413238139488987
5	22.254885848671176	31.32334895957752	33.00185392307231	13.419911268678995
6	20.695041900507192	39.425734638758314	30.860702332649105	9.018521128085391
7	81.86690727793476	5.445273419045182	11.079701654332617	1.6081176486874345
8	83.42849049913231	4.241802965921962	10.778913905629885	1.5507926293158374
9	77.9953919913221	6.663971385054107	12.510413453596373	2.8302231700274274
10	49.924448109143995	23.158633414132623	18.0681716939348	8.84874678278858
11	45.11447078713463	20.29355379269782	24.631797673562588	9.960177746604955
12	40.73679155238637	22.665425275328698	25.983816241899515	10.61396693038542
13	19.186772722093632	42.329681688188096	26.548831510232073	11.934714079486197
14	12.67173990130158	43.880580803608034	30.779843884272324	12.667835410818066
15	12.401655645946482	25.006735246084062	50.742438865440256	11.849170242529201
16	13.762654542395488	20.30047538946405	49.949436848238484	15.98743321990197
17	13.939031026146242	20.334586438142754	33.81640162866947	31.909980907041536
18	17.223665897718675	24.118818614764866	41.8392776834587	16.81823780405776
19	29.3406131966809	21.656646915860716	30.95107353965844	18.051666347799944
20	28.877753597544288	23.264729069180117	31.91534070761436	15.942176625661233
21	18.475694014309603	31.229747674077274	34.05620833509332	16.238349976519814
22	19.8865639028435	25.35693254708727	27.760643374842797	26.995860175226433
23	15.76487726234165	31.1990086853616	30.110685206134026	22.92542884616272
24	13.63586508778537	23.64293221556053	48.28250337472211	14.438699321931983
25	13.54134092271629	26.917166944299602	44.05092307479335	15.49056905819075
26	12.92095288025389	37.166667435732975	32.35637065541132	17.556009028601814
27	12.982430857685227	42.624790178005725	30.858395133727022	13.534383830582028
28	12.148857634822944	33.234384079049605	40.72550402535221	13.89125426077524
29	12.718984236152103	27.146963956938443	39.946167724842745	20.18788408206671
30	15.53660655052819	37.518923468082036	32.463531171499774	14.480938809890004
31	27.54933944894861	30.082856837596978	29.442520398300626	12.925283315153788
32	29.966183562875965	24.937980718206177	32.23664477903956	12.859190939878301
33	29.912976006196075	28.183996629359854	26.242080539699973	15.660946824744105
34	19.487418489324234	28.153328631380248	28.432925645367906	23.92632723392761
35	19.414936947802996	27.153424113920256	34.18480805347306	19.246830884803686
36	31.059831347308332	28.37946962111179	26.417143694833545	14.14355533674633
37	17.021129327728378	40.11938512083866	30.00707422684877	12.852411324584198
38	17.426237963077007	39.17091339165695	26.3002219525363	17.102626692729732
39	19.613604522677814	36.943756524492336	28.188646522572036	15.253992430257812
40	24.752233457294988	26.78519516595682	28.039246518525562	20.423324858222628
41	15.115276531988957	27.17763195491804	30.05939439932786	27.64769711376514
42	16.68800529874854	25.26776818259101	28.699779822232095	29.34444669642835
43	18.120704838622085	24.839055127501137	30.435112869946035	26.605127163930742
44	14.514552923416261	31.085494498395434	33.99274261705219	20.40720996113612
45	13.876239720097727	44.86280862778811	25.45227310562108	15.808678546493073
46	17.997713388391382	39.39492465930658	26.71984819341	15.887513758892032
47	18.281179397494522	31.4220970734424	27.683547435477408	22.61317609358567
48	18.18055002912395	29.703020904287097	36.54691830989515	15.569510756693806
49	21.388514905747375	29.189402928864798	34.92687421754898	14.495207947838848
50	18.41570684233561	39.5935922341814	27.1683676638617	14.822333259621287
51	17.456409025904165	36.86843535352854	26.83027428335739	18.8448813372099
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3951.0
1	5774.0
2	7597.0
3	28453.5
4	49310.0
5	36711.5
6	24113.0
7	24541.5
8	24970.0
9	25263.5
10	25557.0
11	25141.0
12	24725.0
13	23952.0
14	23179.0
15	22063.5
16	20948.0
17	19393.0
18	17838.0
19	17599.5
20	17361.0
21	17823.5
22	18286.0
23	21469.5
24	24653.0
25	28008.5
26	39794.0
27	48224.0
28	57115.5
29	66007.0
30	73563.5
31	81120.0
32	91393.5
33	101667.0
34	116478.5
35	131290.0
36	132966.5
37	134643.0
38	150206.0
39	165769.0
40	209474.0
41	253179.0
42	296551.0
43	339923.0
44	328018.5
45	316114.0
46	269445.0
47	222776.0
48	197726.5
49	172677.0
50	146218.5
51	119760.0
52	99690.0
53	79620.0
54	67845.5
55	56071.0
56	50877.5
57	45684.0
58	42942.5
59	40201.0
60	38111.0
61	36021.0
62	31651.5
63	27282.0
64	23510.5
65	19739.0
66	16826.0
67	13913.0
68	12355.5
69	10798.0
70	9491.5
71	8185.0
72	6993.0
73	5801.0
74	4460.5
75	2410.5
76	1701.0
77	1332.0
78	963.0
79	781.5
80	600.0
81	480.0
82	360.0
83	219.5
84	79.0
85	79.5
86	80.0
87	52.5
88	25.0
89	17.0
90	9.0
91	8.0
92	7.0
93	7.0
94	7.0
95	3.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2817269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.714871136640433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71929015236825	13.324976620012835
2	6.147202333063998	2.054993896960403
3	2.3672653393590974	1.1870560528086807
4	1.4075345950991027	0.9410703750977948
5	0.972569018106164	0.8128182904566723
6	0.7907732615786592	0.7930603899352888
7	0.6357156470649056	0.7438133584165121
8	0.5497107705268609	0.735067575342384
9	0.45183107002401524	0.6797068299883597
>10	4.55591164737779	16.360492842182275
>50	0.9881852707273722	11.826617568055262
>100	1.3784894375125956	44.75808302858507
>500	0.03017184014439721	3.2343621334006354
>1k	0.0049216476831286235	1.3476389664956556
>5k	2.1398468187515754E-4	0.21252908166349554
>10k+	2.1398468187515754E-4	0.9877129905986923
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27615	0.9802045882022625	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5942	0.21091347684583903	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3748	0.1330366393837436	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3746	0.13296564864767974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004933856156440865	0.0
2	0.0	0.0	0.0	0.028964220314070116	0.0
3	0.0	0.0	0.0	0.04713784874642783	0.0
4	0.0	0.0	0.0	0.07546315243592287	0.0
5	0.0	0.0	0.0	0.14893856426205662	0.0
6	0.0	0.0	0.0	0.23725103992554492	0.0
7	0.0	0.0	0.0	0.29596037865038805	0.0
8	0.0	0.0	0.0	0.40386629746751196	0.0
9	0.0	0.0	0.0	0.4662316590996458	0.0
10	0.0	0.0	0.0	0.5583066437745207	0.0
11	0.0	0.0	0.0	0.6481454202633827	0.0
12	0.0	0.0	0.0	0.7158705824683408	0.0
13	0.0	0.0	0.0	0.7447283166783151	0.0
14	0.0	0.0	0.0	0.757897098218168	0.0
15	0.0	0.0	0.0	0.7763901849628133	0.0
16	0.0	0.0	0.0	0.8127374418275287	0.0
17	0.0	0.0	0.0	0.854018554848685	0.0
18	0.0	0.0	0.0	0.920394893068429	0.0
19	0.0	0.0	0.0	0.9509564049439369	0.0
20	0.0	0.0	0.0	0.9862033053996618	0.0
21	0.0	0.0	0.0	1.034512501291144	0.0
22	0.0	0.0	0.0	1.0851998868407666	0.0
23	0.0	0.0	0.0	1.1444061607180571	0.0
24	0.0	0.0	0.0	1.1909760835759737	0.0
25	0.0	0.0	0.0	1.2271458636005295	0.0
26	0.0	0.0	0.0	1.2702017450232832	0.0
27	0.0	0.0	0.0	1.3129381681337493	0.0
28	0.0	0.0	0.0	1.3581947623744839	0.0
29	0.0	0.0	0.0	1.4075688193069245	0.0
30	0.0	0.0	0.0	1.4769977591774162	0.0
31	0.0	0.0	0.0	1.5408539262668919	0.0
32	0.0	0.0	0.0	1.6010540704490768	0.0
33	0.0	0.0	0.0	1.658911520341153	0.0
34	0.0	0.0	0.0	1.7250038956166416	0.0
35	0.0	0.0	0.0	1.8154105979940147	0.0
36	0.0	0.0	0.0	1.884449088816155	0.0
37	0.0	0.0	0.0	1.9582084635865442	0.0
38	0.0	0.0	0.0	2.0310804541561347	0.0
39	0.0	0.0	0.0	2.122268054630211	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAC	20	7.035032E-4	45.0	9
CACGCTA	20	7.035032E-4	45.0	23
GCACGAC	25	3.892253E-5	45.0	27
ACTCGAT	20	7.035032E-4	45.0	43
CCGGTCA	25	3.892253E-5	45.0	45
CGATCTA	25	3.892253E-5	45.0	10
TAATGCG	125	0.0	43.199997	1
CGTTTTT	18225	0.0	42.580246	1
TATTGCG	215	0.0	41.860462	1
TAAGACG	135	0.0	41.666664	1
TACGGGC	225	0.0	41.0	4
ATACCGG	90	0.0	40.0	2
TAGTCGT	45	1.9299478E-8	40.0	13
CATACGA	990	0.0	39.545452	18
TATAGCG	205	0.0	39.512196	1
CGGGATC	325	0.0	39.46154	6
ATGTCGC	40	3.4606092E-7	39.375	29
AACGACC	40	3.4606092E-7	39.375	31
GCTACGA	195	0.0	39.230766	10
TGCGAAG	155	0.0	39.19355	1
>>END_MODULE