FastQCFastQC Report
Thu 26 May 2016
SRR1545093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545093_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4796635
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229200.47783498223233584No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG146780.3060061897559435No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG129610.27021026198574627No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG112320.23416415883218128No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG88380.18425416985032214No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA81010.16888923172182166No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60040.12517108347831343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56930.11868737145936682No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA56510.11781175761757982No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT56200.11716547121054656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC207.0355274E-445.00000422
ATCGTAG207.0355274E-445.0000041
ACTACGC750.045.00000412
AACCGGT502.1827873E-1145.031
TACGCGA253.892665E-545.025
CCGAATA453.8562575E-1045.019
CGATATA453.8562575E-1045.010
CGGCATA650.044.9999965
CGCGATT302.1669493E-644.99999627
CGTAGAT302.1669493E-644.99999644
ATACCGG302.1669493E-644.9999962
GTACGTC551.8189894E-1244.99999640
TACGTCC551.8189894E-1244.99999641
TACCGCG302.1669493E-644.9999961
CGGGTAT4100.044.4512186
AATACGG2750.044.181822
TCGACAG2100.043.928571
TCGTTAG1850.043.7837831
GCTACGA6900.043.69565210
CGGTCTA2650.043.30188831