##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545093_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4796635
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88833484307228	31.0	31.0	33.0	30.0	33.0
2	31.20429801308626	31.0	31.0	33.0	30.0	34.0
3	31.361955204012812	31.0	31.0	34.0	30.0	34.0
4	35.209899856878835	37.0	35.0	37.0	33.0	37.0
5	35.06563643053933	37.0	35.0	37.0	32.0	37.0
6	35.14966471286642	37.0	35.0	37.0	32.0	37.0
7	35.55071440707913	36.0	35.0	37.0	35.0	37.0
8	35.61258694897569	37.0	35.0	37.0	35.0	37.0
9	37.41609961983766	39.0	37.0	39.0	35.0	39.0
10	36.96396974128738	39.0	37.0	39.0	33.0	39.0
11	36.53444258318592	39.0	35.0	39.0	32.0	39.0
12	35.13772738596954	35.0	35.0	39.0	31.0	39.0
13	34.67097850889217	35.0	33.0	39.0	29.0	39.0
14	35.3494739541366	36.0	33.0	40.0	28.0	41.0
15	35.79017894836693	36.0	34.0	40.0	30.0	41.0
16	35.98166089352223	35.0	34.0	40.0	31.0	41.0
17	35.83120729428026	35.0	34.0	40.0	31.0	41.0
18	35.930531508025936	36.0	35.0	40.0	31.0	41.0
19	35.79790061157457	36.0	35.0	40.0	31.0	41.0
20	35.51869946326956	35.0	34.0	40.0	30.0	41.0
21	35.382654923712145	35.0	34.0	40.0	30.0	41.0
22	35.20555868853894	35.0	34.0	40.0	30.0	41.0
23	35.13911856958055	35.0	34.0	39.0	29.0	41.0
24	35.24915404236511	35.0	34.0	39.0	30.0	41.0
25	35.170160331148814	35.0	34.0	39.0	30.0	41.0
26	34.92353243471725	35.0	34.0	39.0	29.0	41.0
27	34.89942490933748	35.0	34.0	39.0	29.0	41.0
28	34.999404374108096	36.0	34.0	39.0	29.0	41.0
29	34.99541011563315	36.0	34.0	39.0	29.0	41.0
30	34.97059813806971	36.0	34.0	39.0	29.0	41.0
31	34.71901280793723	35.0	34.0	39.0	29.0	41.0
32	34.57417710540827	35.0	34.0	39.0	29.0	41.0
33	34.34346307359222	35.0	33.0	39.0	27.0	41.0
34	34.17494639471212	35.0	34.0	39.0	26.0	41.0
35	33.95906359354005	35.0	33.0	39.0	24.0	41.0
36	33.699562714277825	35.0	33.0	39.0	23.0	41.0
37	33.753980029750025	35.0	33.0	39.0	23.0	41.0
38	33.812732467657014	35.0	33.0	39.0	24.0	41.0
39	33.82046789051074	35.0	33.0	39.0	24.0	41.0
40	33.5134885185135	35.0	33.0	39.0	23.0	41.0
41	33.59749449353557	35.0	33.0	39.0	23.0	41.0
42	33.57116603618996	35.0	33.0	39.0	23.0	41.0
43	33.53751327753727	35.0	33.0	39.0	23.0	41.0
44	33.508549222527876	35.0	33.0	39.0	23.0	41.0
45	33.60110723455089	35.0	33.0	39.0	23.0	41.0
46	33.61832930794192	35.0	33.0	39.0	23.0	41.0
47	33.55073233631494	35.0	33.0	39.0	23.0	41.0
48	33.57415771681606	35.0	33.0	39.0	23.0	41.0
49	33.633452201387016	35.0	33.0	39.0	24.0	41.0
50	33.44306268873908	35.0	33.0	39.0	24.0	41.0
51	33.15973948403412	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	10.0
12	6.0
13	16.0
14	43.0
15	135.0
16	351.0
17	1112.0
18	2573.0
19	5492.0
20	9871.0
21	16110.0
22	24600.0
23	35826.0
24	50728.0
25	70353.0
26	90310.0
27	105632.0
28	117234.0
29	133851.0
30	159648.0
31	195670.0
32	250294.0
33	331029.0
34	605644.0
35	888194.0
36	268546.0
37	324144.0
38	460229.0
39	648705.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.72978556842453	25.464851922232985	29.110074041489504	13.695288467852984
2	30.139462352253194	27.37952752294056	30.34585704353156	12.135153081274686
3	28.899885023563392	26.20795620262955	32.91565858148473	11.976500192322327
4	26.92450019649192	28.255725107288754	32.367691100114975	12.452083596104352
5	23.234454987715345	32.815963691212694	31.47275120996282	12.476830111109143
6	20.749379512929377	42.9591786742164	27.236072788527792	9.05536902432643
7	84.98174240900131	5.955737720297667	7.695582423928442	1.3669374467725812
8	86.6537270398936	4.743742227624157	7.092659750012248	1.5098709824700023
9	81.91657276403144	7.228817702410127	8.552370568117023	2.302238965441398
10	59.71215237348682	20.665320584117826	13.008390256919695	6.614136785475651
11	57.865837196284474	16.70623259847789	18.496675273394786	6.931254931842844
12	51.26725298047485	21.677926296247264	19.380607446678766	7.674213276599116
13	16.98913926116955	54.04457499893154	19.724390119323235	9.241895620575674
14	9.595560220863169	53.99305971790641	26.06983854306196	10.341541518168466
15	9.968675957207502	23.51550618298036	57.526745312078155	8.989072547733985
16	9.703427506991881	18.10183597459469	55.68920712124229	16.50552939717114
17	9.647492460860581	19.416674397780945	30.330721432837816	40.60511170852066
18	15.678053468733811	23.729614615245897	44.59349106196323	15.998840854057065
19	34.57127757271504	19.250745574762306	28.070324300264666	18.10765255225799
20	32.96865406686146	22.079791353730272	29.310735546899025	15.640819032509249
21	16.00989026682247	36.92697067840267	30.4739051439186	16.589233910856258
22	19.36553437983086	25.71452695483396	21.749872566914096	33.17006609842108
23	13.519477717191322	36.07239241676717	25.51150963123106	24.896620234810445
24	10.91746609862956	22.615854656441442	54.35475077841028	12.111928466518716
25	9.753608519305722	27.00720400864356	47.988183382725595	15.251004089325122
26	9.03110201213976	44.608042930095785	28.3585680378015	18.002287019962953
27	11.052498261802285	51.072637380163386	26.131840342239926	11.743024015794406
28	8.949836708442481	35.77895754002546	43.28722114565732	11.983984605874744
29	9.260408598944887	27.42912479269321	41.872479352712894	21.437987255649013
30	13.977799019520976	45.39978547460876	27.80584722414776	12.816568281722498
31	33.54841466986752	31.059232149204597	24.796987888384255	10.595365292543626
32	35.59084649968155	24.351133659325757	28.86657000167826	11.191449839314437
33	34.634655336501524	29.515754273568866	21.191168392008148	14.658421997921458
34	18.202906829475246	28.386525136892843	24.544185663491177	28.866382370140737
35	19.771965138060327	24.12816484889928	35.621638919784395	20.478231093256003
36	37.26595832286593	27.966188796937853	22.85539758601603	11.912455294180191
37	14.793349921351115	45.72918306270959	28.220429530285294	11.257037485654005
38	15.153706713143695	45.122799629323474	22.653047396768777	17.070446260764058
39	19.393011976104084	40.49547234675976	26.41689434363882	13.694621333497336
40	27.253126410494023	26.020324665103768	24.502489766263224	22.224059158138985
41	12.445349708702038	25.361383553261817	28.264481245706623	33.92878549232952
42	15.236222893757812	22.763103717501956	26.46503225698849	35.53564113175174
43	17.085706959149487	22.704375046256388	28.77471394008508	31.435204054509047
44	11.115250587130353	32.80973015457712	35.312213666455754	20.762805591836777
45	11.270505260458634	53.56086089519007	20.73372270352028	14.43491114083102
46	17.74306362689677	45.046871400471375	22.641018130418512	14.569046842213343
47	17.561665626006565	30.45587166836751	25.872512709430673	26.109949996195247
48	17.70168044889803	28.413648318039623	39.596029299706984	14.288641933355361
49	21.13765170791607	28.829147933916172	37.48185967871226	12.551340679455494
50	17.978332727005494	45.46683247735131	23.791407934937723	12.763426860705474
51	16.186347303891164	40.64415991627464	24.094933218808603	19.074559561025595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7505.0
1	10126.0
2	12747.0
3	27829.5
4	42912.0
5	33690.0
6	24468.0
7	25699.5
8	26931.0
9	27896.5
10	28862.0
11	28744.0
12	28626.0
13	27665.0
14	26704.0
15	25211.5
16	23719.0
17	22270.0
18	20821.0
19	20146.0
20	19471.0
21	22365.0
22	25259.0
23	25528.5
24	25798.0
25	35612.5
26	63079.0
27	80731.0
28	96733.0
29	112735.0
30	128873.5
31	145012.0
32	164883.5
33	184755.0
34	195380.5
35	206006.0
36	200377.5
37	194749.0
38	239960.0
39	285171.0
40	417182.5
41	549194.0
42	643072.0
43	736950.0
44	703321.5
45	669693.0
46	573068.0
47	476443.0
48	383613.0
49	290783.0
50	237222.5
51	183662.0
52	142608.5
53	101555.0
54	79540.0
55	57525.0
56	48090.5
57	38656.0
58	35572.5
59	32489.0
60	30276.0
61	28063.0
62	24629.0
63	21195.0
64	17415.0
65	13635.0
66	11179.5
67	8724.0
68	7567.5
69	6411.0
70	5811.0
71	5211.0
72	4459.5
73	3708.0
74	2962.5
75	1633.5
76	1050.0
77	743.0
78	436.0
79	381.0
80	326.0
81	239.0
82	152.0
83	123.5
84	95.0
85	58.0
86	21.0
87	17.0
88	13.0
89	11.5
90	10.0
91	7.5
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4796635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.166650357353122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37991086110306	10.451675317060014
2	8.791542609188689	2.3151033527391887
3	3.022894627171834	1.1940418986927857
4	1.4515665290833568	0.7644907583550884
5	0.9177174471949376	0.604163237702921
6	0.6044701186355307	0.4775308022125076
7	0.43669104745329596	0.40248308352026935
8	0.35611517044117497	0.3751075148918531
9	0.3035374949982606	0.35969148602900275
>10	3.0478196435506955	8.710029733786161
>50	0.5174407728048661	4.745913632805677
>100	0.7577653743682944	25.42280115756566
>500	0.3637329189940799	32.657491117324646
>1k	0.0472007645866934	9.384245063462998
>5k	9.567722551356772E-4	0.8378797733738246
>10k+	6.378481700904514E-4	1.2973520704774097
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22920	0.47783498223233584	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	14678	0.3060061897559435	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	12961	0.27021026198574627	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	11232	0.23416415883218128	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8838	0.18425416985032214	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8101	0.16888923172182166	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6004	0.12517108347831343	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5693	0.11868737145936682	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5651	0.11781175761757982	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5620	0.11716547121054656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022432392708638452	0.0
2	0.0	0.0	0.0	0.08549743726591663	0.0
3	0.0	0.0	0.0	0.1190626345344184	0.0
4	0.0	0.0	0.0	0.18031390756228063	0.0
5	0.0	0.0	0.0	0.3355060370447199	0.0
6	0.0	0.0	0.0	0.46032270539659575	0.0
7	0.0	0.0	0.0	0.5544511933886985	0.0
8	0.0	0.0	0.0	0.7693935435987937	0.0
9	0.0	0.0	0.0	0.8421320363129569	0.0
10	0.0	0.0	0.0	0.9840648704769073	0.0
11	0.0	0.0	0.0	1.115135923412976	0.0
12	0.0	0.0	0.0	1.2356787622989867	0.0
13	0.0	0.0	0.0	1.2832537810360805	0.0
14	0.0	0.0	0.0	1.300390794796769	0.0
15	0.0	0.0	0.0	1.3344146469347782	0.0
16	0.0	0.0	0.0	1.4064026134988383	0.0
17	2.0847948613976255E-5	0.0	0.0	1.4934636469108031	0.0
18	2.0847948613976255E-5	0.0	0.0	1.61907253731001	0.0
19	2.0847948613976255E-5	0.0	0.0	1.6791980211127175	0.0
20	2.0847948613976255E-5	0.0	0.0	1.7373637977457113	0.0
21	2.0847948613976255E-5	0.0	0.0	1.829907841643152	0.0
22	2.0847948613976255E-5	0.0	0.0	1.9160515653161019	0.0
23	2.0847948613976255E-5	0.0	0.0	2.031132241665251	0.0
24	2.0847948613976255E-5	0.0	0.0	2.112022282287479	0.0
25	2.0847948613976255E-5	0.0	0.0	2.185386213460061	0.0
26	2.0847948613976255E-5	0.0	0.0	2.266297102030903	0.0
27	2.0847948613976255E-5	0.0	0.0	2.3343656542555355	0.0
28	2.0847948613976255E-5	0.0	0.0	2.4101062515701113	0.0
29	2.0847948613976255E-5	0.0	0.0	2.4919552978285817	0.0
30	2.0847948613976255E-5	0.0	0.0	2.622463456152073	0.0
31	2.0847948613976255E-5	0.0	0.0	2.74634196681632	0.0
32	2.0847948613976255E-5	0.0	0.0	2.848413523230348	0.0
33	2.0847948613976255E-5	0.0	0.0	2.9514649332292326	0.0
34	2.0847948613976255E-5	0.0	0.0	3.063543504977969	0.0
35	2.0847948613976255E-5	0.0	0.0	3.2254486739141086	0.0
36	2.0847948613976255E-5	0.0	0.0	3.351474523285595	0.0
37	2.0847948613976255E-5	0.0	0.0	3.4760410162541033	0.0
38	2.0847948613976255E-5	0.0	0.0	3.590537950042061	0.0
39	2.0847948613976255E-5	0.0	0.0	3.707098830742802	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0355274E-4	45.000004	22
ATCGTAG	20	7.0355274E-4	45.000004	1
ACTACGC	75	0.0	45.000004	12
AACCGGT	50	2.1827873E-11	45.0	31
TACGCGA	25	3.892665E-5	45.0	25
CCGAATA	45	3.8562575E-10	45.0	19
CGATATA	45	3.8562575E-10	45.0	10
CGGCATA	65	0.0	44.999996	5
CGCGATT	30	2.1669493E-6	44.999996	27
CGTAGAT	30	2.1669493E-6	44.999996	44
ATACCGG	30	2.1669493E-6	44.999996	2
GTACGTC	55	1.8189894E-12	44.999996	40
TACGTCC	55	1.8189894E-12	44.999996	41
TACCGCG	30	2.1669493E-6	44.999996	1
CGGGTAT	410	0.0	44.451218	6
AATACGG	275	0.0	44.18182	2
TCGACAG	210	0.0	43.92857	1
TCGTTAG	185	0.0	43.783783	1
GCTACGA	690	0.0	43.695652	10
CGGTCTA	265	0.0	43.301888	31
>>END_MODULE