##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545092_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1504317
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9423991086985	31.0	31.0	33.0	30.0	33.0
2	31.26340658252217	31.0	31.0	33.0	30.0	34.0
3	31.446209143418574	31.0	31.0	34.0	30.0	34.0
4	35.27159235719599	37.0	35.0	37.0	33.0	37.0
5	35.14532375822383	37.0	35.0	37.0	32.0	37.0
6	35.22150384526665	37.0	35.0	37.0	32.0	37.0
7	35.58752576750778	36.0	35.0	37.0	35.0	37.0
8	35.665753960102826	37.0	35.0	37.0	35.0	37.0
9	37.562168080264996	39.0	37.0	39.0	35.0	39.0
10	37.12346533343703	39.0	37.0	39.0	33.0	39.0
11	36.589961424354044	39.0	35.0	39.0	32.0	39.0
12	34.63335254470966	35.0	33.0	38.0	30.0	39.0
13	34.02000110349082	35.0	33.0	38.0	25.0	39.0
14	34.48753155086328	35.0	33.0	39.0	25.0	41.0
15	35.11690621059258	35.0	33.0	39.0	30.0	41.0
16	35.44173934084372	35.0	34.0	39.0	31.0	41.0
17	35.35044807710077	35.0	34.0	39.0	31.0	41.0
18	35.48137792765753	36.0	35.0	39.0	31.0	41.0
19	35.322407444707466	36.0	34.0	38.0	31.0	41.0
20	34.97648766849009	35.0	34.0	38.0	30.0	41.0
21	34.79314798676077	35.0	34.0	38.0	29.0	41.0
22	34.6174283744716	35.0	34.0	38.0	29.0	41.0
23	34.641011169853165	35.0	34.0	38.0	29.0	41.0
24	34.795634829627	35.0	34.0	38.0	30.0	41.0
25	34.721350619583504	35.0	34.0	38.0	30.0	41.0
26	34.4823371669668	35.0	34.0	38.0	29.0	40.0
27	34.43063529827822	35.0	34.0	38.0	29.0	40.0
28	34.60710741153626	35.0	34.0	38.0	29.0	40.0
29	34.66962016649416	35.0	34.0	38.0	29.0	40.0
30	34.64992617912315	35.0	34.0	38.0	29.0	40.0
31	34.27957471729695	35.0	34.0	38.0	29.0	40.0
32	34.086192604351346	35.0	34.0	38.0	29.0	41.0
33	33.845000089741724	35.0	33.0	38.0	27.0	41.0
34	33.71179811170119	35.0	33.0	38.0	27.0	41.0
35	33.50297709857696	35.0	33.0	38.0	25.0	41.0
36	33.175419808457924	35.0	33.0	37.0	23.0	40.0
37	33.19291545598434	35.0	33.0	38.0	23.0	40.0
38	33.33822458963104	35.0	33.0	38.0	24.0	40.0
39	33.3798807033358	35.0	33.0	37.0	24.0	40.0
40	32.96191427737638	35.0	32.0	37.0	23.0	40.0
41	33.18191112644476	35.0	32.0	37.0	23.0	40.0
42	33.169887729780356	35.0	33.0	37.0	23.0	40.0
43	33.11967956221993	35.0	33.0	37.0	23.0	40.0
44	33.09496336211051	35.0	33.0	37.0	23.0	40.0
45	33.18553602731339	35.0	33.0	37.0	23.0	40.0
46	33.23429037895603	35.0	33.0	37.0	23.0	40.0
47	33.19462187823444	35.0	33.0	37.0	23.0	40.0
48	33.26817352991424	35.0	33.0	37.0	23.0	40.0
49	33.36868226577244	35.0	33.0	37.0	24.0	40.0
50	33.093604605944094	35.0	33.0	37.0	24.0	40.0
51	32.76880670762878	35.0	32.0	36.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	4.0
13	4.0
14	4.0
15	31.0
16	99.0
17	329.0
18	753.0
19	1583.0
20	2973.0
21	4927.0
22	7400.0
23	11339.0
24	16196.0
25	22263.0
26	28613.0
27	33578.0
28	37356.0
29	43395.0
30	52513.0
31	64775.0
32	85164.0
33	115174.0
34	229998.0
35	349217.0
36	63607.0
37	74122.0
38	105158.0
39	153652.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.512529606459275	25.41159875212472	31.81144665652253	13.264424984893477
2	28.477176020745627	26.18344404802977	32.99038699954863	12.34899293167597
3	26.159579397161636	27.20450543336278	34.12286107250001	12.513054096975571
4	24.106488193645355	28.73915537749025	33.70705775444936	13.447298674415034
5	22.18415400477426	32.776203419890884	32.22598694291163	12.813655632423218
6	20.258496048372784	40.8366720578176	29.456490885897058	9.44834100791256
7	87.44413577723313	4.9522806695663215	6.408423224626192	1.195160328574363
8	91.84028366361612	1.9339673752274287	5.290241352055451	0.9355076091010073
9	89.47668609741164	2.9781621825718916	6.251209020439176	1.2939426995772834
10	74.0562660662613	12.625463914853052	9.122212937831588	4.196057081054059
11	71.63337248731484	10.460428220913544	12.75163413030631	5.154565161465303
12	60.59048724437734	20.162040314641132	13.708347376251147	5.5391250647303725
13	16.102523603735115	63.83641213919673	13.982159345403927	6.078904911664231
14	7.033490946389624	61.851325219352034	24.542699444332545	6.572484389925794
15	5.112087412427035	19.296132397626298	69.27894851949424	6.312831670452438
16	5.899222038971839	11.620290138315262	65.46073733129387	17.019750491419032
17	7.13127618713343	11.67865549614875	30.445245250834763	50.74482306588306
18	15.245789285104136	18.74152854750694	49.75447329253077	16.258208874858155
19	38.61958616435233	14.963800847826622	27.435640227425466	18.98097276039558
20	37.835509403935475	17.832544603298377	27.809431123892104	16.522514868874048
21	15.280954745575567	35.887515729729834	31.16537272396709	17.666156800727506
22	19.043991392771602	22.768007009160968	17.991553641951796	40.196447956115634
23	9.910477645336721	35.983040808552985	24.314090713592947	29.792390832517345
24	8.571929985501727	16.281741148973257	63.53288568832234	11.613443177202678
25	6.978848208190162	21.694430096847938	55.86349153802025	15.463230156941654
26	6.485202254577992	47.19071844564676	28.55907365269421	17.765005647081033
27	7.056823794452898	58.30353575742346	25.173949373702488	9.46569107442115
28	5.455632024367205	36.20972175412496	47.83552934654065	10.499116874967179
29	6.263772861704016	24.907981495921405	45.430783538310074	23.397462104064502
30	13.267283424969603	47.16080453787333	28.480100936172363	11.0918111009847
31	38.45725335816852	29.518379437312745	23.517317161210038	8.507050043308691
32	43.60749762184433	18.494971472103288	29.73296186907414	8.164569036978243
33	42.723375458763016	25.31241752901815	17.15841807278652	14.805788939432313
34	19.199410762492214	25.40102917137811	22.541791391043244	32.85776867508643
35	18.534524305714818	21.875110099799443	37.03002758062296	22.56033801386277
36	45.613524277130416	22.339373948443047	21.171269087565985	10.875832686860548
37	14.909756387782627	48.68874047158943	26.856241071529475	9.545262069098467
38	14.875521582219706	47.74964319355561	19.16550833368233	18.20932689054235
39	19.624454154277323	42.58384369783762	25.323784813972054	12.467917333912999
40	31.622856086848717	20.63900095525079	22.309858892773267	25.428284065127233
41	11.366553725045984	20.388721260213106	26.87531949715386	41.36940551758705
42	15.401275130175355	15.622903949101152	25.979364721664382	42.99645619905911
43	15.613730350717303	16.377465653848226	30.29381440215061	37.71498959328386
44	9.329682507077964	30.618214113115787	38.09030942281447	21.961793956991777
45	8.869540130172032	59.89694991148807	19.120039193866717	12.11347076447318
46	18.85127935136012	45.35427040976071	21.28055456396491	14.513895674914263
47	16.550700417531676	29.39739429920688	23.3651550836692	30.68675019959224
48	17.66628975142872	25.830659362355142	43.27259480548315	13.230456080732983
49	24.054770370872628	24.15328684047312	40.94994605525299	10.84199673340127
50	19.206324198955407	49.338869400531934	20.011872497618523	11.442933902894136
51	14.800337960682489	43.82061759589235	21.660860044791093	19.718184398634065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2276.0
1	2328.5
2	2381.0
3	4824.0
4	7267.0
5	6163.5
6	5060.0
7	5824.0
8	6588.0
9	7377.0
10	8166.0
11	8204.0
12	8242.0
13	7831.0
14	7420.0
15	6878.0
16	6336.0
17	6098.0
18	5860.0
19	5170.5
20	4481.0
21	4534.5
22	4588.0
23	4459.0
24	4330.0
25	4780.0
26	7772.5
27	10315.0
28	13191.0
29	16067.0
30	20094.0
31	24121.0
32	30237.0
33	36353.0
34	40508.0
35	44663.0
36	46249.0
37	47835.0
38	67821.0
39	87807.0
40	136334.0
41	184861.0
42	239788.5
43	294716.0
44	271643.0
45	248570.0
46	210956.5
47	173343.0
48	138881.5
49	104420.0
50	88426.5
51	72433.0
52	54128.0
53	35823.0
54	26126.5
55	16430.0
56	12253.5
57	8077.0
58	6896.0
59	5715.0
60	5163.0
61	4611.0
62	3990.5
63	3370.0
64	2837.5
65	2305.0
66	1919.0
67	1533.0
68	1274.0
69	1015.0
70	847.0
71	679.0
72	567.5
73	456.0
74	352.0
75	195.5
76	143.0
77	115.0
78	87.0
79	65.0
80	43.0
81	37.5
82	32.0
83	23.0
84	14.0
85	10.0
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1504317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.307712007586762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27611529924066	11.485675387893648
2	7.470279048393183	2.137652024814379
3	2.5484150630104505	1.0938596639204878
4	1.363746927889742	0.7804839318191048
5	0.901865679092142	0.6451817202988516
6	0.6356106682788752	0.5456480634410346
7	0.5429606232296242	0.5437966960040501
8	0.4314024413950791	0.4937905512680495
9	0.35182687434663173	0.453045383521292
>10	3.0323339425259626	8.871993622213255
>50	0.5877080557478322	6.117187779559988
>100	1.7617259720251812	57.27644248166528
>500	0.08948449381715207	8.239848938312923
>1k	0.006524911007500672	1.3153937552676744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3573	0.2375164277210189	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2005	0.13328307796827396	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1907	0.1267684936087274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0277866965539843	0.0
2	0.0	0.0	0.0	0.097785240743806	0.0
3	0.0	0.0	0.0	0.13514438778528728	0.0
4	0.0	0.0	0.0	0.1907842562438635	0.0
5	0.0	0.0	0.0	0.3253303658736822	0.0
6	0.0	0.0	0.0	0.4328210078062004	0.0
7	0.0	0.0	0.0	0.5127908545871648	0.0
8	0.0	0.0	0.0	0.6993871637427483	0.0
9	0.0	0.0	0.0	0.7650648101430749	0.0
10	0.0	0.0	0.0	0.8731537302310617	0.0
11	0.0	0.0	0.0	0.9936070655320653	0.0
12	0.0	0.0	0.0	1.0969762357269113	0.0
13	0.0	0.0	0.0	1.1443731607101428	0.0
14	0.0	0.0	0.0	1.158931262493211	0.0
15	0.0	0.0	0.0	1.186119680891727	0.0
16	0.0	0.0	0.0	1.2488724118653183	0.0
17	0.0	0.0	0.0	1.327379800932915	0.0
18	0.0	0.0	0.0	1.4585356676817454	0.0
19	0.0	0.0	0.0	1.5065973461710531	0.0
20	0.0	0.0	0.0	1.5566532851785893	0.0
21	0.0	0.0	0.0	1.6362907552065156	0.0
22	0.0	0.0	0.0	1.7056910212408687	0.0
23	0.0	0.0	0.0	1.8018143782194844	0.0
24	0.0	0.0	0.0	1.8632376021809234	0.0
25	0.0	0.0	0.0	1.914822474252435	0.0
26	0.0	0.0	0.0	1.9777081559272414	0.0
27	0.0	0.0	0.0	2.030290158257867	0.0
28	0.0	0.0	0.0	2.089187318896217	0.0
29	0.0	0.0	0.0	2.1503446414552254	0.0
30	0.0	0.0	0.0	2.2607601988144785	0.0
31	0.0	0.0	0.0	2.3711092808231244	0.0
32	0.0	0.0	0.0	2.448752490332822	0.0
33	0.0	0.0	0.0	2.525863897037659	0.0
34	0.0	0.0	0.0	2.613345458437284	0.0
35	0.0	0.0	0.0	2.7649757331732605	0.0
36	0.0	0.0	0.0	2.8683449033681065	0.0
37	0.0	0.0	0.0	2.966861372968596	0.0
38	0.0	0.0	0.0	3.0536781808621454	0.0
39	0.0	0.0	0.0	3.1379024500819974	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGT	20	7.033981E-4	45.000004	27
CAGGTTA	20	7.033981E-4	45.000004	28
AACGTTG	20	7.033981E-4	45.000004	1
ATCCTCG	20	7.033981E-4	45.000004	1
AATCCGG	20	7.033981E-4	45.000004	2
AGTCCGA	20	7.033981E-4	45.000004	38
GTCGCCG	20	7.033981E-4	45.000004	28
TCGCAAG	20	7.033981E-4	45.000004	1
GCGACGG	85	0.0	45.000004	2
AATTTCG	20	7.033981E-4	45.000004	39
GCGAATG	45	3.8562575E-10	45.000004	1
CATGCCC	20	7.033981E-4	45.000004	23
TAGACCA	20	7.033981E-4	45.000004	3
GCGCGAG	20	7.033981E-4	45.000004	1
GCGCGAC	45	3.8562575E-10	45.000004	9
TATGGTG	20	7.033981E-4	45.000004	37
TTTCGCA	20	7.033981E-4	45.000004	13
TAGGTCG	45	3.8562575E-10	45.000004	1
CATTGCG	20	7.033981E-4	45.000004	1
GCTATCC	20	7.033981E-4	45.000004	17
>>END_MODULE