FastQCFastQC Report
Thu 26 May 2016
SRR1545091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545091_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4587335
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117270.25563862242456675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81560.17779386070561665No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC74690.1628178452195011No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG59170.12898556569337097No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG56230.12257661583468396No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA55240.12041850006594243No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG47570.10369855264549024No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG47040.10254319773899224No Hit
TATAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA45970.10021068877681703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC1000.045.00000439
CTCACGA900.045.00000424
ACGCCCA253.8926366E-545.00000429
GTCGAAA253.8926366E-545.00000441
TCACACG900.045.0000041
CCGGCAT207.0354954E-445.00000431
CATTGCG453.8562575E-1045.0000041
CTATACG207.0354954E-445.0000041
CCTACGC453.8562575E-1045.00000428
TAACGCG207.0354954E-445.0000041
TCTAGCG900.045.0000041
TCGGAGC207.0354954E-445.00000439
GCCGAAT253.8926366E-545.00000435
GCGATAC2500.045.0000049
TCCGTCA502.1827873E-1145.00000418
ACCACGG453.8562575E-1045.0000042
CATCGTA207.0354954E-445.0000041
ATGCGTA453.8562575E-1045.00000429
CAGTCGT406.8230293E-945.00000417
ATCGGCA207.0354954E-445.00000411