##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545091_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4587335
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85173461279806	31.0	31.0	33.0	30.0	33.0
2	31.160805347767276	31.0	31.0	33.0	30.0	34.0
3	31.333635106221806	31.0	31.0	34.0	28.0	34.0
4	35.17250669506369	37.0	35.0	37.0	32.0	37.0
5	35.01628418242836	37.0	35.0	37.0	32.0	37.0
6	35.08395004942957	37.0	35.0	37.0	32.0	37.0
7	35.53098018784327	36.0	35.0	37.0	35.0	37.0
8	35.61387603041853	37.0	35.0	37.0	35.0	37.0
9	37.456676261925494	39.0	37.0	39.0	35.0	39.0
10	36.994764934324614	39.0	37.0	39.0	33.0	39.0
11	36.504018128172454	39.0	35.0	39.0	32.0	39.0
12	34.68180501315034	35.0	33.0	39.0	30.0	39.0
13	34.0420211299153	35.0	33.0	39.0	25.0	39.0
14	34.56602319211481	35.0	33.0	39.0	25.0	41.0
15	35.177933157268875	35.0	33.0	40.0	30.0	41.0
16	35.471019666102436	35.0	34.0	39.0	31.0	41.0
17	35.34554594334183	35.0	34.0	39.0	31.0	41.0
18	35.46457256773268	36.0	34.0	39.0	31.0	41.0
19	35.31790571214005	36.0	34.0	39.0	30.0	41.0
20	35.01096845990101	35.0	34.0	39.0	30.0	41.0
21	34.82140698248547	35.0	34.0	39.0	29.0	41.0
22	34.65300659315267	35.0	33.0	39.0	29.0	41.0
23	34.65314894159681	35.0	34.0	38.0	29.0	41.0
24	34.799251199225694	35.0	34.0	38.0	29.0	41.0
25	34.71566541357891	35.0	34.0	38.0	29.0	41.0
26	34.473029111673775	35.0	34.0	38.0	29.0	41.0
27	34.44165032638776	35.0	34.0	38.0	29.0	41.0
28	34.599520200726566	35.0	34.0	38.0	29.0	41.0
29	34.65165308398013	35.0	34.0	38.0	29.0	41.0
30	34.606461921791194	35.0	34.0	38.0	29.0	41.0
31	34.28664965606392	35.0	34.0	38.0	29.0	41.0
32	34.11826757801643	35.0	34.0	38.0	27.0	41.0
33	33.89359115913706	35.0	33.0	38.0	27.0	41.0
34	33.73184147222734	35.0	33.0	38.0	25.0	41.0
35	33.48008288036518	35.0	33.0	38.0	24.0	41.0
36	33.188181155289506	35.0	33.0	38.0	23.0	41.0
37	33.21602651648506	35.0	32.0	38.0	23.0	40.0
38	33.315648410242545	35.0	32.0	38.0	23.0	40.0
39	33.32838587109945	35.0	33.0	38.0	23.0	41.0
40	32.88254335033304	35.0	32.0	38.0	23.0	41.0
41	33.07607706871201	35.0	32.0	38.0	23.0	40.0
42	33.08445535370755	35.0	32.0	38.0	23.0	40.0
43	33.04594366881861	35.0	32.0	38.0	23.0	41.0
44	33.033645024834684	35.0	32.0	38.0	23.0	40.0
45	33.1360678912702	35.0	32.0	38.0	23.0	40.0
46	33.175278020898844	35.0	33.0	38.0	23.0	40.0
47	33.10094205023178	35.0	32.0	38.0	23.0	40.0
48	33.166816463153445	35.0	33.0	38.0	23.0	40.0
49	33.2441352114027	35.0	33.0	38.0	24.0	40.0
50	32.94685193036916	35.0	32.0	37.0	24.0	40.0
51	32.621132749188796	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	1.0
12	7.0
13	10.0
14	36.0
15	104.0
16	426.0
17	1261.0
18	2925.0
19	5965.0
20	10801.0
21	17202.0
22	26007.0
23	38039.0
24	53182.0
25	72491.0
26	92178.0
27	106760.0
28	119813.0
29	137621.0
30	165077.0
31	203191.0
32	261690.0
33	349830.0
34	664561.0
35	959646.0
36	209919.0
37	245202.0
38	347383.0
39	495743.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.44483670802329	24.319414213263258	31.745882958188144	12.489866120525315
2	28.703964284273987	26.66659400283607	31.623458936397714	13.005982776492234
3	27.186263920119195	26.112394233253077	33.69823655782715	13.00310528880058
4	25.049489518424096	28.3707642890698	32.880790262756044	13.698955929750062
5	22.491599152885065	32.79858567120125	31.744378816894773	12.96543635901891
6	20.548052409514455	41.55820318332976	28.375887089126913	9.517857318028877
7	86.39388664660417	5.773003279681994	6.704938706242296	1.1281713674715275
8	89.13292794182243	3.6981820599542	5.77915063975053	1.3897393584728388
9	85.9691520239965	5.033118357390511	7.2237148584090765	1.7740147602039094
10	69.75963168157547	15.154768509385077	10.18981609147795	4.895783717561503
11	67.87585820525425	13.267768758985337	13.6055029772188	5.250870058541615
12	58.2282741504599	21.297376363400534	14.294966467458774	6.179383018680781
13	16.511002575569474	61.144411733610035	15.511467987404451	6.833117703416036
14	8.060758588592288	60.35475935374242	24.13638855675463	7.448093500910659
15	6.847047359741549	20.631652146616716	65.11915959920086	7.4021408944408895
16	7.360700711851216	13.83293350060547	62.839578971232754	15.96678681631056
17	8.1742885575176	14.816336718377881	29.279963203036186	47.72941152106833
18	14.868305890021114	21.488969085536592	46.96746585980749	16.675259164634802
19	36.930200214285634	16.30933864651263	28.22815425513942	18.532306884062315
20	36.156613807363094	19.27814297407972	27.51087505054678	17.054368168010402
21	15.388869572420589	36.128296712579306	31.04033169585391	17.442502019146193
22	18.429676489726607	24.143865664923098	18.884406741604877	38.54205110374542
23	11.00488627928852	36.00345298522999	24.293407828292462	28.69825290718903
24	8.899437254963939	18.761546736830862	60.3238481602063	12.015167847998892
25	7.183255637532467	23.75841310913635	52.76621393467013	16.292117318661052
26	6.929884126622538	46.15926240398837	28.902554533296566	18.008298936092523
27	8.244787005963158	56.81882836112907	24.532675289683446	10.403709343224333
28	6.526142084674436	37.07928459552224	45.702003450805314	10.692569868998012
29	7.216978921312702	25.717720637363524	43.70528858258662	23.360011858737153
30	13.161497906736699	46.85990885775728	28.56098366480756	11.417609570698456
31	36.79737799833672	29.50314289233291	24.456683455644725	9.242795653685636
32	41.04986882361981	19.706321862257717	29.713177694674574	9.530631619447893
33	40.21284689258579	26.35024474994741	18.5602097950117	14.876698562455108
34	19.951823880313952	25.754779190968176	22.76236638484	31.531030543877876
35	18.48040310986662	23.217990401834616	36.64901298902304	21.652593499275724
36	43.95338469939518	23.78426690006289	21.26860148648398	10.993746914057944
37	14.903664109989787	48.364311740912754	26.463578526530107	10.268445622567352
38	15.67650934584023	47.05562161908821	19.557063087827682	17.71080594724388
39	20.763166413614876	41.1602815142125	24.932057501795704	13.144494570376919
40	30.19748939198903	21.19570513162871	23.7669583756146	24.839847100767656
41	11.72807305330873	22.259917795408445	26.3153443121115	39.696664839171326
42	16.005981686534774	18.334087220575782	25.326774695983616	40.33315639690583
43	16.73339749549575	19.401286367793066	28.81963056981886	35.045685566892324
44	10.100003596859615	31.99515622905238	36.37996788985326	21.524872284234746
45	9.638995190017734	58.44877254440759	18.979167643086893	12.933064622487784
46	18.322206684273112	45.23153421321966	22.180176507710904	14.266082594796325
47	18.17652297030847	28.835609346167217	23.721964931708715	29.2659027518156
48	18.32229388086983	26.59476580629058	41.27533742358035	13.807602889259233
49	23.946779557193885	25.204285276745647	39.345698537386085	11.503236628674383
50	18.740619553618824	48.20075272462116	20.457237154033876	12.601390567726142
51	16.083673854209472	43.04566812757298	21.809852561454527	19.06080545676302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9796.0
1	9564.5
2	9333.0
3	16875.5
4	24418.0
5	20493.5
6	16569.0
7	17944.5
8	19320.0
9	21591.5
10	23863.0
11	23917.0
12	23971.0
13	23646.0
14	23321.0
15	20931.0
16	18541.0
17	17554.0
18	16567.0
19	17327.0
20	18087.0
21	18255.5
22	18424.0
23	17744.0
24	17064.0
25	19775.5
26	33152.5
27	43818.0
28	58055.5
29	72293.0
30	77335.5
31	82378.0
32	100430.0
33	118482.0
34	125717.0
35	132952.0
36	140984.5
37	149017.0
38	197178.0
39	245339.0
40	410480.0
41	575621.0
42	707097.5
43	838574.0
44	776962.5
45	715351.0
46	614778.0
47	514205.0
48	440058.5
49	365912.0
50	293591.0
51	221270.0
52	168106.0
53	114942.0
54	80486.0
55	46030.0
56	35747.0
57	25464.0
58	21865.5
59	18267.0
60	16785.5
61	15304.0
62	13277.0
63	11250.0
64	9010.0
65	6770.0
66	5734.5
67	4699.0
68	3807.5
69	2916.0
70	2361.5
71	1807.0
72	1540.5
73	1274.0
74	977.0
75	556.5
76	433.0
77	325.5
78	218.0
79	195.0
80	172.0
81	126.5
82	81.0
83	58.0
84	35.0
85	23.0
86	11.0
87	7.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4587335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.430803336820976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.19995821462861	9.845191048504871
2	9.350909057318013	2.324786230240365
3	3.388546392456513	1.263670614069633
4	1.6364511060994131	0.8136960748097983
5	0.9281357316391077	0.5768736374941098
6	0.6079193919573014	0.45341558436366003
7	0.42027470418431806	0.3657046536609108
8	0.3291832986493845	0.3273610277821204
9	0.2630695017918291	0.29431487166207304
>10	2.3349057873520254	6.256356474462867
>50	0.4488557295153397	3.9945015661383683
>100	0.6036506861303995	19.118975699823153
>500	0.38797285990306046	35.63125911345232
>1k	0.09910943057523634	17.760211035795713
>5k	8.817564997796827E-4	0.7166044425219322
>10k+	1.7635129995593657E-4	0.257077925218107
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11727	0.25563862242456675	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8156	0.17779386070561665	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7469	0.1628178452195011	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5917	0.12898556569337097	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5623	0.12257661583468396	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	5524	0.12041850006594243	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4757	0.10369855264549024	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4704	0.10254319773899224	No Hit
TATAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	4597	0.10021068877681703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04276993068960518	0.0
2	0.0	0.0	0.0	0.16613131589474062	0.0
3	0.0	0.0	0.0	0.22326688589344357	0.0
4	0.0	0.0	0.0	0.3163492528886598	0.0
5	0.0	0.0	0.0	0.5322044280611727	0.0
6	0.0	0.0	0.0	0.7178895807696626	0.0
7	0.0	0.0	0.0	0.8537636776036631	0.0
8	0.0	0.0	0.0	1.1628320146664675	0.0
9	0.0	0.0	0.0	1.2608845876745431	0.0
10	0.0	0.0	0.0	1.4178820600631958	0.0
11	0.0	0.0	0.0	1.5898773470871432	0.0
12	0.0	0.0	0.0	1.739310514710611	0.0
13	0.0	0.0	0.0	1.8074546550448136	0.0
14	0.0	0.0	0.0	1.8296897872076052	0.0
15	0.0	0.0	0.0	1.8730046966266907	0.0
16	0.0	0.0	0.0	1.9680053887496771	0.0
17	0.0	0.0	0.0	2.0763035618719803	0.0
18	0.0	0.0	0.0	2.264321223542645	0.0
19	0.0	0.0	0.0	2.3281927306377233	0.0
20	0.0	0.0	0.0	2.394047960308109	0.0
21	0.0	0.0	0.0	2.503261697695939	0.0
22	0.0	0.0	0.0	2.606611463954562	0.0
23	0.0	0.0	0.0	2.742768949727892	0.0
24	0.0	0.0	0.0	2.8354807311870616	0.0
25	0.0	0.0	0.0	2.9078539064620306	0.0
26	0.0	0.0	0.0	2.995682678505058	0.0
27	0.0	0.0	0.0	3.0644807933146367	0.0
28	0.0	0.0	0.0	3.142390952481125	0.0
29	0.0	0.0	0.0	3.2281270062029477	0.0
30	0.0	0.0	0.0	3.3690585056465245	0.0
31	0.0	0.0	0.0	3.510186197432714	0.0
32	0.0	0.0	0.0	3.615912070951871	0.0
33	0.0	0.0	0.0	3.717626901022053	0.0
34	0.0	0.0	0.0	3.832944400180061	0.0
35	0.0	0.0	0.0	4.010978051526649	0.0
36	0.0	0.0	0.0	4.1445196393984745	0.0
37	0.0	0.0	0.0	4.274595162550805	0.0
38	0.0	0.0	0.0	4.386642789331932	0.0
39	0.0	0.0	0.0	4.4918672824199675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCC	100	0.0	45.000004	39
CTCACGA	90	0.0	45.000004	24
ACGCCCA	25	3.8926366E-5	45.000004	29
GTCGAAA	25	3.8926366E-5	45.000004	41
TCACACG	90	0.0	45.000004	1
CCGGCAT	20	7.0354954E-4	45.000004	31
CATTGCG	45	3.8562575E-10	45.000004	1
CTATACG	20	7.0354954E-4	45.000004	1
CCTACGC	45	3.8562575E-10	45.000004	28
TAACGCG	20	7.0354954E-4	45.000004	1
TCTAGCG	90	0.0	45.000004	1
TCGGAGC	20	7.0354954E-4	45.000004	39
GCCGAAT	25	3.8926366E-5	45.000004	35
GCGATAC	250	0.0	45.000004	9
TCCGTCA	50	2.1827873E-11	45.000004	18
ACCACGG	45	3.8562575E-10	45.000004	2
CATCGTA	20	7.0354954E-4	45.000004	1
ATGCGTA	45	3.8562575E-10	45.000004	29
CAGTCGT	40	6.8230293E-9	45.000004	17
ATCGGCA	20	7.0354954E-4	45.000004	11
>>END_MODULE