FastQCFastQC Report
Thu 26 May 2016
SRR1545090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545090_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3839863
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650471.6939927283864034No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG64330.16753201872045956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44960.11708751067420896No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG42330.11023830798130038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG253.892518E-545.012
ACCGGTC207.035353E-445.020
CGTACTA207.035353E-445.035
GTTCACG900.045.01
GCTCGAC207.035353E-445.020
CGTTTTT296750.043.893011
AATGTCG1050.042.8571471
ACGGGTA4050.042.777785
CATCGGC900.042.54
TATCGTG1500.042.01
CGGTCTA2900.041.89655331
CGAACGG2150.041.8604662
GCGTAAG3450.041.7391321
ACTACCG603.6379788E-1241.2500041
CGACGGT2950.041.18643628
ATTAGCG2100.040.7142871
CGGGTAC1550.040.645166
ATACGTG950.040.2631571
CTCGATT451.9303116E-840.024
TCGTACA1700.039.70588334