FastQCFastQC Report
Thu 26 May 2016
SRR1545089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545089_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1582883
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109310.6905753615396716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG207.0340926E-445.0000041
GGTCGTA207.0340926E-445.00000425
GGTCGAA207.0340926E-445.00000426
ATTACGC207.0340926E-445.00000412
TCGTGAT207.0340926E-445.00000444
CGTCCCA406.8175723E-945.00000419
TCGACTT207.0340926E-445.00000423
TACCGTA406.8175723E-945.00000420
AATCGCC207.0340926E-445.00000442
TACGTAG207.0340926E-445.0000041
AACGTAC253.8914746E-545.07
CACGGTC253.8914746E-545.020
CGTGCTA253.8914746E-545.010
CGTTAAA453.8562575E-1045.032
AAACGCG551.8189894E-1245.01
TATAGCG750.045.01
ACCGTAA453.8562575E-1045.021
ATCGGGT453.8562575E-1045.02
GACTACG351.21232E-745.01
CGTTTTT77350.042.7020031