##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545089_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1582883
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93535592965494	31.0	31.0	33.0	30.0	33.0
2	31.260550527107814	31.0	31.0	33.0	30.0	34.0
3	31.372325054978795	31.0	31.0	34.0	30.0	34.0
4	35.24457461480097	37.0	35.0	37.0	33.0	37.0
5	35.16195006200711	37.0	35.0	37.0	32.0	37.0
6	35.22576716030181	37.0	35.0	37.0	32.0	37.0
7	35.560585968767114	36.0	35.0	37.0	35.0	37.0
8	35.621497609109454	37.0	35.0	37.0	35.0	37.0
9	37.45431595386393	39.0	37.0	39.0	35.0	39.0
10	36.98472028570652	39.0	37.0	39.0	33.0	39.0
11	36.594256176862096	39.0	35.0	39.0	32.0	39.0
12	35.466377489681804	37.0	35.0	39.0	31.0	39.0
13	35.07757553780033	37.0	35.0	39.0	30.0	39.0
14	35.770213591276175	37.0	34.0	40.0	30.0	41.0
15	36.161229225407055	37.0	35.0	40.0	31.0	41.0
16	36.28679125368078	37.0	35.0	40.0	32.0	41.0
17	36.11693094183209	36.0	34.0	40.0	31.0	41.0
18	36.16382575338796	36.0	35.0	40.0	31.0	41.0
19	36.056906290610236	36.0	35.0	40.0	31.0	41.0
20	35.79411238859726	36.0	34.0	40.0	31.0	41.0
21	35.67398853863488	35.0	34.0	40.0	30.0	41.0
22	35.523274935671175	35.0	34.0	40.0	30.0	41.0
23	35.437002608531394	35.0	34.0	40.0	30.0	41.0
24	35.517817172842214	35.0	34.0	40.0	30.0	41.0
25	35.385518070508056	35.0	34.0	40.0	30.0	41.0
26	35.148292703882724	35.0	34.0	39.0	29.0	41.0
27	35.127016336646484	35.0	34.0	40.0	29.0	41.0
28	35.19687241571234	36.0	34.0	40.0	29.0	41.0
29	35.17914400495804	36.0	34.0	40.0	29.0	41.0
30	35.14245209532227	36.0	34.0	40.0	29.0	41.0
31	34.92446946489412	35.0	34.0	40.0	29.0	41.0
32	34.77362571965205	35.0	34.0	40.0	29.0	41.0
33	34.50156644553009	35.0	33.0	40.0	27.0	41.0
34	34.26918350882535	35.0	34.0	40.0	25.0	41.0
35	34.02769629846299	35.0	33.0	40.0	24.0	41.0
36	33.787134614497724	35.0	33.0	40.0	23.0	41.0
37	33.825043923018946	35.0	33.0	40.0	23.0	41.0
38	33.842306727660855	35.0	33.0	40.0	23.0	41.0
39	33.81573875011608	35.0	33.0	40.0	23.0	41.0
40	33.552538627302205	35.0	33.0	40.0	23.0	41.0
41	33.57171439708431	35.0	33.0	40.0	23.0	41.0
42	33.549110073201874	35.0	33.0	40.0	23.0	41.0
43	33.51099038905592	35.0	33.0	40.0	23.0	41.0
44	33.47187694858053	35.0	33.0	39.0	23.0	41.0
45	33.50251850578975	35.0	33.0	39.0	23.0	41.0
46	33.53577870253203	35.0	33.0	39.0	23.0	41.0
47	33.4739048937919	35.0	33.0	39.0	23.0	41.0
48	33.52430280696678	35.0	33.0	39.0	23.0	41.0
49	33.558556128279854	35.0	33.0	39.0	23.0	41.0
50	33.37379389380011	35.0	33.0	39.0	23.0	41.0
51	33.087736111892035	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	4.0
12	4.0
13	16.0
14	13.0
15	49.0
16	122.0
17	370.0
18	852.0
19	1705.0
20	3200.0
21	5346.0
22	8097.0
23	12384.0
24	17652.0
25	24188.0
26	31288.0
27	35351.0
28	37952.0
29	42248.0
30	50277.0
31	61238.0
32	78508.0
33	103885.0
34	183618.0
35	266149.0
36	98917.0
37	120680.0
38	173218.0
39	225481.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.666332887522326	24.360802409274722	28.624857301518812	15.348007401684141
2	32.57006361177674	25.236609401958326	30.04587199432933	12.1474549919356
3	28.094180049946836	25.165410204039084	34.11932530705049	12.621084438963587
4	24.6706168428115	27.84634113829007	33.67589392267148	13.807148096226948
5	22.38800972655591	31.53101018837147	32.41243983288721	13.66854025218541
6	21.085702480852973	38.686371639596864	30.874549793004284	9.353376086545879
7	84.19598921714365	4.793089571370721	9.279965733411757	1.730955478073869
8	85.95006706117887	3.503607025914107	8.967687441206962	1.5786384717000561
9	81.93644129098612	4.926516994623102	10.414225182783566	2.7228165316072004
10	55.08947913396	21.21173832810132	15.911157046983258	7.787625490955427
11	49.51755752004412	18.79936798866372	22.657265255865404	9.02580923542675
12	44.495581796001346	22.125829893934043	23.563965245694092	9.814623064370519
13	18.201850673738996	47.54577565113783	23.397117790765332	10.855255884357847
14	11.162922338543027	46.60363400200772	29.941505468186847	12.291938191262398
15	10.266709542019214	23.467116647282204	55.219431884731854	11.046741925966733
16	11.811169871683504	18.331045314151456	52.37007409897004	17.487710715194996
17	12.357514737349508	18.53920978366689	32.86294691395384	36.24032856502976
18	17.537114240281817	21.86756696483568	43.72502579154619	16.870293003336318
19	31.354244122907378	20.855173755735578	29.683179363225204	18.107402758131837
20	31.246087044967947	21.825428664026337	31.182532126505873	15.74595216449984
21	17.47507554253852	33.436141521514855	32.007798428563575	17.08098450738305
22	19.151699778189542	23.904672676375956	25.94083074996699	31.002796795467514
23	13.76519932300745	33.235937210773	28.99203541891599	24.00682804730356
24	12.272606377097992	21.395201035073345	52.46073146278025	13.871461125048409
25	11.922106687607359	26.124293456939018	46.119454185811584	15.834145669642039
26	11.631687244098268	40.58145801047835	29.752546461109254	18.03430828431413
27	10.881031636577056	45.570519109751004	30.004997210785632	13.543452042886303
28	10.705655440105174	32.24584508141158	43.82035816923929	13.228141309243954
29	11.162353755773484	25.708406748951123	42.105638888029	21.023600607246397
30	14.981081987740092	39.875657265887625	31.524818953769802	13.618441792602484
31	30.345136058697957	29.596817958118194	28.143962630213355	11.914083352970497
32	34.02847841565043	22.874337522103655	30.147964189393655	12.949219872852257
33	32.91721498051341	27.218373057263236	24.005564530037912	15.85884743218545
34	18.83973736530116	26.011650892706534	27.29740606222949	27.851205679762813
35	18.75811414994033	25.45507153718879	35.90972927247308	19.877085040397805
36	34.161400431996555	27.468107244818473	25.341354983280507	13.029137339904464
37	16.681397172122008	42.726089041325224	28.252940994375454	12.33957279217731
38	16.489532075333425	41.37406239121906	25.082460295549325	17.05394523789819
39	19.121375363814003	38.90931926112037	27.70255287345938	14.266752501606247
40	26.914939385917975	24.674975977377986	26.980263228551955	21.429821408152087
41	14.368844696670568	24.386704513220497	28.713998444610244	32.53045234549869
42	16.016092155895286	22.464768400443997	27.732182353338814	33.7869570903219
43	16.52042507247851	23.33154124467822	30.432192398301073	29.715841284542194
44	13.568596036472687	30.33913435168613	35.7465460176147	20.345723594226484
45	13.160479959668528	48.36630376344935	23.74888099752161	14.72433527936051
46	17.62120131431066	41.20058147064565	25.176845035293194	16.001372179750494
47	16.32274779626795	29.970503189433455	27.887594977013464	25.81915403728513
48	17.71950295757804	27.23239809891192	39.56306309436642	15.48503584914362
49	20.426399171638078	27.931944433037692	38.17660559877136	13.465050796552871
50	17.812118773150004	42.26225185310601	26.22171063812044	13.703918735623544
51	15.713732474225829	38.93117810981608	26.469739077367056	18.88535033859104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1561.0
1	2461.0
2	3361.0
3	11450.0
4	19539.0
5	14682.5
6	9826.0
7	10016.5
8	10207.0
9	10606.0
10	11005.0
11	11082.5
12	11160.0
13	10825.5
14	10491.0
15	10112.5
16	9734.0
17	9241.5
18	8749.0
19	8504.0
20	8259.0
21	8900.5
22	9542.0
23	10253.0
24	10964.0
25	12931.5
26	17276.0
27	19653.0
28	25475.0
29	31297.0
30	37118.0
31	42939.0
32	47282.5
33	51626.0
34	61776.5
35	71927.0
36	76602.0
37	81277.0
38	90523.0
39	99769.0
40	137450.0
41	175131.0
42	196757.5
43	218384.0
44	205910.5
45	193437.0
46	165215.0
47	136993.0
48	117329.0
49	97665.0
50	81808.0
51	65951.0
52	53415.5
53	40880.0
54	33386.0
55	25892.0
56	22830.0
57	19768.0
58	18597.0
59	17426.0
60	15645.0
61	13864.0
62	12674.0
63	11484.0
64	10078.0
65	8672.0
66	7657.0
67	6642.0
68	5578.5
69	4515.0
70	3724.5
71	2934.0
72	2790.0
73	2646.0
74	2074.5
75	992.5
76	482.0
77	428.5
78	375.0
79	267.5
80	160.0
81	166.5
82	173.0
83	115.0
84	57.0
85	47.5
86	38.0
87	26.5
88	15.0
89	11.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1582883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.363274259609337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5601605914163	14.609850490787405
2	6.320641190945466	2.3213533537183078
3	2.4259508727276033	1.3364520364870685
4	1.5281423082035845	1.1224678525301954
5	1.054712353728131	0.9683986108253886
6	0.8263495362892646	0.9104689901508459
7	0.6511996336211731	0.8370710228959903
8	0.5435305743780394	0.7984800804629544
9	0.431556966101773	0.713231903445461
>10	4.279112307200589	16.541373536201384
>50	0.9428267116472708	12.638200678285468
>100	1.423733858434098	45.052964298428
>500	0.01035693883438954	1.157353166810799
>1k	0.0013809251779186053	0.2993548232734768
>5k	0.0	0.0
>10k+	3.452312944796513E-4	0.6929791556972417
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10931	0.6905753615396716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009413203629074291	0.0
2	0.0	0.0	0.0	0.04258053185232263	0.0
3	0.0	0.0	0.0	0.06545019436054339	0.0
4	0.0	0.0	0.0	0.10676720894721846	0.0
5	0.0	0.0	0.0	0.2204205869922161	0.0
6	0.0	0.0	0.0	0.3199857475252435	0.0
7	0.0	0.0	0.0	0.39402785929219025	0.0
8	0.0	0.0	0.0	0.5412276207401305	0.0
9	0.0	0.0	0.0	0.6060460564678501	0.0
10	6.317586328237778E-5	0.0	0.0	0.7311342657669582	0.0
11	6.317586328237778E-5	0.0	0.0	0.8250135986045716	0.0
12	6.317586328237778E-5	0.0	0.0	0.9176294141765373	0.0
13	6.317586328237778E-5	0.0	0.0	0.9561035149155055	0.0
14	6.317586328237778E-5	0.0	0.0	0.9718974807360999	0.0
15	6.317586328237778E-5	0.0	0.0	1.0003897950764522	0.0
16	6.317586328237778E-5	0.0	0.0	1.0475821649483885	0.0
17	6.317586328237778E-5	0.0	0.0	1.101344824601692	0.0
18	6.317586328237778E-5	0.0	0.0	1.1826521606461122	0.0
19	6.317586328237778E-5	0.0	0.0	1.2230215372835516	0.0
20	6.317586328237778E-5	0.0	0.0	1.2673078174444985	0.0
21	6.317586328237778E-5	0.0	0.0	1.3299150979573349	0.0
22	6.317586328237778E-5	0.0	0.0	1.3920169715639121	0.0
23	6.317586328237778E-5	0.0	0.0	1.4668171936902474	0.0
24	6.317586328237778E-5	0.0	0.0	1.524623108593623	0.0
25	6.317586328237778E-5	0.0	0.0	1.572763116414795	0.0
26	6.317586328237778E-5	0.0	0.0	1.6258940174352747	0.0
27	6.317586328237778E-5	0.0	0.0	1.6793407977721664	0.0
28	6.317586328237778E-5	0.0	0.0	1.733798391921576	0.0
29	6.317586328237778E-5	0.0	0.0	1.8003225759579198	0.0
30	6.317586328237778E-5	0.0	0.0	1.8959076571041573	0.0
31	6.317586328237778E-5	0.0	0.0	1.975130189660259	0.0
32	6.317586328237778E-5	0.0	0.0	2.0467716186224756	0.0
33	6.317586328237778E-5	0.0	0.0	2.120055620030034	0.0
34	6.317586328237778E-5	0.0	0.0	2.2016156595275835	0.0
35	6.317586328237778E-5	0.0	0.0	2.3170379617444876	0.0
36	6.317586328237778E-5	0.0	0.0	2.402199025449133	0.0
37	6.317586328237778E-5	0.0	0.0	2.490139827138203	0.0
38	6.317586328237778E-5	0.0	0.0	2.6016452258315996	0.0
39	6.317586328237778E-5	0.0	0.0	2.752382835623353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	7.0340926E-4	45.000004	1
GGTCGTA	20	7.0340926E-4	45.000004	25
GGTCGAA	20	7.0340926E-4	45.000004	26
ATTACGC	20	7.0340926E-4	45.000004	12
TCGTGAT	20	7.0340926E-4	45.000004	44
CGTCCCA	40	6.8175723E-9	45.000004	19
TCGACTT	20	7.0340926E-4	45.000004	23
TACCGTA	40	6.8175723E-9	45.000004	20
AATCGCC	20	7.0340926E-4	45.000004	42
TACGTAG	20	7.0340926E-4	45.000004	1
AACGTAC	25	3.8914746E-5	45.0	7
CACGGTC	25	3.8914746E-5	45.0	20
CGTGCTA	25	3.8914746E-5	45.0	10
CGTTAAA	45	3.8562575E-10	45.0	32
AAACGCG	55	1.8189894E-12	45.0	1
TATAGCG	75	0.0	45.0	1
ACCGTAA	45	3.8562575E-10	45.0	21
ATCGGGT	45	3.8562575E-10	45.0	2
GACTACG	35	1.21232E-7	45.0	1
CGTTTTT	7735	0.0	42.702003	1
>>END_MODULE