FastQCFastQC Report
Thu 26 May 2016
SRR1545088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545088_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2329382
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.2741499676738294No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29410.1262566637846433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG1000.045.0000042
CTATGCG850.045.0000041
ATCTACG253.892043E-545.0000041
CAACGTA253.892043E-545.00000427
GCGCGTC253.892043E-545.00000438
CGTGACG253.892043E-545.00000419
TCGATAG750.045.0000041
ACGGGCC253.892043E-545.0000045
TATTGCG2700.045.0000041
ATTCCCG253.892043E-545.00000445
CCCGTAA253.892043E-545.00000420
TCGTGAC253.892043E-545.00000418
TCTACGA253.892043E-545.00000412
GTATGCG253.892043E-545.0000041
AACGCTC253.892043E-545.00000444
CGCGTCA253.892043E-545.00000439
CGACTCC253.892043E-545.00000423
CGCTTCG253.892043E-545.00000432
TGATCGC207.03478E-445.021
TTGATCG207.03478E-445.040