##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545088_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2329382
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01563590686285	31.0	31.0	33.0	30.0	33.0
2	31.3408105669229	31.0	31.0	33.0	30.0	34.0
3	31.50772307848176	31.0	31.0	34.0	30.0	34.0
4	35.3580228575648	37.0	35.0	37.0	33.0	37.0
5	35.25646373158202	37.0	35.0	37.0	33.0	37.0
6	35.33098435550717	37.0	35.0	37.0	33.0	37.0
7	35.64271596500703	36.0	35.0	37.0	35.0	37.0
8	35.70882620368836	37.0	35.0	37.0	35.0	37.0
9	37.61976438385803	39.0	37.0	39.0	35.0	39.0
10	37.18203411892081	39.0	37.0	39.0	33.0	39.0
11	36.657051956269946	39.0	35.0	39.0	32.0	39.0
12	34.92785511350221	35.0	35.0	39.0	31.0	39.0
13	34.369742274989676	35.0	33.0	39.0	27.0	39.0
14	34.927712586428505	35.0	33.0	40.0	27.0	41.0
15	35.48207936697373	35.0	34.0	40.0	31.0	41.0
16	35.750131579964126	35.0	34.0	40.0	32.0	41.0
17	35.662526369655126	35.0	34.0	40.0	31.0	41.0
18	35.810298182092936	36.0	35.0	39.0	31.0	41.0
19	35.66336478945918	36.0	35.0	39.0	31.0	41.0
20	35.344187428253505	35.0	34.0	39.0	31.0	41.0
21	35.17058172510992	35.0	34.0	39.0	30.0	41.0
22	35.000938017036276	35.0	34.0	39.0	30.0	41.0
23	35.00229417072855	35.0	34.0	39.0	30.0	41.0
24	35.147609966935434	35.0	34.0	39.0	31.0	41.0
25	35.053901850362024	35.0	34.0	39.0	31.0	41.0
26	34.836009293452086	35.0	34.0	38.0	30.0	41.0
27	34.806414319334486	35.0	34.0	38.0	30.0	41.0
28	34.99248598984624	35.0	34.0	38.0	30.0	41.0
29	35.02370371197167	35.0	34.0	38.0	30.0	41.0
30	34.98751256771109	35.0	34.0	38.0	30.0	41.0
31	34.65313975981612	35.0	34.0	38.0	29.0	41.0
32	34.48810457022506	35.0	34.0	38.0	29.0	41.0
33	34.264673205167725	35.0	33.0	38.0	28.0	41.0
34	34.11783339958839	35.0	34.0	38.0	27.0	41.0
35	33.90807905272729	35.0	33.0	38.0	27.0	41.0
36	33.59543217900713	35.0	33.0	38.0	23.0	41.0
37	33.620866822187175	35.0	33.0	38.0	24.0	41.0
38	33.71169821008319	35.0	33.0	38.0	26.0	41.0
39	33.72148836043208	35.0	33.0	38.0	25.0	41.0
40	33.40552816154671	35.0	33.0	38.0	23.0	41.0
41	33.5527066835753	35.0	33.0	38.0	23.0	41.0
42	33.538218720673555	35.0	33.0	38.0	23.0	41.0
43	33.50074354485439	35.0	33.0	38.0	24.0	41.0
44	33.48613623699333	35.0	33.0	38.0	23.0	41.0
45	33.550847392140916	35.0	33.0	38.0	24.0	41.0
46	33.58324697280223	35.0	33.0	38.0	24.0	41.0
47	33.5499372795016	35.0	33.0	38.0	24.0	41.0
48	33.60584180696854	35.0	33.0	38.0	24.0	41.0
49	33.70195828764883	35.0	33.0	38.0	24.0	40.0
50	33.42833335193627	35.0	33.0	38.0	24.0	40.0
51	33.103586702395745	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	4.0
13	2.0
14	10.0
15	34.0
16	121.0
17	363.0
18	1006.0
19	2073.0
20	3745.0
21	6457.0
22	10245.0
23	14895.0
24	22312.0
25	31653.0
26	41083.0
27	47643.0
28	53137.0
29	60985.0
30	74390.0
31	93412.0
32	123453.0
33	169478.0
34	337998.0
35	523186.0
36	109395.0
37	130644.0
38	190698.0
39	280787.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.862341170318995	24.95417239422302	32.37365103705618	12.809835398401809
2	29.500528466348584	25.151349156128106	32.80217671468226	12.545945662841046
3	26.805693527296082	26.11186142934049	34.733246844012704	12.34919819935073
4	24.764079056161677	28.308881926622597	33.57371182571171	13.353327191504013
5	21.654627708121723	32.785477006347605	33.23448021835835	12.325415067172322
6	20.56322234824516	40.86096655679489	28.913377024463998	9.662434070495951
7	87.57756349108905	4.944873790559041	6.203405023306611	1.2741576950452953
8	91.27764359817326	2.2911227098002818	5.613205562677139	0.8180281293493294
9	88.73941672082981	3.2324453438723233	6.594195370274175	1.4339425650236846
10	70.65620838488492	15.249838798445253	9.720346426648785	4.373606390021044
11	66.09817539587753	12.776521841415448	15.229532983426505	5.8957697792805135
12	57.291805294279776	21.08456234314509	15.459679863586137	6.163952498989002
13	16.605434402772925	60.049875889828286	16.436076178145104	6.908613529253682
14	7.693628610506993	58.20462251361091	25.544285995169535	8.557462880712567
15	6.137421856956051	20.447741074671306	65.39039109944183	8.024445968930817
16	6.969230465419583	13.695263378870449	62.42308045653311	16.912425699176865
17	7.568015894344509	13.469581202224454	31.612333228298322	47.350069675132715
18	15.747181011959396	19.03414725450785	49.17235558615976	16.046316147372995
19	37.241508692005006	16.045028252128677	27.16098089536195	19.552482160504375
20	36.00916466255857	18.410977675623837	29.398613022681552	16.181244639136043
21	15.301225818693542	36.00354085332504	31.24073252047109	17.454500807510318
22	18.958934172239676	23.516580792673768	19.77657593301571	37.74790910207085
23	10.462345806741874	35.94090621460971	25.538919764984875	28.057828213663537
24	8.84852720592844	17.768575527758006	61.81558026978829	11.567316996525259
25	7.354783371726921	23.59647322766296	54.56623258872955	14.482510811880575
26	7.41514272884396	45.79703972985109	28.948665354158315	17.839152187146635
27	6.850829962625279	55.553704802389646	26.80805466857733	10.787410566407743
28	6.100845632017419	35.38221725762455	47.17379974602706	11.343137364330968
29	7.280471816129771	25.275888626253657	45.00640083936426	22.43723871825231
30	14.143064555319823	46.56119090814646	27.126722881863085	12.169021654670638
31	37.00668245912435	30.61949478445356	23.49155269509252	8.88227006132957
32	41.26824196288973	19.331736915628266	29.380067331163374	10.019953790318633
33	39.556371604142214	25.799203393861546	19.188050736203852	15.456374265792386
34	18.63159413097551	25.4272592473025	23.568912269434552	32.372234352287435
35	18.043369443053994	22.348846174650618	38.16201035296057	21.445774029334817
36	42.811183395424194	24.413170531926493	21.377000423288237	11.398645649361075
37	15.079235608414592	48.05987167411786	27.019527067694348	9.8413656497732
38	15.053821142260048	46.72565513084586	20.64281427434401	17.577709452550074
39	19.254119762237366	42.046903427604406	25.61378940852123	13.085187401637
40	31.02668433086544	21.115085460435427	22.632140198559103	25.22609001014003
41	12.077666952007013	20.938686741805338	26.697553256614842	40.286093049572806
42	14.517584492367503	16.747618037745635	26.89975280997277	41.83504465991409
43	14.22574743000504	18.249132173254537	31.682609378796606	35.84251101794382
44	9.861370955901608	30.052949666478064	38.366613977441226	21.7190654001791
45	9.794657982245933	57.75922540828425	19.479973658249268	12.966142951220538
46	18.212856457206247	45.54581429752613	21.12259818269395	15.118731062573678
47	15.944357773864484	29.48129589736677	24.731624096004865	29.842722232763887
48	16.34205982530989	26.58121338621145	43.62105485489284	13.455671933585819
49	22.563581241719906	26.10752551535128	40.156487858152936	11.172405384775876
50	19.356249855111784	47.9855601185207	21.373523106128577	11.28466692023893
51	14.807103343290196	42.56261961327081	23.4642063860715	19.16607065736749
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2529.0
1	3140.0
2	3751.0
3	8359.5
4	12968.0
5	10671.5
6	8375.0
7	9380.0
8	10385.0
9	11289.0
10	12193.0
11	12374.0
12	12555.0
13	12371.0
14	12187.0
15	11205.5
16	10224.0
17	9957.0
18	9690.0
19	9185.0
20	8680.0
21	8848.0
22	9016.0
23	9830.0
24	10644.0
25	11844.0
26	15404.5
27	17765.0
28	22929.5
29	28094.0
30	34659.0
31	41224.0
32	52557.5
33	63891.0
34	72398.0
35	80905.0
36	85000.5
37	89096.0
38	115185.5
39	141275.0
40	224909.0
41	308543.0
42	376514.0
43	444485.0
44	408684.5
45	372884.0
46	305420.0
47	237956.0
48	195175.0
49	152394.0
50	122598.5
51	92803.0
52	71933.5
53	51064.0
54	36970.0
55	22876.0
56	18157.5
57	13439.0
58	11365.0
59	9291.0
60	8585.5
61	7880.0
62	6577.0
63	5274.0
64	4641.5
65	4009.0
66	3291.5
67	2574.0
68	2252.5
69	1931.0
70	1616.5
71	1302.0
72	1163.0
73	1024.0
74	801.0
75	453.5
76	329.0
77	236.0
78	143.0
79	106.5
80	70.0
81	45.0
82	20.0
83	17.0
84	14.0
85	9.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2329382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.532164125753908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17763447588088	10.84976908942325
2	7.791714807841907	2.108775272015675
3	2.6358914287177533	1.0700794629322978
4	1.3703306027303	0.7417415449075877
5	0.9311400430558938	0.6300169943346958
6	0.665754241927225	0.5405457401505649
7	0.4977548462975314	0.47149901921413234
8	0.3951384893132897	0.42776631118319147
9	0.3397679829399043	0.4138016498837225
>10	3.0765254464713507	8.68922845359746
>50	0.505703014404489	4.865822143865717
>100	1.439087883032301	51.93957362094793
>500	0.15126504634660767	13.15503749811927
>1k	0.021973238311401955	3.8211478148497346
>5k	3.1845272915075295E-4	0.275195384574781
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6386	0.2741499676738294	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2941	0.1262566637846433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017772954371588687	0.0
2	0.0	0.0	0.0	0.06709934222896889	0.0
3	0.0	0.0	0.0	0.09354412457896558	0.0
4	0.0	0.0	0.0	0.13115066571305178	0.0
5	0.0	0.0	0.0	0.2200583674124725	0.0
6	0.0	0.0	0.0	0.30218315415848496	0.0
7	0.0	0.0	0.0	0.3626713007999547	0.0
8	0.0	0.0	0.0	0.501291758930051	0.0
9	0.0	0.0	0.0	0.5575727811067485	0.0
10	0.0	0.0	0.0	0.6465234126476465	0.0
11	0.0	0.0	0.0	0.7531611388771786	0.0
12	0.0	0.0	0.0	0.8461471755169396	0.0
13	0.0	0.0	0.0	0.8850845417368212	0.0
14	0.0	0.0	0.0	0.898865020851024	0.0
15	0.0	0.0	0.0	0.9220900650902256	0.0
16	0.0	0.0	0.0	0.9795301929868093	0.0
17	0.0	0.0	0.0	1.050450291107255	0.0
18	0.0	0.0	0.0	1.1587622811544007	0.0
19	0.0	0.0	0.0	1.2050835801083721	0.0
20	0.0	0.0	0.0	1.2571574778202974	0.0
21	0.0	0.0	0.0	1.3308250857952881	0.0
22	0.0	0.0	0.0	1.4012729556594838	0.0
23	0.0	0.0	0.0	1.4951605189702677	0.0
24	0.0	0.0	0.0	1.5625174402481001	0.0
25	4.2929841477267364E-5	0.0	0.0	1.6173388478145705	0.0
26	4.2929841477267364E-5	0.0	0.0	1.6760668709554722	0.0
27	4.2929841477267364E-5	0.0	0.0	1.7333782093276242	0.0
28	4.2929841477267364E-5	0.0	0.0	1.7947249527986393	0.0
29	4.2929841477267364E-5	0.0	0.0	1.865473331553176	0.0
30	4.2929841477267364E-5	0.0	0.0	1.967474634903163	0.0
31	4.2929841477267364E-5	0.0	0.0	2.0653546734713326	0.0
32	4.2929841477267364E-5	0.0	0.0	2.1422420195571186	0.0
33	4.2929841477267364E-5	0.0	0.0	2.2212329278752905	0.0
34	4.2929841477267364E-5	0.0	0.0	2.313274508002552	0.0
35	4.2929841477267364E-5	0.0	0.0	2.4587208109275336	0.0
36	4.2929841477267364E-5	0.0	0.0	2.5555276034587715	0.0
37	4.2929841477267364E-5	0.0	0.0	2.649844465184328	0.0
38	4.2929841477267364E-5	0.0	0.0	2.7418001856286347	0.0
39	4.2929841477267364E-5	4.2929841477267364E-5	0.0	2.835473099732032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	100	0.0	45.000004	2
CTATGCG	85	0.0	45.000004	1
ATCTACG	25	3.892043E-5	45.000004	1
CAACGTA	25	3.892043E-5	45.000004	27
GCGCGTC	25	3.892043E-5	45.000004	38
CGTGACG	25	3.892043E-5	45.000004	19
TCGATAG	75	0.0	45.000004	1
ACGGGCC	25	3.892043E-5	45.000004	5
TATTGCG	270	0.0	45.000004	1
ATTCCCG	25	3.892043E-5	45.000004	45
CCCGTAA	25	3.892043E-5	45.000004	20
TCGTGAC	25	3.892043E-5	45.000004	18
TCTACGA	25	3.892043E-5	45.000004	12
GTATGCG	25	3.892043E-5	45.000004	1
AACGCTC	25	3.892043E-5	45.000004	44
CGCGTCA	25	3.892043E-5	45.000004	39
CGACTCC	25	3.892043E-5	45.000004	23
CGCTTCG	25	3.892043E-5	45.000004	32
TGATCGC	20	7.03478E-4	45.0	21
TTGATCG	20	7.03478E-4	45.0	40
>>END_MODULE