FastQCFastQC Report
Thu 26 May 2016
SRR1545087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545087_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2423760
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG85290.3518912763639965No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG81690.3370383206258045No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75530.31162326302934285No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG69260.2857543651186586No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA36910.15228405452685084No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA28450.11737960854209988No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28410.1172145757005644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC600.045.0000046
TCGTTGC502.1827873E-1145.00000412
CGTGAAT302.1665019E-645.00000425
GAGTACG253.89209E-545.0000041
TAGCGAA302.1665019E-645.0000041
CCGTGTC253.89209E-545.00000439
CCTGTCG253.89209E-545.00000418
ACGATAT502.1827873E-1145.00000425
CTAACGG253.89209E-545.0000042
CCGTACA253.89209E-545.00000433
TACGCAT253.89209E-545.00000423
GTTAACG1000.045.0000041
ACGGATC253.89209E-545.00000433
ACCACGG302.1665019E-645.0000042
ATGCGTA253.89209E-545.00000426
TTCGGAC253.89209E-545.00000433
CAGTACG600.045.0000041
AGTAGCG302.1665019E-645.0000041
TATGTCG253.89209E-545.0000041
CCGTTAG302.1665019E-645.0000041