##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545087_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2423760
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.965815097204345	31.0	31.0	33.0	30.0	33.0
2	31.29590842327623	31.0	31.0	33.0	30.0	34.0
3	31.457066293692446	31.0	31.0	34.0	30.0	34.0
4	35.293263771990624	37.0	35.0	37.0	33.0	37.0
5	35.16019407862164	37.0	35.0	37.0	32.0	37.0
6	35.23369310492788	37.0	35.0	37.0	32.0	37.0
7	35.59246872627653	36.0	35.0	37.0	35.0	37.0
8	35.65522081394197	37.0	35.0	37.0	35.0	37.0
9	37.5063409743539	39.0	37.0	39.0	35.0	39.0
10	37.107583259068555	39.0	37.0	39.0	33.0	39.0
11	36.6280419678516	39.0	35.0	39.0	32.0	39.0
12	34.94281818331848	35.0	34.0	39.0	31.0	39.0
13	34.40652333564379	35.0	33.0	39.0	27.0	39.0
14	34.98998580717563	35.0	33.0	40.0	27.0	41.0
15	35.501342542165894	35.0	33.0	40.0	30.0	41.0
16	35.74785911146318	35.0	34.0	40.0	32.0	41.0
17	35.632199557711985	35.0	34.0	40.0	31.0	41.0
18	35.77578019275836	36.0	35.0	39.0	31.0	41.0
19	35.64038766214477	36.0	35.0	39.0	31.0	41.0
20	35.326982457008945	35.0	34.0	39.0	31.0	41.0
21	35.157072482424	35.0	34.0	39.0	30.0	41.0
22	34.988863171271085	35.0	34.0	39.0	30.0	41.0
23	34.99012567250883	35.0	34.0	39.0	30.0	41.0
24	35.09520744628181	35.0	34.0	39.0	30.0	41.0
25	35.01258829257022	35.0	34.0	39.0	30.0	41.0
26	34.79771800838367	35.0	34.0	39.0	29.0	41.0
27	34.760968082648446	35.0	34.0	39.0	29.0	41.0
28	34.91523665709476	35.0	34.0	39.0	29.0	41.0
29	34.95196884179952	36.0	34.0	39.0	29.0	41.0
30	34.923138016965375	36.0	34.0	39.0	29.0	41.0
31	34.613546308215334	35.0	34.0	39.0	29.0	41.0
32	34.43815806845562	35.0	34.0	39.0	29.0	41.0
33	34.20732704558207	35.0	33.0	39.0	27.0	41.0
34	34.05997210944978	35.0	34.0	39.0	27.0	41.0
35	33.85622957718586	35.0	33.0	39.0	25.0	41.0
36	33.55874385252665	35.0	33.0	38.0	23.0	41.0
37	33.60788073076542	35.0	33.0	38.0	23.0	41.0
38	33.68061895567218	35.0	33.0	38.0	24.0	41.0
39	33.70587681948708	35.0	33.0	38.0	24.0	41.0
40	33.34535226260026	35.0	33.0	38.0	23.0	41.0
41	33.49428697560815	35.0	33.0	38.0	23.0	41.0
42	33.48684729511173	35.0	33.0	38.0	23.0	41.0
43	33.458540862131564	35.0	33.0	38.0	23.0	41.0
44	33.43770051490247	35.0	33.0	38.0	23.0	41.0
45	33.50425248374427	35.0	33.0	38.0	23.0	41.0
46	33.55976375548734	35.0	33.0	38.0	23.0	41.0
47	33.50652539855431	35.0	33.0	38.0	23.0	41.0
48	33.56921436115787	35.0	33.0	38.0	23.0	41.0
49	33.637346519457374	35.0	33.0	38.0	24.0	41.0
50	33.38858632867941	35.0	33.0	38.0	24.0	40.0
51	33.08812877512625	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	6.0
13	8.0
14	15.0
15	60.0
16	170.0
17	520.0
18	1224.0
19	2638.0
20	4629.0
21	7648.0
22	11711.0
23	17398.0
24	24823.0
25	34547.0
26	44580.0
27	51520.0
28	57758.0
29	66032.0
30	79606.0
31	99373.0
32	129513.0
33	175580.0
34	338485.0
35	509072.0
36	116488.0
37	142190.0
38	204340.0
39	303641.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.90690497408984	26.341882034524872	31.861075353995442	12.89013763738984
2	29.764786942601578	28.157697131729215	30.546299963692775	11.531215961976434
3	28.050755850414234	26.087525167508335	33.62593656137571	12.23578242070172
4	26.99891903488794	28.50529755421329	31.586625738521967	12.909157672376804
5	22.946908934878042	34.14401590916592	31.03582862989735	11.873246526058685
6	20.78386473908308	42.62080404000396	28.05950259101561	8.535828629897349
7	86.09713833052778	6.106421427864145	6.626192362280094	1.1702478793279862
8	88.96747202693336	3.9811697527808034	6.122677162755388	0.9286810575304486
9	84.55259596659735	6.354837112585404	7.2180001320262726	1.8745667887909694
10	67.15891837475658	16.93389609532297	10.602081064131761	5.305104465788692
11	65.71380829785127	14.186346833019773	14.666716176519126	5.43312869260983
12	56.713866059345804	21.70256130970063	15.882719411162821	5.700853219790739
13	16.133858137769415	60.63178697560815	16.13336303924481	7.100991847377629
14	8.409867313595406	58.80260421823943	24.956101264151567	7.831427204013599
15	8.494983001617323	20.63611908769845	62.84054526850843	8.028352642175793
16	8.749257352213089	15.073810938376736	60.39517113905667	15.781760570353502
17	8.309238538469154	16.774639403241245	29.829892398587322	45.08622965970228
18	15.392448097171338	22.000115522989073	46.597311615011385	16.010124764828202
19	37.595141433145194	17.413935373139257	27.3647143281513	17.626208865564248
20	36.2510314552596	20.280019473875303	28.031694557216884	15.437254513648217
21	16.009134567778986	38.22795986401294	29.65693798065815	16.105967587549923
22	17.810921873452816	25.425289632636893	19.77081064131762	36.99297785259267
23	12.087046572267882	36.88566524738423	24.319445819718126	26.707842360629762
24	9.893141235105785	19.8376076839291	59.33487639040169	10.934374690563423
25	8.365886061326204	25.11193352477143	51.88488134138694	14.63729907251543
26	7.524672409809552	47.39103706637621	28.46420437667096	16.620086147143283
27	9.486211506089711	55.662070502029906	25.50099844869129	9.350719543189093
28	7.606652473842295	36.24372875202165	45.57266396012807	10.576954814007987
29	7.760091758259893	27.43675116348153	43.396747202693334	21.406409875565238
30	13.157944680991518	47.868848400831766	27.756914876060335	11.21629204211638
31	37.50548734198105	30.372107799452092	23.97898306763046	8.143421790936396
32	40.44703270950919	21.25235171799188	29.099910882265572	9.200704690233357
33	39.44082747466746	27.27274977720566	18.927534079281777	14.358888668845099
34	18.572465920718223	27.118939168894613	22.588292570221473	31.72030234016569
35	19.557711984684953	23.613559098260552	37.19109977885599	19.637629138198502
36	42.38678747070667	25.63500511601809	21.479395649734297	10.498811763540946
37	15.161855959335908	49.311771792586725	25.977407003993797	9.548965244083572
38	15.563999735947453	48.22845496253754	19.721300788856983	16.48624451265802
39	20.126538931247318	42.843350826814536	24.235361586955804	12.79474865498234
40	30.88387464105357	23.324297785259265	22.634543024061788	23.157284549625377
41	12.067448922335544	24.09363963428722	26.036695052315412	37.80221639106182
42	15.090850579265275	19.937411294847674	25.680265372809192	39.29147275307786
43	17.102353368320294	19.266759085057927	28.97238175396904	34.65850579265274
44	10.48424761527544	31.902498597220845	37.28360068653662	20.329653100967093
45	10.464856256395022	58.014613658117966	19.57252368221276	11.94800640327425
46	17.918234478661255	46.852246096973296	21.24389378486319	13.985625639502262
47	17.53383173251477	30.076245172789385	23.920231376043834	28.46969171865201
48	18.15253985543123	27.591964550945637	41.31613691124534	12.939358682377794
49	22.688632537875037	27.60376439911542	39.033443905337165	10.674159157672376
50	17.91732679803281	49.06488266164967	21.518921015282043	11.498869525035483
51	16.46540911641417	43.37541670792488	22.17550417533089	17.983670000330065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2493.0
1	3675.5
2	4858.0
3	10257.5
4	15657.0
5	12943.0
6	10229.0
7	10965.5
8	11702.0
9	12438.0
10	13174.0
11	13318.5
12	13463.0
13	12775.0
14	12087.0
15	11226.5
16	10366.0
17	9985.0
18	9604.0
19	10549.5
20	11495.0
21	12188.5
22	12882.0
23	12339.0
24	11796.0
25	13819.0
26	23117.0
27	30392.0
28	41280.5
29	52169.0
30	58068.5
31	63968.0
32	76133.5
33	88299.0
34	88045.5
35	87792.0
36	84792.0
37	81792.0
38	115561.0
39	149330.0
40	226252.0
41	303174.0
42	363043.0
43	422912.0
44	397932.5
45	372953.0
46	306349.0
47	239745.0
48	191792.5
49	143840.0
50	122997.0
51	102154.0
52	74994.0
53	47834.0
54	37077.5
55	26321.0
56	20007.5
57	13694.0
58	12189.0
59	10684.0
60	9964.5
61	9245.0
62	7864.0
63	6483.0
64	5705.0
65	4927.0
66	4141.5
67	3356.0
68	2946.5
69	2537.0
70	2096.5
71	1656.0
72	1409.5
73	1163.0
74	1016.0
75	662.0
76	455.0
77	333.0
78	211.0
79	143.0
80	75.0
81	56.5
82	38.0
83	34.5
84	31.0
85	18.5
86	6.0
87	5.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2423760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.417043214823982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4675302862198	10.796363312403685
2	7.686813637414175	2.0626862151496863
3	2.668554255601654	1.074123233055295
4	1.3956070674822576	0.7489968134129287
5	0.9055507598778262	0.6074906839248744
6	0.636866236710629	0.5126917092005298
7	0.4765005366534252	0.44752598045160774
8	0.37966073034417497	0.40751395407995444
9	0.3236534588753343	0.39082251999218554
>10	2.995553374773709	8.439288040430965
>50	0.4840192431395172	4.680596361678995
>100	1.3947149547216644	50.03290429816665
>500	0.15722913945799907	13.623971289870395
>1k	0.02651314900664298	4.88542862153597
>5k	0.0012331697212392084	1.2895969666462923
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8529	0.3518912763639965	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8169	0.3370383206258045	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7553	0.31162326302934285	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6926	0.2857543651186586	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3691	0.15228405452685084	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2845	0.11737960854209988	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2841	0.1172145757005644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011634815328250322	0.0
2	0.0	0.0	0.0	0.045260256791101426	0.0
3	0.0	0.0	0.0	0.0645690992507509	0.0
4	0.0	0.0	0.0	0.10359936627388851	0.0
5	0.0	0.0	0.0	0.18479552430933754	0.0
6	0.0	0.0	0.0	0.26322738224906755	0.0
7	0.0	0.0	0.0	0.32391820972373503	0.0
8	0.0	0.0	0.0	0.4581311681024524	0.0
9	0.0	0.0	0.0	0.5105290952899627	0.0
10	0.0	0.0	0.0	0.5956447833118791	0.0
11	0.0	0.0	0.0	0.6914463478232168	0.0
12	0.0	0.0	0.0	0.7782123642604878	0.0
13	0.0	0.0	0.0	0.8122503878271776	0.0
14	0.0	0.0	0.0	0.8247928837838729	0.0
15	0.0	0.0	0.0	0.8451331815031191	0.0
16	0.0	0.0	0.0	0.8946842921741427	0.0
17	0.0	0.0	0.0	0.951084265768888	0.0
18	0.0	0.0	0.0	1.0467207974386903	0.0
19	0.0	0.0	0.0	1.0868650361421923	0.0
20	0.0	0.0	0.0	1.132042776512526	0.0
21	0.0	0.0	0.0	1.1992524012278443	0.0
22	0.0	0.0	0.0	1.260190777964815	0.0
23	0.0	0.0	0.0	1.3406030300029705	0.0
24	0.0	0.0	0.0	1.3961365811796547	0.0
25	0.0	0.0	0.0	1.4439961052249397	0.0
26	0.0	0.0	0.0	1.4975080040928144	0.0
27	0.0	0.0	0.0	1.544253556457735	0.0
28	0.0	0.0	0.0	1.5935158596560715	0.0
29	0.0	0.0	0.0	1.6518962273492426	0.0
30	0.0	0.0	0.0	1.7432831633495065	0.0
31	0.0	0.0	0.0	1.8319883156748193	0.0
32	0.0	0.0	0.0	1.9037776017427468	0.0
33	0.0	0.0	0.0	1.9755256296002905	0.0
34	0.0	0.0	0.0	2.0568455622668913	0.0
35	0.0	0.0	0.0	2.1856949532957057	0.0
36	0.0	0.0	0.0	2.273905007096412	0.0
37	0.0	0.0	0.0	2.3602171832194605	0.0
38	0.0	0.0	0.0	2.4371637455853716	0.0
39	0.0	0.0	0.0	2.51963890814272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	60	0.0	45.000004	6
TCGTTGC	50	2.1827873E-11	45.000004	12
CGTGAAT	30	2.1665019E-6	45.000004	25
GAGTACG	25	3.89209E-5	45.000004	1
TAGCGAA	30	2.1665019E-6	45.000004	1
CCGTGTC	25	3.89209E-5	45.000004	39
CCTGTCG	25	3.89209E-5	45.000004	18
ACGATAT	50	2.1827873E-11	45.000004	25
CTAACGG	25	3.89209E-5	45.000004	2
CCGTACA	25	3.89209E-5	45.000004	33
TACGCAT	25	3.89209E-5	45.000004	23
GTTAACG	100	0.0	45.000004	1
ACGGATC	25	3.89209E-5	45.000004	33
ACCACGG	30	2.1665019E-6	45.000004	2
ATGCGTA	25	3.89209E-5	45.000004	26
TTCGGAC	25	3.89209E-5	45.000004	33
CAGTACG	60	0.0	45.000004	1
AGTAGCG	30	2.1665019E-6	45.000004	1
TATGTCG	25	3.89209E-5	45.000004	1
CCGTTAG	30	2.1665019E-6	45.000004	1
>>END_MODULE