##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545086_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1469286
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.842658951354604	31.0	31.0	33.0	28.0	33.0
2	31.157149799290266	31.0	31.0	33.0	30.0	34.0
3	31.275654297393427	31.0	31.0	34.0	28.0	34.0
4	35.16004372191663	35.0	35.0	37.0	32.0	37.0
5	35.040789199652075	37.0	35.0	37.0	32.0	37.0
6	35.10711325092596	37.0	35.0	37.0	32.0	37.0
7	35.499343218406764	36.0	35.0	37.0	35.0	37.0
8	35.55678336280343	37.0	35.0	37.0	35.0	37.0
9	37.3728913227241	39.0	37.0	39.0	35.0	39.0
10	36.91344843685981	39.0	37.0	39.0	32.0	39.0
11	36.50495070394736	38.0	35.0	39.0	32.0	39.0
12	35.2623845867993	35.0	35.0	39.0	31.0	39.0
13	34.82435618388796	35.0	34.0	39.0	30.0	39.0
14	35.476994267964166	36.0	33.0	40.0	28.0	41.0
15	35.906485871368815	36.0	34.0	40.0	30.0	41.0
16	36.07549857549858	36.0	34.0	40.0	31.0	41.0
17	35.88927411000989	36.0	34.0	40.0	31.0	41.0
18	35.974957224121106	36.0	35.0	40.0	31.0	41.0
19	35.86126867063322	36.0	35.0	40.0	31.0	41.0
20	35.57612200756014	36.0	34.0	40.0	30.0	41.0
21	35.44234342394878	35.0	34.0	40.0	30.0	41.0
22	35.273033296444666	35.0	34.0	40.0	29.0	41.0
23	35.18156642069685	35.0	34.0	40.0	29.0	41.0
24	35.31038953614205	35.0	34.0	40.0	30.0	41.0
25	35.171529572867364	35.0	34.0	39.0	30.0	41.0
26	34.90522199217851	35.0	34.0	39.0	29.0	41.0
27	34.87669521114337	35.0	34.0	39.0	29.0	41.0
28	34.93800594302267	36.0	34.0	39.0	29.0	41.0
29	34.912524178410465	36.0	34.0	39.0	29.0	41.0
30	34.87301859542662	36.0	34.0	39.0	29.0	41.0
31	34.64043215548232	35.0	34.0	39.0	27.0	41.0
32	34.48375401385435	35.0	34.0	40.0	27.0	41.0
33	34.21979791545009	35.0	33.0	40.0	25.0	41.0
34	33.98637093118698	35.0	33.0	40.0	24.0	41.0
35	33.77392896958114	35.0	33.0	40.0	23.0	41.0
36	33.52615147765649	35.0	33.0	39.0	23.0	41.0
37	33.57562448699572	35.0	33.0	39.0	23.0	41.0
38	33.60262535680596	35.0	33.0	39.0	23.0	41.0
39	33.572651614457634	35.0	33.0	39.0	23.0	41.0
40	33.29967412743333	35.0	32.0	39.0	23.0	41.0
41	33.3382459235302	35.0	32.0	39.0	23.0	41.0
42	33.30422395639787	35.0	32.0	39.0	23.0	41.0
43	33.25420102008731	35.0	32.0	39.0	23.0	41.0
44	33.21548221380997	35.0	32.0	39.0	23.0	41.0
45	33.29354121661814	35.0	33.0	39.0	23.0	41.0
46	33.3154314408495	35.0	33.0	39.0	23.0	41.0
47	33.23788561246755	35.0	32.0	39.0	23.0	41.0
48	33.29032604952337	35.0	33.0	39.0	23.0	41.0
49	33.33251933251933	35.0	32.0	39.0	23.0	41.0
50	33.164066083798524	35.0	32.0	39.0	23.0	41.0
51	32.861591956909685	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	2.0
13	11.0
14	10.0
15	71.0
16	167.0
17	487.0
18	1056.0
19	2158.0
20	3870.0
21	5958.0
22	9015.0
23	12680.0
24	17717.0
25	24020.0
26	30471.0
27	34122.0
28	37295.0
29	41292.0
30	49552.0
31	59615.0
32	75439.0
33	98810.0
34	173531.0
35	250719.0
36	89388.0
37	107479.0
38	152084.0
39	192205.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.75584195316637	23.796115936584165	28.646499047836844	14.801543062412629
2	33.22035328724292	25.121589670084653	29.586547479524068	12.071509563148359
3	27.824399061857257	25.29977145361761	34.31598749324502	12.559841991280118
4	26.1125471827813	27.302376800704558	32.849901244550075	13.73517477196407
5	21.968425480131167	31.87037785699993	32.595900321652834	13.565296341216074
6	20.730545312485113	39.720449252221826	30.2717782650893	9.277227170203759
7	84.8181361559288	4.9869120103233815	8.644334731291254	1.5506171024565674
8	86.16293900574837	3.7042481858535368	8.367737799175927	1.7650750092221663
9	81.78142308577091	5.394797200817267	10.263216283283173	2.5605634301286475
10	57.25433986303552	20.122767112733666	14.963186200644394	7.659706823586422
11	51.99899815284431	17.77421141969637	21.739266555320068	8.487523872139256
12	46.67893112709166	22.01041866593706	22.221201318191287	9.089448888779993
13	18.260570100034982	48.41807517392802	22.536592603482237	10.784762122554763
14	11.072520938741674	47.6035979380461	29.4750647593457	11.848816363866531
15	10.103887194188198	23.773179626022436	55.40555072327648	10.717382456512892
16	11.452977840937708	17.43275305148215	53.537976949348185	17.576292158231958
17	11.953969479053091	18.646199582654432	32.44582742910502	36.95400350918746
18	17.148941730881532	22.876553645784416	43.53815390604689	16.43635071728717
19	33.30345487536123	19.90422558984432	29.32417514357314	17.468144391221312
20	32.32835540527848	21.727083767217547	30.19555076411264	15.749010063391333
21	17.764478801268098	33.55929342551416	31.438807692988295	17.237420080229445
22	18.939539340877136	24.61304334214033	24.375513004275547	32.07190431270699
23	13.010673211342109	34.56093640040128	28.687131028268155	23.741259359988458
24	11.860114368475573	21.093170424274103	53.442896753933546	13.603818453316782
25	11.221096505377442	26.107442662626607	47.1007006124063	15.570760219589651
26	11.24566626238867	41.07634592584425	30.385234733060816	17.292753078706255
27	10.931636182472303	46.98996655518395	29.18118051897316	12.89721674337059
28	10.474951779299605	31.972740501168595	44.06283051768002	13.489477201851784
29	10.98404258939376	25.94777327218799	42.02381292682296	21.04437121159529
30	15.563681951641817	40.19857264004421	30.548919679354462	13.688825728959507
31	31.923669047414865	29.186285039127846	27.043271357652625	11.846774555804657
32	35.677465108903235	22.117681649454223	29.502220806568637	12.702632435073907
33	34.66717847988751	25.879168521308987	22.818157935214792	16.63549506358871
34	19.260307387397688	25.380695113136586	27.203893591853458	28.155103907612265
35	19.544459009341953	24.66783185846731	36.079292935480225	19.70841619671051
36	35.05362468573171	27.164078334646895	24.741541129500995	13.040755850120398
37	16.655640903132543	43.23045343112233	27.685624173918487	12.428281491826642
38	16.442748382547716	42.45340934304145	24.733237776716038	16.3706044976948
39	19.42637444309685	39.94089646263559	26.72924127773626	13.903487816531296
40	28.06301836402171	25.049718026306657	25.734744631065702	21.152518978605936
41	14.502554301885407	24.32086060848603	28.31143834488316	32.8651467447454
42	16.573764399851356	21.718440113088946	26.73250817063526	34.97528731642444
43	16.450711433989028	23.109864246987993	29.245225231847304	31.19419908717568
44	13.015165189078232	30.864855446795247	35.7074796874128	20.412499676713725
45	12.545549334846996	48.923082367898424	23.14491528538351	15.38645301187107
46	17.17507687407353	42.39678319945879	24.40117172558644	16.026968200881246
47	16.120755251190033	29.424495979679925	27.924856018501504	26.529892750628537
48	17.954707252366113	25.82791913895593	40.8638617668718	15.353511841806156
49	20.26930087130756	27.9864505617014	38.15376992634518	13.590478640645864
50	17.043992796501158	43.20819772324789	25.79749619883399	13.95031328141696
51	15.313968825674513	39.96322023077876	26.360218500686727	18.362592442860002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1577.0
1	2511.5
2	3446.0
3	9436.5
4	15427.0
5	11975.0
6	8523.0
7	8684.0
8	8845.0
9	9648.0
10	10451.0
11	10427.0
12	10403.0
13	10148.0
14	9893.0
15	9436.5
16	8980.0
17	8250.0
18	7520.0
19	7710.5
20	7901.0
21	8898.5
22	9896.0
23	9763.5
24	9631.0
25	11074.5
26	16930.5
27	21343.0
28	23839.5
29	26336.0
30	31765.0
31	37194.0
32	41430.5
33	45667.0
34	53188.5
35	60710.0
36	64899.0
37	69088.0
38	80147.5
39	91207.0
40	121880.5
41	152554.0
42	185310.5
43	218067.0
44	204693.5
45	191320.0
46	160153.5
47	128987.0
48	108880.0
49	88773.0
50	76218.5
51	63664.0
52	50628.5
53	37593.0
54	30552.0
55	23511.0
56	20619.5
57	17728.0
58	16722.5
59	15717.0
60	14830.5
61	13944.0
62	12527.0
63	11110.0
64	10014.0
65	8918.0
66	7406.5
67	5895.0
68	5481.5
69	5068.0
70	4648.5
71	4229.0
72	3323.0
73	2417.0
74	1980.0
75	1163.5
76	784.0
77	589.5
78	395.0
79	323.0
80	251.0
81	186.5
82	122.0
83	89.5
84	57.0
85	54.0
86	51.0
87	32.0
88	13.0
89	9.0
90	5.0
91	4.5
92	4.0
93	6.5
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1469286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.3972468862093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91230666289779	15.500787415866968
2	6.445261517338609	2.5004065779600193
3	2.426348843066974	1.4119346262311518
4	1.5335833126802647	1.1898917654651922
5	1.0751673503622883	1.04276432694844
6	0.8229741242433548	0.9578059361346144
7	0.6567324462230312	0.8917160978300614
8	0.4954940579793692	0.7688976458620423
9	0.43239423528124465	0.7548531960531576
>10	3.9087933923491485	16.033351903911125
>50	0.9883747093077315	13.987655514193072
>100	1.288832137728702	42.650443120491396
>500	0.011976029702863008	1.4046755641113569
>1k	0.0014089446709250597	0.3326018116685678
>5k	3.5223616773126493E-4	0.5722144972728543
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8375	0.5700047506067573	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1473	0.10025277583806012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009324256815895613	0.0
2	0.0	0.0	0.0	0.03532327947043666	0.0
3	0.0	0.0	0.0	0.054244034177144546	0.0
4	0.0	0.0	0.0	0.09188136278437281	0.0
5	0.0	0.0	0.0	0.17307726337826673	0.0
6	0.0	0.0	0.0	0.2681574587929103	0.0
7	0.0	0.0	0.0	0.34166254902040855	0.0
8	0.0	0.0	0.0	0.5119493413807795	0.0
9	0.0	0.0	0.0	0.5900825298818609	0.0
10	0.0	0.0	0.0	0.7060572277963583	0.0
11	0.0	0.0	0.0	0.7998442781051477	0.0
12	0.0	0.0	0.0	0.887846205571958	0.0
13	0.0	0.0	0.0	0.9203109537557698	0.0
14	0.0	0.0	0.0	0.9331062842768528	0.0
15	0.0	0.0	0.0	0.9527076416708524	0.0
16	0.0	0.0	0.0	0.9955856109702264	0.0
17	0.0	0.0	0.0	1.0473114152043919	0.0
18	0.0	0.0	0.0	1.1234027956435984	0.0
19	0.0	0.0	0.0	1.1589302559202226	0.0
20	0.0	0.0	0.0	1.2018762854883256	0.0
21	0.0	0.0	0.0	1.2644917327191574	0.0
22	0.0	0.0	0.0	1.3307824344613641	0.0
23	0.0	0.0	0.0	1.404968127376154	0.0
24	0.0	0.0	0.0	1.4580551369849029	0.0
25	0.0	0.0	0.0	1.5091683988005058	0.0
26	0.0	0.0	0.0	1.5596691181975464	0.0
27	0.0	0.0	0.0	1.6145256947932534	0.0
28	0.0	0.0	0.0	1.6714240794508353	0.0
29	0.0	0.0	0.0	1.7400288303298337	0.0
30	0.0	0.0	0.0	1.837491135149998	0.0
31	0.0	0.0	0.0	1.9231109532112876	0.0
32	0.0	0.0	0.0	1.9981133693508275	0.0
33	0.0	0.0	0.0	2.082031680693888	0.0
34	0.0	0.0	0.0	2.1662902933805945	0.0
35	0.0	0.0	0.0	2.282945593982383	0.0
36	0.0	0.0	0.0	2.3748950170354854	0.0
37	0.0	0.0	0.0	2.473718527230233	0.0
38	0.0	0.0	0.0	2.587515296545397	0.0
39	0.0	0.0	0.0	2.7639275130913927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.1659162E-6	45.000004	1
TCGTTAG	30	2.1659162E-6	45.000004	1
CGTTAGG	130	0.0	45.000004	2
GGTACCG	20	7.0339296E-4	45.0	24
CCGATTA	35	1.2122291E-7	45.0	12
AGCCCGT	25	3.8913386E-5	45.0	16
CGTTGAA	35	1.2122291E-7	45.0	17
CACGACA	20	7.0339296E-4	45.0	17
TCGATTG	80	0.0	45.0	1
CGACAAG	40	6.8175723E-9	45.0	18
CGTTCGA	45	3.8562575E-10	45.0	14
GCGTTAG	95	0.0	45.0	1
TCTAGCG	90	0.0	45.0	1
ACTACCG	20	7.0339296E-4	45.0	28
CCGAATT	25	3.8913386E-5	45.0	29
ACGTACG	25	3.8913386E-5	45.0	1
TAAATCG	20	7.0339296E-4	45.0	34
CCCGGTA	20	7.0339296E-4	45.0	28
CGATCGT	35	1.2122291E-7	45.0	10
TGTCGAT	20	7.0339296E-4	45.0	21
>>END_MODULE