FastQCFastQC Report
Thu 26 May 2016
SRR1545085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545085_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3499710
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155660.44477971031885494No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG116370.33251326538484616No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG104440.2984247266202057No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG69310.1980449808698435No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG55770.15935606093076285No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA50780.14509773666960976No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG40980.11709541647736527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40360.11532384111826409No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA39700.11343797057470477No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36880.10538016007040583No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA36370.10392289646856454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA302.1667802E-645.0000046
CGACGAA302.1667802E-645.00000432
CGGCATA207.0352654E-445.00000426
CAACCGT351.212884E-745.00000420
TTCGTCA207.0352654E-445.00000413
CGCGATT207.0352654E-445.00000428
TACGACA207.0352654E-445.00000430
TCGTCAA207.0352654E-445.00000414
ACTAACG351.212884E-745.00000426
TAGTCCG351.212884E-745.0000041
GCGTAAA406.8212103E-945.0000041
AACCCGT253.8924467E-545.044
CGCATTA253.8924467E-545.014
CGTTCGA253.8924467E-545.014
CAAGTCG650.044.9999961
GCAACCG1150.044.9999961
TGTAGCG1700.043.6764681
AGTACGG4500.043.52
CGAGGGT4100.043.353664
TAGTACG2650.043.3018881