FastQCFastQC Report
Thu 26 May 2016
SRR1545084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545084_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1435900
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98710.6874434152796155No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37330.25997632147085453No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24020.16728184413956404No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18480.12869977017898182No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17170.11957657218469252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG253.891292E-545.0000041
GTTAGCG1000.045.0000041
CGAATTA253.891292E-545.00000441
GTATCGT253.891292E-545.00000416
TCGAGAG502.1827873E-1145.0000041
ATACGTT253.891292E-545.00000436
TCGACCA253.891292E-545.00000437
CGTTTAC253.891292E-545.00000433
TCGGTAT253.891292E-545.00000435
GCGTAAG1000.045.0000041
TGCGTGA253.891292E-545.00000440
TAATCCG253.891292E-545.0000041
CTATGCG700.045.01
TAGCGAT207.0338754E-445.037
CCTACGC207.0338754E-445.013
CTAAGCG351.2122109E-745.01
AAACGCC351.2122109E-745.011
AATGCGT207.0338754E-445.038
ACGGCAC207.0338754E-445.035
GTTCGAA406.8175723E-945.015