##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545084_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1435900
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.845404276063793	31.0	31.0	33.0	28.0	33.0
2	31.154913991225015	31.0	31.0	33.0	30.0	34.0
3	31.266703809457482	31.0	31.0	34.0	28.0	34.0
4	35.139279894143044	35.0	35.0	37.0	32.0	37.0
5	35.015859043108854	35.0	35.0	37.0	32.0	37.0
6	35.10212340692249	37.0	35.0	37.0	32.0	37.0
7	35.51584441813497	36.0	35.0	37.0	35.0	37.0
8	35.567135594400725	37.0	35.0	37.0	35.0	37.0
9	37.34007451772408	39.0	37.0	39.0	35.0	39.0
10	36.89413399261787	39.0	37.0	39.0	32.0	39.0
11	36.530624695313044	38.0	35.0	39.0	32.0	39.0
12	35.38306079810572	37.0	35.0	39.0	31.0	39.0
13	34.97732432620656	37.0	34.0	39.0	30.0	39.0
14	35.67673793439655	37.0	34.0	40.0	30.0	41.0
15	36.03705271954872	37.0	34.0	40.0	31.0	41.0
16	36.17014416045686	37.0	34.0	40.0	31.0	41.0
17	35.98716066578452	36.0	34.0	40.0	31.0	41.0
18	36.0508113378369	36.0	35.0	40.0	31.0	41.0
19	35.91846159203287	36.0	35.0	40.0	31.0	41.0
20	35.66806532488335	36.0	34.0	40.0	30.0	41.0
21	35.538102235531724	35.0	34.0	40.0	30.0	41.0
22	35.36141862246674	35.0	34.0	40.0	30.0	41.0
23	35.26613343547601	35.0	34.0	40.0	29.0	41.0
24	35.33537850825267	35.0	34.0	40.0	30.0	41.0
25	35.22175987185737	35.0	34.0	40.0	29.0	41.0
26	34.95882930566196	35.0	34.0	39.0	29.0	41.0
27	34.938323699421964	35.0	34.0	39.0	29.0	41.0
28	34.99209763911136	36.0	34.0	39.0	29.0	41.0
29	34.97318128003343	36.0	34.0	39.0	29.0	41.0
30	34.93483808064629	36.0	34.0	39.0	29.0	41.0
31	34.71694198760359	35.0	34.0	40.0	27.0	41.0
32	34.546295703043384	35.0	34.0	40.0	27.0	41.0
33	34.29987394665367	35.0	33.0	40.0	25.0	41.0
34	34.05108155164009	35.0	33.0	40.0	24.0	41.0
35	33.82928059057038	35.0	33.0	40.0	23.0	41.0
36	33.59225712096943	35.0	33.0	40.0	22.0	41.0
37	33.672257817396755	35.0	33.0	40.0	23.0	41.0
38	33.66801100355178	35.0	33.0	40.0	23.0	41.0
39	33.63023191030016	35.0	33.0	40.0	23.0	41.0
40	33.38966641130998	35.0	32.0	39.0	23.0	41.0
41	33.3834535831186	35.0	32.0	39.0	23.0	41.0
42	33.33922069782018	35.0	32.0	39.0	23.0	41.0
43	33.285391043944564	35.0	32.0	39.0	22.0	41.0
44	33.2668869698447	35.0	32.0	39.0	22.0	41.0
45	33.31094087331987	35.0	32.0	39.0	23.0	41.0
46	33.31192422870674	35.0	32.0	39.0	23.0	41.0
47	33.22594191796086	35.0	32.0	39.0	23.0	41.0
48	33.22916498363396	35.0	32.0	39.0	22.0	41.0
49	33.281952085799844	35.0	32.0	39.0	22.0	41.0
50	33.11014903544815	35.0	32.0	39.0	22.0	41.0
51	32.802282192353225	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	7.0
13	5.0
14	17.0
15	51.0
16	162.0
17	439.0
18	1029.0
19	2028.0
20	3690.0
21	5768.0
22	8716.0
23	12531.0
24	17130.0
25	23329.0
26	30167.0
27	34172.0
28	37191.0
29	41152.0
30	48309.0
31	58211.0
32	72735.0
33	94781.0
34	162756.0
35	234151.0
36	89631.0
37	107692.0
38	152311.0
39	197667.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.08280520927641	23.849362768995057	28.095828400306427	14.972003621422106
2	33.33470297374469	26.245142419388536	28.577338254753116	11.842816352113656
3	29.124939062608817	25.068110592659654	33.36966362560067	12.43728671913086
4	27.135037258862038	26.826032453513477	32.595654293474475	13.443275994150012
5	23.1169997910718	32.16561041855282	31.440768855769903	13.276620934605473
6	20.33400654641688	40.82582352531514	30.042621352461868	8.797548575806115
7	83.10376767184344	5.426004596420364	9.5592311442301	1.9109965875060937
8	84.0520231213873	4.76718434431367	9.231840657427398	1.9489518768716483
9	78.59837036005293	7.495438401002856	10.976321470854517	2.9298697680897
10	53.86531095480187	22.54676509506233	15.492652691691621	8.095271258444182
11	51.15453722404067	18.81544675813079	21.242426352810085	8.787589665018455
12	45.60805069990946	22.80980569677554	22.157253290619124	9.42489031269587
13	18.16428720662999	48.08127306915523	22.727975485758062	11.026464238456716
14	11.774357545790098	47.69426840309214	28.875827007451772	11.655547043665994
15	11.607145344383314	24.399958214360332	53.7078487359844	10.285047705271955
16	11.936764398635002	19.43408315342294	51.8215753186155	16.807577129326553
17	12.233442440281356	19.61278640573856	32.01761961139355	36.13615154258653
18	17.206629988160735	23.496900898391253	42.86572881119855	16.43074030224946
19	32.8967894700188	20.428511734800473	28.595166794345012	18.079532000835712
20	31.751236158506863	22.971933978689325	29.944703670172018	15.332126192631797
21	17.508879448429557	35.01420711748729	30.54279545929382	16.93411797478933
22	19.011212479977715	26.17821575318616	24.191447872414514	30.61912389442162
23	15.151264015599972	34.289713768368266	28.07542307960164	22.48359913643011
24	12.660282749495092	22.359356501149104	51.0970123267637	13.883348422592103
25	11.986558952573299	27.19116930148339	45.30858694895188	15.513684796991434
26	11.57768646841702	41.622188174663975	29.80625391740372	16.99387143951529
27	12.603175708614806	45.151821157462216	28.812103906957308	13.432899226965667
28	11.028901734104046	32.97088933769761	42.923392993941086	13.07681593425726
29	11.786196810362839	26.63604707848736	41.25809596768577	20.31966014346403
30	15.34716902291246	41.0766766487917	29.912389442161714	13.663764886134134
31	32.0247231701372	28.62434709938018	26.832857441326	12.518072289156626
32	33.926526916916224	24.401977853610976	29.40232606727488	12.269169162197926
33	32.672400584998954	28.137056898112682	23.49759732571906	15.692945191169303
34	18.70694338045825	26.497666968451846	27.44418134967616	27.35120830141375
35	20.261856675255938	24.367574343617243	35.62197924646563	19.748589734661188
36	33.89038233860296	28.105508740162964	24.533811546765094	13.470297374468974
37	16.300229821018178	42.61940246535274	28.531931192980014	12.54843652064907
38	15.94735009401769	42.87526986558953	23.950205446061705	17.227174594331082
39	20.32383870743088	38.204331777978965	27.159830071732017	14.311999442858136
40	27.08447663486315	25.576432899226965	26.14360331499408	21.195487150915802
41	14.293613761403998	25.276690577338258	28.26457274183439	32.165122919423354
42	16.080576641827427	23.579566822202104	26.596420363535067	33.7434361724354
43	17.389302876244862	23.59857928825127	29.48269378090396	29.5294240545999
44	14.214499616964972	31.017689254126328	35.074865937739396	19.6929451911693
45	13.046730273695939	47.86189846089561	23.700745177240755	15.3906260881677
46	17.2554495438401	42.1115676579149	24.308865519883	16.324117278362003
47	18.050351695800543	29.477052719548713	26.819277108433738	25.653318476217006
48	18.017271397729647	26.8546556166864	39.547391879657354	15.580681105926597
49	19.923532279406643	28.24695313044084	37.2583745386169	14.571140051535622
50	17.567240058499895	42.671773800403926	25.549063305244097	14.211922835852079
51	16.755066508809804	38.45657775611115	25.969635768507555	18.818719966571486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1603.0
1	2593.0
2	3583.0
3	10953.0
4	18323.0
5	13832.5
6	9342.0
7	9525.0
8	9708.0
9	10171.0
10	10634.0
11	10439.0
12	10244.0
13	9864.0
14	9484.0
15	9107.5
16	8731.0
17	8187.5
18	7644.0
19	7578.0
20	7512.0
21	7948.5
22	8385.0
23	8952.5
24	9520.0
25	11749.0
26	16611.0
27	19244.0
28	25699.0
29	32154.0
30	36198.0
31	40242.0
32	45228.0
33	50214.0
34	53520.0
35	56826.0
36	60831.5
37	64837.0
38	72143.0
39	79449.0
40	113795.0
41	148141.0
42	173881.0
43	199621.0
44	190454.5
45	181288.0
46	155772.0
47	130256.0
48	108733.5
49	87211.0
50	74514.5
51	61818.0
52	50147.5
53	38477.0
54	31419.0
55	24361.0
56	22450.0
57	20539.0
58	19817.0
59	19095.0
60	17211.0
61	15327.0
62	14108.5
63	12890.0
64	10595.5
65	8301.0
66	6860.5
67	5420.0
68	4547.5
69	3675.0
70	3239.5
71	2804.0
72	2432.0
73	2060.0
74	1654.5
75	1009.0
76	769.0
77	611.5
78	454.0
79	337.5
80	221.0
81	187.5
82	154.0
83	104.0
84	54.0
85	42.0
86	30.0
87	24.5
88	19.0
89	11.0
90	3.0
91	1.5
92	0.0
93	1.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1435900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.537618698323293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54345110328126	16.33633068707914
2	6.604909922539578	2.7129824305177985
3	2.6213997386484067	1.6151192486473591
4	1.545454053329765	1.2695978425225962
5	1.0852057280532603	1.1143770725997089
6	0.8057069032285656	0.9928380696669089
7	0.6470774117309886	0.9302600405300296
8	0.5454261101966423	0.8961402783462642
9	0.4368439560535936	0.8074561140086223
>10	4.062147795275751	17.42859656530887
>50	0.9516346219582108	14.191502812203339
>100	1.1347459373313118	38.334314448157755
>500	0.012593161272122911	1.584095509052808
>1k	0.0030632013905163838	1.0963965254387649
>5k	3.40355710057376E-4	0.6899923559200541
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9871	0.6874434152796155	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3733	0.25997632147085453	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2402	0.16728184413956404	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1848	0.12869977017898182	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1717	0.11957657218469252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03029458875966293	0.0
2	0.0	0.0	0.0	0.10404624277456648	0.0
3	0.0	0.0	0.0	0.14659795250365623	0.0
4	0.0	0.0	0.0	0.21415140330106552	0.0
5	0.0	0.0	0.0	0.38442788495020547	0.0
6	0.0	0.0	0.0	0.5678668430949231	0.0
7	0.0	0.0	0.0	0.7005362490424124	0.0
8	0.0	0.0	0.0	0.9926178703252315	0.0
9	0.0	0.0	0.0	1.1117069433804583	0.0
10	0.0	0.0	0.0	1.299254822759245	0.0
11	0.0	0.0	0.0	1.4582491816978898	0.0
12	0.0	0.0	0.0	1.5983703600529284	0.0
13	0.0	0.0	0.0	1.6575666829166376	0.0
14	0.0	0.0	0.0	1.6804791420015321	0.0
15	0.0	0.0	0.0	1.7198272860227035	0.0
16	0.0	0.0	0.0	1.801657497040184	0.0
17	0.0	0.0	0.0	1.8947698307681593	0.0
18	0.0	0.0	0.0	2.0275785221812104	0.0
19	0.0	0.0	0.0	2.0854516331220836	0.0
20	0.0	0.0	0.0	2.153422940316178	0.0
21	0.0	0.0	0.0	2.2492513406226062	0.0
22	0.0	0.0	0.0	2.3554565081133783	0.0
23	6.964273278083432E-5	0.0	0.0	2.4764955776864683	0.0
24	6.964273278083432E-5	0.0	0.0	2.567518629431019	0.0
25	6.964273278083432E-5	0.0	0.0	2.6431506372310047	0.0
26	6.964273278083432E-5	0.0	0.0	2.7248415627829234	0.0
27	6.964273278083432E-5	0.0	0.0	2.8022842816352114	0.0
28	6.964273278083432E-5	0.0	0.0	2.8862037746361167	0.0
29	6.964273278083432E-5	0.0	0.0	2.9747196880005573	0.0
30	6.964273278083432E-5	0.0	0.0	3.102444459920607	0.0
31	6.964273278083432E-5	0.0	0.0	3.223274601295355	0.0
32	6.964273278083432E-5	0.0	0.0	3.336235113865868	0.0
33	6.964273278083432E-5	0.0	0.0	3.4429277804861065	0.0
34	6.964273278083432E-5	0.0	0.0	3.5574900759105788	0.0
35	6.964273278083432E-5	0.0	0.0	3.715509436590292	0.0
36	6.964273278083432E-5	0.0	0.0	3.843652064907027	0.0
37	6.964273278083432E-5	0.0	0.0	3.9719339786893237	0.0
38	6.964273278083432E-5	0.0	0.0	4.090326624416742	0.0
39	6.964273278083432E-5	0.0	0.0	4.215892471620586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	3.891292E-5	45.000004	1
GTTAGCG	100	0.0	45.000004	1
CGAATTA	25	3.891292E-5	45.000004	41
GTATCGT	25	3.891292E-5	45.000004	16
TCGAGAG	50	2.1827873E-11	45.000004	1
ATACGTT	25	3.891292E-5	45.000004	36
TCGACCA	25	3.891292E-5	45.000004	37
CGTTTAC	25	3.891292E-5	45.000004	33
TCGGTAT	25	3.891292E-5	45.000004	35
GCGTAAG	100	0.0	45.000004	1
TGCGTGA	25	3.891292E-5	45.000004	40
TAATCCG	25	3.891292E-5	45.000004	1
CTATGCG	70	0.0	45.0	1
TAGCGAT	20	7.0338754E-4	45.0	37
CCTACGC	20	7.0338754E-4	45.0	13
CTAAGCG	35	1.2122109E-7	45.0	1
AAACGCC	35	1.2122109E-7	45.0	11
AATGCGT	20	7.0338754E-4	45.0	38
ACGGCAC	20	7.0338754E-4	45.0	35
GTTCGAA	40	6.8175723E-9	45.0	15
>>END_MODULE